JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264280

Anti-IP3R1 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal IP3 receptor antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ITPR1 aa 1000-1050.

View Alternative Names

INSP3R1, ITPR1, IP3 receptor isoform 1, IP3R 1, InsP3R1, Type 1 InsP3 receptor

2 Images
Immunoprecipitation - Anti-IP3R1 antibody (AB264280)
  • IP

Supplier Data

Immunoprecipitation - Anti-IP3R1 antibody (AB264280)

IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264280 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264280 at 0.4 μg/ml.

Lane 1 : ab264280 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 15 secs.

All lanes:

Immunoprecipitation - Anti-IP3R1 antibody (ab264280)

false

Western blot - Anti-IP3R1 antibody (AB264280)
  • WB

Supplier Data

Western blot - Anti-IP3R1 antibody (AB264280)

3-8% SDS-PAGE

All lanes:

Western blot - Anti-IP3R1 antibody (ab264280) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 314 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ITPR1 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information.

Q14643

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP3R1 or inositol 145-trisphosphate receptor type 1 is a calcium channel that mediates the release of calcium ions from the endoplasmic reticulum into the cytoplasm. It plays critical roles in cellular signaling. Weighing approximately 313 kDa IP3R1 is primarily found in the brain particularly in Purkinje cells of the cerebellar cortex but it also appears in other tissues like heart and liver. This protein belongs to a family of similar receptors IP3R2 and IP3R3 which exhibit different distribution and activity in various tissue types.
Biological function summary

IP3R1 acts as a part of a tetrameric channel complex that senses the presence of inositol trisphosphate (IP3) to initiate calcium release. Its expression and function rely heavily on intracellular calcium regulation which is essential for multiple cellular processes like gene expression metabolism and apoptosis. IP3R1 modulates neuronal function and plasticity by influencing calcium-dependent signal transduction pathways.

Pathways

IP3R1 plays critical roles in the phosphoinositide signaling pathway and the calcium signaling pathway. It interacts with proteins such as phospholipase C which synthesizes IP3 and calmodulin which plays a role in feedback mechanisms for calcium channel activity. These interactions facilitate IP3R1’s function in calcium wave propagation and neurotransmitter release in neuronal cells.

IP3R1 mutations and dysregulation link to neurological diseases like spinocerebellar ataxia and Alzheimer’s disease. Cross-talk with other proteins like presenilin 1 involved in Alzheimer's suggests that alterations in IP3R1 can impact calcium homeostasis in neurodegenerative disorders. Spinocerebellar ataxia specifically connects to defects in calcium signaling that IP3R1 mediates which is pivotal for cerebellar neuron function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER) (PubMed : 10620513, PubMed : 27108797). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane (By similarity). Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex (By similarity). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations (By similarity).
See full target information ITPR1
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB264281

Anti-IP3R1 antibody

Immunoprecipitation - Anti-IP3R1 antibody (AB264281)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Recombinant|Lab Essentials

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB264283

Anti-ITPR3 antibody

Immunoprecipitation - Anti-ITPR3 antibody (AB264283)

  • 0
  • 0
Primary Antibodies

AB178693

Anti-IRBIT antibody [EPR13409]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (AB178693)

  • 0
  • 0
Primary Antibodies

AB129201

Anti-Grp75/MOT antibody [EPR5326(2)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Grp75/MOT antibody [EPR5326(2)] (AB129201)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com