JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325792

Anti-IP10 antibody [EPR30290-22]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal IP10 antibody. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell line - Mouse, Mouse samples.

View Alternative Names

Crg2, Ifi10, Inp10, Scyb10, Cxcl10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, C7, Interferon-gamma induced protein CRG-2, Small-inducible cytokine B10, Gamma-IP10, IP-10

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR30290-22] (AB325792)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IP10 antibody [EPR30290-22] (AB325792)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) and RAW 264.7 treated with mIFN-gamma (200 ng/mL) and lipopolysaccharide (50 ng/mL) for 24 hr cells labelling IP10 with ab325792 at 1/200 (2.51 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic staining in RAW 264.7 cells (shown in green) treated with mIFN-gamma (200 ng/mL) and lipopolysaccharide (50 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

Flow Cytometry (Intracellular) - Anti-IP10 antibody [EPR30290-22] (AB325792)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IP10 antibody [EPR30290-22] (AB325792)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T cells transfected with a mouse Cxcl10 expression vector containing a myc-His-tag® (Middle) and 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling IP10 with ab325792 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

Flow Cytometry (Intracellular) - Anti-IP10 antibody [EPR30290-22] (AB325792)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IP10 antibody [EPR30290-22] (AB325792)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse bone marrow treated with 100ng/mL M-CSF for 6days (Upper left and right)and Mouse bone marrow treated with 100ng/mL M-CSF for 6days then serum starvation for 2 hours then 50ng/mL IFN beta (Mouse) for 4hours (Lower left and right) cells labelling IP10 with ab325792 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD11b conjugated to BV421. Then fixed with 4% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.

Flow Cytometry (Intracellular) - Anti-IP10 antibody [EPR30290-22] (AB325792)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IP10 antibody [EPR30290-22] (AB325792)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 200ng/ml mIFN-r and 50ng/ml LPS for 3+21hours then add 1µg/ml BFA for 21h (Lower left and right) and Untreated RAW 264.7 (Upper left and right) cells labelling IP10 with ab325792 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD86 conjugated to APC/CY7. Then fixed with 4% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30290-22

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell line - Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (By similarity) (PubMed : 28623423). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (PubMed : 18624292, PubMed : 19017990, PubMed : 28468883). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (By similarity). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (PubMed : 15456824).
See full target information Cxcl10
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com