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AB308165

Anti-IP3 Receptor I antibody [HL1541]

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(1 Publication)

Rabbit Recombinant Monoclonal IP3 receptor antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITPR1.

View Alternative Names

INSP3R1, ITPR1, IP3 receptor isoform 1, IP3R 1, InsP3R1, Type 1 InsP3 receptor

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 Receptor I antibody [HL1541] (AB308165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 Receptor I antibody [HL1541] (AB308165)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum labeling IP3 Receptor I protein with ab308165 at 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 Receptor I antibody [HL1541] (AB308165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 Receptor I antibody [HL1541] (AB308165)

For immunofluorescence analysis, DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of IP3 Receptor I protein using ab308165 at 1/250 (green).

Nuclei (blue) is stained with fluoroshield with DAPI.

Tau, an axon marker, stained by Tau antibody (red) diluted at 1/500

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 Receptor I antibody [HL1541] (AB308165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 Receptor I antibody [HL1541] (AB308165)

Immunohistochemical analysis of paraffin-embedded rat cerebellum labeling IP3 Receptor I protein with ab308165 at 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Western blot - Anti-IP3 Receptor I antibody [HL1541] (AB308165)
  • WB

Supplier Data

Western blot - Anti-IP3 Receptor I antibody [HL1541] (AB308165)

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (ab308165) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Rat cerebellum tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1541

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ITPR1. The exact immunogen used to generate this antibody is proprietary information.

Q14643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IP3 receptor (IP3R) also known as inositol 145-trisphosphate receptor functions as a ligand-gated calcium channel. It plays a major role in calcium release from the endoplasmic reticulum into the cytoplasm. The IP3 receptor has a molecular mass of approximately 313 kDa. IP3 receptor expression is extensive found in various tissues including the brain liver and smooth muscle cells. It is an essential component of intracellular calcium signaling mechanisms directly activated by IP3 a secondary messenger molecule.
Biological function summary

IP3 receptor involves in various cellular processes by mediating intracellular calcium release. Upon binding of IP3 produced by phospholipase C catalysis of PIP2 the channel opens to allow calcium ions to flow into the cytosol. The IP3 receptor forms a tetrameric complex essential for its function. This complex is vital for responses in signal transduction pathways affecting T-cell activation muscle contraction and neurotransmitter release.

Pathways

IP3 receptor is central to calcium signaling and interacts with the IP3 pathway modulating cellular activities in response to different stimuli. The interaction with proteins such as TRPC3 a transient receptor potential channel integrates calcium influx. IP3 receptor also participates in the calcium/calmodulin-dependent protein kinase (CaMK) pathway impacting phosphorylation events in the cell.

Dysregulation of IP3 receptor function associates with neurodegenerative diseases such as Alzheimer's Disease due to calcium homeostasis disruption. The receptor also connects to heart diseases where abnormal calcium signaling can lead to arrhythmic conditions. The interaction between the IP3 receptor and other proteins like ryanodine receptors may exacerbate these conditions influencing cellular calcium dynamics in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER) (PubMed : 10620513, PubMed : 27108797). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane (By similarity). Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex (By similarity). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations (By similarity).
See full target information ITPR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diabetes & metabolism journal 48:1058-1072 PubMed39069376

2024

PDZD8 Augments Endoplasmic Reticulum-Mitochondria Contact and Regulates Ca2+ Dynamics and Cypd Expression to Induce Pancreatic β-Cell Death during Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxin Liu,Yongqing Wei,Xiaolong Jin,Hongyu Cai,Qianqian Chen,Xiujuan Zhang
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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