Rabbit Polyclonal IP3 receptor antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ITPR1 aa 2700 to C-terminus.
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- Endocrine connections 13:2023Mfn2 regulates mitochondria-associated ER membranes to affect PCOS oocyte development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuhua Liao,Suqin Zhu,Shumin Qiu,Hua Cao,Wenwen Jiang,Huiling Xu,Yan Sun,Beihong ZhengPubMed 37988456
- Life science alliance 7:2023ESYT1 tethers the ER to mitochondria and is required for mitochondrial lipid and calcium homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre Janer,Jordan L Morris,Michiel Krols,Hana Antonicka,Mari J Aaltonen,Zhen-Yuan Lin,Hanish Anand,Anne-Claude Gingras,Julien Prudent,Eric A ShoubridgePubMed 37931956
- IBRO neuroscience reports 14:273-2832023Neuroprotective properties of anti-apoptotic BCL-2 proteins in 5xFAD mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Chernyuk,M Callens,M Polozova,A Gordeev,M Chigriai,A Rakovskaya,A Ilina,E Pchitskaya,C Van den Haute,T Vervliet,G Bultynck,I BezprozvannyPubMed 36926591
- PloS one 18:e02790292023Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxin Luo,Ling Li,Muyao Ding,Yurong Niu,Xuezhu Xu,Xiaoxia Shi,Ning Shan,Zewen Qiu,Fengyuan Piao,Cong ZhangPubMed 36656826
- Nature communications 13:74552022TRPC channels blockade abolishes endotoxemic cardiac dysfunction by hampering intracellular inflammation and Ca leakage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Tang,Wen Tian,Guang-Yuan Ma,Xiong Xiao,Lei Zhou,Ze-Zhi Li,Xiao-Xiao Liu,Chong-Yao Li,Ke-Han Wu,Wenjuan Liu,Xue-Ying Wang,Yuan-Yuan Gao,Xin Yang,Jianzhao Qi,Ding Li,Yang Liu,Wen-Sheng Chen,Jinming Gao,Xiao-Qiang Li,Wei CaoPubMed 36460692
- Frontiers in aging neuroscience 14:9906792022Sevoflurane exposure causes neuronal apoptosis and cognitive dysfunction by inducing ER stress activation of the inositol 1, 4, 5-trisphosphate receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Zhang,Yanan Li,Xupeng Wang,Chunping Yin,Qi Zhou,Junfei Guo,Juan Zhao,Xiaohui Xian,Zhiyong Hou,Qiujun WangPubMed 36337694
- Atherosclerosis 334:39-472021The regulation of Ero1-alpha in homocysteine-induced macrophage apoptosis and vulnerable plaque formation in atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Zhang,Lili Zhu,Xianxian Wu,Ru Yan,Shaobing Yang,Xiaoliang Jiang,Xing Liu,Xue Liu,Ning Yan,Guangzhi Cong,Zhiwei Yang,Shaobin JiaPubMed 34478920
- Cell death discovery 7:312021IP3R1 regulates Ca transport and pyroptosis through the NLRP3/Caspase-1 pathway in myocardial ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuixi Mo,Xin Liu,Yiyue Zhong,Jian Mo,Zhiyi Li,Daheng Li,Liangqing Zhang,Yijun LiuPubMed 33568649
- Journal of biochemical and molecular toxicology 34:e224402020Vitamin D and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti-inflammatory actions by remodeling cellular calcium pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed El-Boshy,Bassem Refaat,Riyad A Almaimani,Abdelghany H Abdelghany,Jawwad Ahmad,Shakir Idris,Hussain Almasmoum,Amani A Mahbub,Mazen M Ghaith,Mohammad A BaSalamahPubMed 31926057
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ITPR1 aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14643
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER) (PubMed:10620513, PubMed:27108797). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane (By similarity). Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex (By similarity). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations (By similarity).
Alternative names
INSP3R1, ITPR1, IP3 receptor isoform 1, IP3R 1, InsP3R1, Type 1 InsP3 receptor
Recommended products
Rabbit Polyclonal IP3 receptor antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human ITPR1 aa 2700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ITPR1 aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14643
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IP3R1 or inositol 145-trisphosphate receptor type 1 is a calcium channel that mediates the release of calcium ions from the endoplasmic reticulum into the cytoplasm. It plays critical roles in cellular signaling. Weighing approximately 313 kDa IP3R1 is primarily found in the brain particularly in Purkinje cells of the cerebellar cortex but it also appears in other tissues like heart and liver. This protein belongs to a family of similar receptors IP3R2 and IP3R3 which exhibit different distribution and activity in various tissue types.
- Biological function summary
IP3R1 acts as a part of a tetrameric channel complex that senses the presence of inositol trisphosphate (IP3) to initiate calcium release. Its expression and function rely heavily on intracellular calcium regulation which is essential for multiple cellular processes like gene expression metabolism and apoptosis. IP3R1 modulates neuronal function and plasticity by influencing calcium-dependent signal transduction pathways.
- Pathways
IP3R1 plays critical roles in the phosphoinositide signaling pathway and the calcium signaling pathway. It interacts with proteins such as phospholipase C which synthesizes IP3 and calmodulin which plays a role in feedback mechanisms for calcium channel activity. These interactions facilitate IP3R1’s function in calcium wave propagation and neurotransmitter release in neuronal cells.
- Associated diseases and disorders
IP3R1 mutations and dysregulation link to neurological diseases like spinocerebellar ataxia and Alzheimer’s disease. Cross-talk with other proteins like presenilin 1 involved in Alzheimer's suggests that alterations in IP3R1 can impact calcium homeostasis in neurodegenerative disorders. Spinocerebellar ataxia specifically connects to defects in calcium signaling that IP3R1 mediates which is pivotal for cerebellar neuron function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (ab264281)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/100 dilution. Detection: Red fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstain: DAPI (blue).
Immunoprecipitation - Anti-IP3R1 antibody (ab264281)
IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264281 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264281 at 0.4 μg/ml.
Lane 1: ab264281 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
All lanes: Immunoprecipitation - Anti-IP3R1 antibody (ab264281)
Western blot - Anti-IP3R1 antibody (ab264281)
All lanes: Western blot - Anti-IP3R1 antibody (ab264281) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 314 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (ab264281)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/200 dilution. Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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