AB264281

Anti-IP3R1 antibody

Name: Anti-IP3R1 antibody (ab264281) | Abcam
Brand: Abcam Limited
SKU: AB264281
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal IP3 receptor antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human ITPR1 aa 2700 to C-terminus.

View Alternative Names

INSP3R1, ITPR1, IP3 receptor isoform 1, IP3R 1, InsP3R1, Type 1 InsP3 receptor

4 Images

Supplier Data

Immunoprecipitation - Anti-IP3R1 antibody (AB264281)

IP3R1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264281 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264281 at 0.4 μg/ml.

Lane 1 : ab264281 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 30 secs.

All lanes:

Immunoprecipitation - Anti-IP3R1 antibody (ab264281)

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (AB264281)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (AB264281)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/200 dilution. Detection : DAB. Counterstain : IHC Hematoxylin (blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (AB264281)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse cerebellum tissue labeling IP3R1 with ab264281 at 1/100 dilution. Detection : Red fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight^® 594 Conjugated used at a dilution of 1/100. Counterstain : DAPI (blue).

Supplier Data

Western blot - Anti-IP3R1 antibody (AB264281)

All lanes:

Western blot - Anti-IP3R1 antibody (ab264281) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 314 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ITPR1 aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP3R1 or inositol 145-trisphosphate receptor type 1 is a calcium channel that mediates the release of calcium ions from the endoplasmic reticulum into the cytoplasm. It plays critical roles in cellular signaling. Weighing approximately 313 kDa IP3R1 is primarily found in the brain particularly in Purkinje cells of the cerebellar cortex but it also appears in other tissues like heart and liver. This protein belongs to a family of similar receptors IP3R2 and IP3R3 which exhibit different distribution and activity in various tissue types.

Biological function summary

IP3R1 acts as a part of a tetrameric channel complex that senses the presence of inositol trisphosphate (IP3) to initiate calcium release. Its expression and function rely heavily on intracellular calcium regulation which is essential for multiple cellular processes like gene expression metabolism and apoptosis. IP3R1 modulates neuronal function and plasticity by influencing calcium-dependent signal transduction pathways.

Pathways

IP3R1 plays critical roles in the phosphoinositide signaling pathway and the calcium signaling pathway. It interacts with proteins such as phospholipase C which synthesizes IP3 and calmodulin which plays a role in feedback mechanisms for calcium channel activity. These interactions facilitate IP3R1’s function in calcium wave propagation and neurotransmitter release in neuronal cells.

IP3R1 mutations and dysregulation link to neurological diseases like spinocerebellar ataxia and Alzheimer’s disease. Cross-talk with other proteins like presenilin 1 involved in Alzheimer's suggests that alterations in IP3R1 can impact calcium homeostasis in neurodegenerative disorders. Spinocerebellar ataxia specifically connects to defects in calcium signaling that IP3R1 mediates which is pivotal for cerebellar neuron function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inositol 1,4,5-trisphosphate-gated calcium channel that, upon inositol 1,4,5-trisphosphate binding, mediates calcium release from the endoplasmic reticulum (ER) (PubMed : 10620513, PubMed : 27108797). Undergoes conformational changes upon ligand binding, suggesting structural flexibility that allows the channel to switch from a closed state, capable of interacting with its ligands such as 1,4,5-trisphosphate and calcium, to an open state, capable of transferring calcium ions across the ER membrane (By similarity). Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CAMK2 complex (By similarity). Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Regulates fertilization and egg activation by tuning the frequency and amplitude of calcium oscillations (By similarity).

See full target information ITPR1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Toxicology research 14:tfaf100 PubMed40718852

2025

Modulating intracellular calcium dynamics with alkaloids: A novel strategy against oxidative neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Serap Niğdelioğlu Dolanbay

Cell communication and signaling : CCS 22:464 PubMed39350150

2024

ER-mitochondria contact sites regulate hepatic lipogenesis via Ip3r-Grp75-Vdac complex recruiting Seipin.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jia Chi,Zhen-Yu Bai,Guang-Li Feng,Xiao-Hong Lai,Yu-Feng Song

Cell reports 43:113992 PubMed38536815

2024

OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Styliani Panagiotou,Kia Wee Tan,Phuoc My Nguyen,Andreas Müller,Affiong Ika Oqua,Alejandra Tomas,Anna Wendt,Lena Eliasson,Anders Tengholm,Michele Solimena,Olof Idevall-Hagren

Experimental biology and medicine (Maywood, N.J.) 248:2262-2272 PubMed38159072

2024

IP3R-1 aggravates endotoxin-induced acute lung injury in mice by regulating MAM formation and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Shuan Dong,Ya Wu,Yuan Zhang,Shaona Li,Qin Zhao,Shasha Liu,Yan Guo,Xiangyun Li,Kai Song,Lili Wu,Lina Wu,Jia Shi,Lirong Gong,Jianbo Yu

Endocrine connections 13: PubMed37988456

2023

Mfn2 regulates mitochondria-associated ER membranes to affect PCOS oocyte development.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuhua Liao,Suqin Zhu,Shumin Qiu,Hua Cao,Wenwen Jiang,Huiling Xu,Yan Sun,Beihong Zheng

Life science alliance 7: PubMed37931956

2023

ESYT1 tethers the ER to mitochondria and is required for mitochondrial lipid and calcium homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Janer,Jordan L Morris,Michiel Krols,Hana Antonicka,Mari J Aaltonen,Zhen-Yuan Lin,Hanish Anand,Anne-Claude Gingras,Julien Prudent,Eric A Shoubridge

Cell reports 42:112883 PubMed37498742

2023

COPI-regulated mitochondria-ER contact site formation maintains axonal integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Maddison,Bilal Malik,Leonardo Amadio,Dana M Bis-Brewer,Stephan Züchner,Owen M Peters,Gaynor A Smith

IBRO neuroscience reports 14:273-283 PubMed36926591

2023

Neuroprotective properties of anti-apoptotic BCL-2 proteins in 5xFAD mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

D Chernyuk,M Callens,M Polozova,A Gordeev,M Chigriai,A Rakovskaya,A Ilina,E Pchitskaya,C Van den Haute,T Vervliet,G Bultynck,I Bezprozvanny

PloS one 18:e0279029 PubMed36656826

2023

Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxin Luo,Ling Li,Muyao Ding,Yurong Niu,Xuezhu Xu,Xiaoxia Shi,Ning Shan,Zewen Qiu,Fengyuan Piao,Cong Zhang

Nature communications 13:7455 PubMed36460692

2022

TRPC channels blockade abolishes endotoxemic cardiac dysfunction by hampering intracellular inflammation and Ca leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Na Tang,Wen Tian,Guang-Yuan Ma,Xiong Xiao,Lei Zhou,Ze-Zhi Li,Xiao-Xiao Liu,Chong-Yao Li,Ke-Han Wu,Wenjuan Liu,Xue-Ying Wang,Yuan-Yuan Gao,Xin Yang,Jianzhao Qi,Ding Li,Yang Liu,Wen-Sheng Chen,Jinming Gao,Xiao-Qiang Li,Wei Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IP3R1 antibody

Immunoprecipitation - Anti-IP3R1 antibody (AB264281)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (AB264281)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3R1 antibody (AB264281)

Western blot - Anti-IP3R1 antibody (AB264281)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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