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Rabbit Recombinant Monoclonal IP3 receptor antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Expected
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Not recommended
Expected
Tested
Rat
Not recommended
Expected
Not recommended
Tested
Expected

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate (PubMed:27108797). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal IP3 receptor antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4537

Purification technique

Affinity purification Protein A

Dissociation constant

2.1 x 10-12 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

IP3 receptor involves in various cellular processes by mediating intracellular calcium release. Upon binding of IP3 produced by phospholipase C catalysis of PIP2 the channel opens to allow calcium ions to flow into the cytosol. The IP3 receptor forms a tetrameric complex essential for its function. This complex is vital for responses in signal transduction pathways affecting T-cell activation muscle contraction and neurotransmitter release.

Activity summary

The IP3 receptor (IP3R) also known as inositol 145-trisphosphate receptor functions as a ligand-gated calcium channel. It plays a major role in calcium release from the endoplasmic reticulum into the cytoplasm. The IP3 receptor has a molecular mass of approximately 313 kDa. IP3 receptor expression is extensive found in various tissues including the brain liver and smooth muscle cells. It is an essential component of intracellular calcium signaling mechanisms directly activated by IP3 a secondary messenger molecule.

Pathways

IP3 receptor is central to calcium signaling and interacts with the IP3 pathway modulating cellular activities in response to different stimuli. The interaction with proteins such as TRPC3 a transient receptor potential channel integrates calcium influx. IP3 receptor also participates in the calcium/calmodulin-dependent protein kinase (CaMK) pathway impacting phosphorylation events in the cell.

Associated diseases and disorders

Dysregulation of IP3 receptor function associates with neurodegenerative diseases such as Alzheimer's Disease due to calcium homeostasis disruption. The receptor also connects to heart diseases where abnormal calcium signaling can lead to arrhythmic conditions. The interaction between the IP3 receptor and other proteins like ryanodine receptors may exacerbate these conditions influencing cellular calcium dynamics in pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517), expandable thumbnail

    Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: IP3 receptor knockout HAP1 cell lysate (20 μg)
    Lane 3: Human brain tissue lysate (20 μg)
    Lane 4: SH-SY5Y cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.


    ab108517 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-IP3 receptor antibody [EPR4537] (AB108517)

    Predicted band size: 314 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517), expandable thumbnail

    Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    ab108517 (purified) at 1:500 dilution (1.124 μg/ml) immunoprecipitating IP3 receptor in Mouse brain lysate.
    Lane 1 (input): Mouse brain lysate 10μg
    Lane 2 (+): ab108517 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (AB108517)

    Predicted band size: 314 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517), expandable thumbnail

    Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    All lanes: Western blot - Anti-IP3 receptor antibody [EPR4537] (AB108517) at 1/1000 dilution

    Lane 1: Rat brain lysate at 10 µg

    Lane 2: SH-SY5Y lysate at 10 µg

    Lane 3: Mouse brain lysate at 10 µg

    Lane 4: HeLa brain lysate at 10 µg

    Predicted band size: 314 kDa

  • OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517), expandable thumbnail

    OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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