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Rabbit Polyclonal IP10 antibody. Suitable for WB and reacts with Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Cxcl10.


Images

Western blot - Anti-IP10 antibody (AB9938), expandable thumbnail

Publications

Key facts

Isotype

Unknown

Host species

Rabbit

Storage buffer

Standard buffer

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Mouse Cxcl10. Database link P17515

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Target data

Function

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed:28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed:18624292, PubMed:19017990, PubMed:28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed:15456824).

Alternative names

Recommended products

Rabbit Polyclonal IP10 antibody. Suitable for WB and reacts with Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Cxcl10.

Key facts

Isotype

Unknown

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Mouse Cxcl10. Database link P17515
Purification technique

Affinity purification Immunogen

Specificity

This antibody does not cross-react with murine CXCL9 and CXCL11.

Light chain type

unknown

Reconstitution

Reconstitute with 200µl of sterile water.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.

Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

Associated diseases and disorders

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.

Product promise

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1 product image

  • Western blot - Anti-IP10 antibody (ab9938), expandable thumbnail

    Western blot - Anti-IP10 antibody (ab9938)

    Western blot performed with ab9938 at 0.2 ug/ml

    Lane 1: MultiMark MultiColor Standard (Invitrogen)

    Lane 2: 250 ng Murine IP-10

    Lane 3: 125 ng Murine IP-10

    Lane 4: 62.5 ng Murine IP-10

    Lane 5: 31.25 ng Murine IP-10

    Lane 6: 15.625 ng Murine IP-10

    Lane 7: 7.8 ng Murine IP-10

    Lane 8: 3.9 ng Murine IP-10

    Lane 9: 1.95 ng Murine IP-10

    Lane 10: 0.975 ng Murine IP-10

    Lane 11: 0.4875 ng Murine IP-10

    Lane 12 0.24 ng Murine IP-10

    All lanes: Western blot - Anti-IP10 antibody (ab9938)

    Predicted band size: 10 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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