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AB284392

Anti-IP6K2 antibody [EPR23538-12] - BSA and Azide free

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Rabbit Recombinant Monoclonal IP6K2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

IHPK2, TCCCIA00113, IP6K2, Inositol hexakisphosphate kinase 2, InsP6 kinase 2, InsP6K2, P(i)-uptake stimulator, PiUS

1 Images
Western blot - Anti-IP6K2 antibody [EPR23538-12] - BSA and Azide free (AB284392)
  • WB

Lab

Western blot - Anti-IP6K2 antibody [EPR23538-12] - BSA and Azide free (AB284392)

This data was developed using ab252414, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

Lysates were kindly provided by the collaborator.

Lanes 1-3 : Merged signal (red and green). Green - ab252414 observed at 55kDa. Red - loading control ab8245 was observed at 36 kDa.

ab252414 Anti-IP6K2 antibody [EPR23538-12] was shown to react with IP6K2 in wild-type HCT116 cells in Western blot. Wild-type and IP6K2 knockout samples were subjected to SDS-PAGE.

ab252414 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-IP6K2 antibody [EPR23538-12] (<a href='/en-us/products/primary-antibodies/ip6k2-antibody-epr23538-12-ab252414'>ab252414</a>) at 1/1000 dilution

Lane 1:

Wild type HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate

Lane 2:

IP6K2 knockout HCT116 whole cell lysate

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 49 kDa

Observed band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23538-12

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284392 is the carrier-free version of ab252414.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IP6K2 protein also known as inositol hexakisphosphate kinase 2 functions mechanically as an enzyme that converts inositol hexakisphosphate (IP6) into diphosphoinositol pentakisphosphate (IP7). It has a molecular mass of about 55 kDa. This kinase is widely expressed in various tissues with significant levels found in the brain and testes. IP6K2 activity plays a role in cellular energy homeostasis and signaling.
Biological function summary

IP6K2 influences several cellular processes including apoptosis and cell cycle regulation. It operates as a part of protein complexes that mediate the synthesis of inositol pyrophosphates which are important secondary messengers in cells. Through these roles IP6K2 helps regulate physiological functions such as insulin signaling and energy balance. Its interactions with various binding partners also enable it to influence cell survival and growth parameters.

Pathways

IP6K2 is actively involved in the inositol phosphate metabolism and apoptosis pathways. Inositol phosphate metabolism involves a range of signaling molecules where IP6K2 contributes to the generation of key signaling lipids. It works in conjunction with other kinases such as IP6K1 and phosphatidylinositol 3-kinase (PI3K) facilitating complex cellular activities. Through these pathways IP6K2 modulates processes that are essential for maintaining cell viability and proper cellular responses to external stimuli.

Dysregulation of IP6K2 has associations with several conditions including cancer and metabolic disorders. Altered IP6K2 function can influence cancer development by affecting apoptotic pathways where it interacts with key proteins such as p53 to regulate cell death. In metabolic disorders the kinase's role in insulin signaling connects it to metabolic issues like type 2 diabetes as its involvement in signal transduction impacts glucose homeostasis and insulin sensitivity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5).
See full target information IP6K2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com