Anti-IP3 receptor antibody [L24/18]

• Flow Cyt

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Flow Cytometry - Anti-IP3 receptor antibody [L24/18] (AB252536)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SH-SY5Y cells labeling IP3 receptor with ab252536 at 10.825μg/ml (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (AB252536)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling IP3 receptor with ab252536 at 0.108μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on human cerebellum is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252536 for 30 mins at RT.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (AB252536)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling IP3 receptor with ab252536 at 0.108μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on mouse cerebellum is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252536 for 30 mins at RT.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [L24/18] (AB252536)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling IP3 receptor with ab252536 at 0.108μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on rat cerebellum is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab252536 for 30 mins at RT.

The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (AB252536)

Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling IP3 receptor with ab252536 at 4.33μg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-IP3 receptor antibody [L24/18] (AB252536)

Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling IP3 receptor with ab252536 at 4.33μg/ml, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody isb150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20.

• WB

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Western blot - Anti-IP3 receptor antibody [L24/18] (AB252536)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3.25 seconds.

All lanes:

Western blot - Anti-IP3 receptor antibody [L24/18] (ab252536) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

Predicted band size: 314 kDa

Observed band size: 314 kDa

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