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Rabbit Recombinant Monoclonal IP6K1 phospho S118 + S121 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell line - Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816), expandable thumbnail
  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816), expandable thumbnail
  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816), expandable thumbnail
  • Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (AB214816), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWB
Human
Tested
Expected
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Transfected cell line - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/5000
Notes

-

Species
Human
Dilution info
1/5000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4.

Alternative names

IHPK1, KIAA0263, IP6K1, Inositol hexakisphosphate kinase 1, InsP6 kinase 1, Inositol hexaphosphate kinase 1

Recommended products

Rabbit Recombinant Monoclonal IP6K1 phospho S118 + S121 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell line - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19852
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein inositol hexakisphosphate kinase 1 (IP6K1) also known as IHPK1 plays an important mechanical role in cellular signaling. It catalyzes the conversion of inositol hexakisphosphate (IP6) to diphosphoinositol pentakisphosphate (IP7). IP6K1 has a molecular mass of approximately 48 kDa. It is expressed in various tissues including the brain testis and pancreas where it is involved in multiple cellular processes.

Biological function summary

IP6K1 influences several critical cell functions such as energy metabolism cell migration and apoptosis. It also participates in the regulation of insulin secretion. IP6K1 does not primarily form part of a complex; however it interacts with proteins that impact these biological functions. Its role in metabolism and signaling pathways marks it as a significant contributor to maintaining cellular homeostasis.

Pathways

IP6K1 plays a part in the inositol phosphate metabolism and insulin signaling pathways. Within these pathways IP6K1 interacts closely with proteins such as Akt mTOR and other kinases modulating their activities. These interactions affect cellular energy status and influence growth factor signaling pathways impacting numerous downstream biological processes.

Associated diseases and disorders

IP6K1's activity links it to conditions such as type 2 diabetes and obesity. In type 2 diabetes IP6K1 interacts with insulin-signaling proteins affecting glucose homeostasis and insulin sensitivity. The protein's role in obesity relates to its influence on energy metabolism as it can impact energy expenditure and fat storage with potential interactions involving with proteins like leptin and AMPK.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816), expandable thumbnail

    Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    IP6K1 (phospho S118 + S121) was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate with ab214816 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214816 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5,000 dilution


    Lane 1: MOLT-4 whole cell lysate 10 μg (Input).
    Lane 2: ab214816 IP in MOLT-4 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214816 in MOLT-4 whole cell lysate (-).

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 minute.

    All lanes: Immunoprecipitation - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    Predicted band size: 50 kDa

    Observed band size: 52 kDa

  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816), expandable thumbnail

    Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    Blocking/diluting buffer: 5% NFDM/TBST.

    The bands migrating at approximately 100 kDa are likely to be non-specific.

    All lanes: Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/5000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

    Lane 2: MOLT-4 whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Observed band size: 52 kDa

    Exposure time: 26s

  • Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816), expandable thumbnail

    Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    Blocking/diluting buffer: 5% NFDM/TBST.

    The band at approximately 30 kDa likely represents degradation fragments of IP6K1 (pS118 and pS121) protein.

    The plasmids were kindly provided by our collaborator Dr. Feng Rao NIBS.

    All lanes: Western blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816) at 1/10000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged human IP6K1 (WT) expression vector, whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with a His-tagged human IP6K1 (WT) expression vector whole cell lysate. The WB membrane was treated with alkaline phosphatase for 1h at 37°C at 10 µg

    Lane 3: HEK-293T transfected with a His-tagged human IP6K1 S118A+S121A mutant expression vector, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Exposure time: 26s

  • Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816), expandable thumbnail

    Dot Blot - Anti-IP6K1 (phospho S118 + S121) antibody [EPR19852] (ab214816)

    Dot blot analysis of IP6K1 (phospho S118 + S121) labeled with ab214816 at 1/1000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody was used at 1/100,000 dilution..

    Lane 1: IP6K1 (phospho S118 + S121) peptide (aa114-125).

    Lane 2: IP6K1 (phospho S118 + S121) peptide (aa116-127).

    Lane 3: IP6K1 (phospho S118) peptide (aa114-127).

    Lane 4: IP6K1 (phospho S121) peptide (aa114-127).

    Lane 5: IP6K1 non-phospho peptide (aa114-127).

    Exposure time: 58 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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