AB214816

Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IP6K1 phospho S118 + S121 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell line - Human samples. Cited in 1 publication.

View Alternative Names

IHPK1, KIAA0263, IP6K1, Inositol hexakisphosphate kinase 1, InsP6 kinase 1, Inositol hexaphosphate kinase 1

4 Images

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Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

IP6K1 (phospho S118 + S121) was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate with ab214816 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214816 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution

Lane 1 : MOLT-4 whole cell lysate 10 μg (Input).
Lane 2 : ab214816 IP in MOLT-4 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab214816 in MOLT-4 whole cell lysate (-).

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 minute.

All lanes:

Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

Predicted band size: 50 kDa

Observed band size: 52 kDa

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Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Blocking/diluting buffer : 5% NFDM/TBST.

The bands migrating at approximately 100 kDa are likely to be non-specific.

All lanes:

Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/5000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Lane 2:

MOLT-4 whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 52 kDa

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Exposure time: 26s

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Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Blocking/diluting buffer : 5% NFDM/TBST.

The band at approximately 30 kDa likely represents degradation fragments of IP6K1 (pS118 and pS121) protein.

The plasmids were kindly provided by our collaborator Dr. Feng Rao NIBS.

All lanes:

Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/10000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C at 10 µg

Lane 3:

HEK-293T transfected with a His-tagged human IP6K1 S118A+S121A mutant expression vector, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

false

Exposure time: 26s

Unknown

Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Dot blot analysis of IP6K1 (phospho S118 + S121) labeled with ab214816 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody was used at 1/100,000 dilution..

Lane 1 : IP6K1 (phospho S118 + S121) peptide (aa114-125).

Lane 2 : IP6K1 (phospho S118 + S121) peptide (aa116-127).

Lane 3 : IP6K1 (phospho S118) peptide (aa114-127).

Lane 4 : IP6K1 (phospho S121) peptide (aa114-127).

Lane 5 : IP6K1 non-phospho peptide (aa114-127).

Exposure time : 58 seconds.

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Carrier free

Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19852

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein inositol hexakisphosphate kinase 1 (IP6K1) also known as IHPK1 plays an important mechanical role in cellular signaling. It catalyzes the conversion of inositol hexakisphosphate (IP6) to diphosphoinositol pentakisphosphate (IP7). IP6K1 has a molecular mass of approximately 48 kDa. It is expressed in various tissues including the brain testis and pancreas where it is involved in multiple cellular processes.

Biological function summary

IP6K1 influences several critical cell functions such as energy metabolism cell migration and apoptosis. It also participates in the regulation of insulin secretion. IP6K1 does not primarily form part of a complex; however it interacts with proteins that impact these biological functions. Its role in metabolism and signaling pathways marks it as a significant contributor to maintaining cellular homeostasis.

Pathways

IP6K1 plays a part in the inositol phosphate metabolism and insulin signaling pathways. Within these pathways IP6K1 interacts closely with proteins such as Akt mTOR and other kinases modulating their activities. These interactions affect cellular energy status and influence growth factor signaling pathways impacting numerous downstream biological processes.

IP6K1's activity links it to conditions such as type 2 diabetes and obesity. In type 2 diabetes IP6K1 interacts with insulin-signaling proteins affecting glucose homeostasis and insulin sensitivity. The protein's role in obesity relates to its influence on energy metabolism as it can impact energy expenditure and fat storage with potential interactions involving with proteins like leptin and AMPK.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4.

See full target information IP6K1 phospho S118 + S121

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 3:1400-1414 PubMed34663975

2021

5-IP is a GPCR messenger mediating neural control of synaptotagmin-dependent insulin exocytosis and glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhe Zhang,Na Li,Jun Zhang,Yanshen Zhang,Xiaoli Yang,Yifan Luo,Bobo Zhang,Zhixue Xu,Zhenhua Zhu,Xiuyan Yang,Yuan Yan,Biao Lin,Shen Wang,Da Chen,Caichao Ye,Yan Ding,Mingliang Lou,Qingcui Wu,Zhanfeng Hou,Keren Zhang,Ziming Liang,Anqi Wei,Bianbian Wang,Changhe Wang,Nan Jiang,Wenqing Zhang,Guozhi Xiao,Cong Ma,Yan Ren,Xiangbing Qi,Weiping Han,Chao Wang,Feng Rao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852]

Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise