Rabbit Polyclonal IP10 antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Rat, Recombinant full length protein - Human samples. Cited in 38 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL10.
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Publications
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- American journal of translational research 15:2676-26892023CXCL5 knockdown attenuated gemcitabine resistance of pancreatic cancer through regulation of cancer cells and tumour stroma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNien-Hung Lee,Yi Ma,Ching-Seng Ang,Chelsea Dumesny,Nhi Huynh,Yang Yang,Kai Wang,Mehrdad Nikfarjam,Hong HePubMed 37193135
- Thoracic cancer 14:881-8922023Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR-6838-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Zhang,Jiami Huang,Jiayun Wang,Yongheng Li,Guohua Hu,He LiPubMed 36811283
- Frontiers in pharmacology 13:9059662022Curcumenol Mitigates the Inflammation and Ameliorates the Catabolism Status of the Intervertebral Discs Inhibiting the TNFα/NFκB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Yang,Baixing Li,Haijun Tian,Xiaofei Cheng,Tangjun Zhou,Jie ZhaoPubMed 35795557
- Nature protocols 17:190-2212022Routine culture and study of adult human brain cells from neurosurgical specimens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas I-H Park,Leon C D Smyth,Miranda Aalderink,Zoe R Woolf,Justin Rustenhoven,Kevin Lee,Deidre Jansson,Amy Smith,Sheryl Feng,Jason Correia,Peter Heppner,Patrick Schweder,Edward Mee,Mike DragunowPubMed 35022619
- Journal of the European Academy of Dermatology and Venereology : JEADV 36:144-1532021Epigallocatechin-3-gallate exhibits anti-inflammatory effects in a human interface dermatitis model-implications for therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Braegelmann,D Niebel,S Ferring-Schmitt,T Fetter,J Landsberg,M Hölzel,M Effern,N Glodde,S Steinbuch,T Bieber,J WenzelPubMed 34585800
- Scientific reports 11:180072021Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilim Kim,Hye Yeon Choi,Ji Won Woo,Yul Ri Chung,So Yeon ParkPubMed 34504204
- Diagnostics (Basel, Switzerland) 11:2021Active Ratio Test (ART) as a Novel Diagnostic for Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Woog Kang,Adam Rainczuk,Martin K Oehler,Thomas W Jobling,Magdalena Plebanski,Andrew N StephensPubMed 34200333
- Archives of dermatological research 314:275-2842021Systemic CXCL10 is a predictive biomarker of vitiligo lesional skin infiltration, PUVA, NB-UVB and corticosteroid treatment response and outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM El-Domyati,W H El-Din,A F Rezk,I Chervoneva,J B Lee,M Farber,J Uitto,O Igoucheva,Vitali AlexeevPubMed 33866437
- Frontiers in molecular neuroscience 13:5220732020The Interplay Between Beta-Amyloid 1-42 (Aβ)-Induced Hippocampal Inflammatory Response, p-tau, Vascular Pathology, and Their Synergistic Contributions to Neuronal Death and Behavioral Deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeatriz Calvo-Flores Guzmán et. al.PubMed 33224025
- National science review 7:1868-18782020Pathological changes in the lungs and lymphatic organs of 12 COVID-19 autopsy cases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Liu,Yu Shi,Jun Cai,Yaqi Duan,Rongshuai Wang,Hongyan Zhang,Qiurong Ruan,Jiansha Li,Lei Zhao,Yifang Ping,Rong Chen,Liang Ren,Xiaochun Fei,Heng Zhang,Rui Tang,Xi Wang,Tao Luo,Xindong Liu,Xuequan Huang,Zhenhua Liu,Qilin Ao,Yong Ren,Jing Xiong,Zhicheng He,Haibo Wu,Wenjuan Fu,Pengnan Zhao,Xinwei Chen,Guoqiang Qu,Yunyun Wang,Xi Wang,Jia Liu,Dongfang Xiang,Sanpeng Xu,Xiaowei Zhou,Qingrui Li,Jinghong Ma,Heng Li,Jie Zhang,Sizhe Huang,Xiaohong Yao,Yiwu Zhou,Chaofu Wang,Dingyu Zhang,Guoping Wang,Liang Liu,Xiu-Wu BianPubMed 34676085
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CXCL10. Database link P02778
Consider this alternative
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Tested |
| Recombinant full length protein - Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info 0.1-0.2 µg/mL | Notes To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes To detect hIP-10 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-IP10 antibody (ab271283), allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Rat | Dilution info - | Notes To detect hIP-10 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-IP10 antibody (ab271283), allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed:11157474, PubMed:22652417, PubMed:7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed:12750173, PubMed:19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed:12663757, PubMed:12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
Alternative names
INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10
Recommended products
Rabbit Polyclonal IP10 antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Rat, Recombinant full length protein - Human samples. Cited in 38 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL10.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CXCL10. Database link P02778
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Although some customers have been successful in IHC we no longer batch test in this application.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
- Biological function summary
IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.
- Pathways
The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.
- Associated diseases and disorders
IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Sandwich ELISA - Anti-IP10 antibody (ab9807)
ab9807 detecting IP-10 by sandwich ELISA.
Western blot - Anti-IP10 antibody (ab9807)
All lanes: Western blot - Anti-IP10 antibody (ab9807) at 1 µg/mL
All lanes: Western blot - Recombinant rat IP10 protein (Recombinant rat IP10 protein ab75477) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 10 kDa
Exposure time: 30s
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