Rabbit Recombinant Monoclonal IPCEF1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-IPCEF1 antibody [EPR19419-146] - BSA and Azide free (Capture) (AB242602), expandable thumbnail](https://content.abcam.com/products/images/ipcef1-antibody-epr19419-146-bsa-and-azide-free-capture-ab242602--sandwich-elisa-img174141.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IPCEF1 antibody [EPR19419-146] - BSA and Azide free (Capture) (AB242602)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner.
Alternative names
Interactor protein for cytohesin exchange factors 1, Phosphoinositide-binding protein PIP3-E, KIAA0403, IPCEF1
Recommended products
Rabbit Recombinant Monoclonal IPCEF1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Interactor protein for cytohesin exchange factors 1, Phosphoinositide-binding protein PIP3-E, KIAA0403, IPCEF1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19419-146
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab242602 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PIP3E. The recommended pair for sandwich ELISA is:
Capture: ab242602, Human PIP3E Capture Antibody (unconjugated)
Detector: Anti-IPCEF1 antibody [EPR19419-37] - BSA and Azide free (Detector) ab242848, Human PIP3E Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- Activity summary
IPCEF1 also known as Interactor of little protein 1 or PIP3-ET is a protein involved in cellular signaling and trafficking. It has a molecular mass of about 52 kDa. IPCEF1 is expressed in various tissues but shows higher levels notably in the brain and immune cells. Its primary mechanical function is to act as an effector and regulator of small GTPases particularly influencing proteins like Arf6.
- Biological function summary
IPCEF1 contributes to signal transduction and membrane dynamics. It plays a role in forming protein complexes with EFA6 a known guanine nucleotide exchange factor to enhance the activation of Arf GTPases. These complexes facilitate cellular functions like endocytosis and cytoskeletal remodeling important for maintaining cell morphology and movement.
- Pathways
Several critical pathways involve IPCEF1 such as the Rac1 and Arf6 signaling pathways. In these pathways IPCEF1 interacts with proteins like Rac1 a small GTPase significant in actin cytoskeleton reorganization. IPCEF1's interaction with these pathways highlights its role a role in managing cellular motility and growth responses.
- Associated diseases and disorders
Recent studies indicate IPCEF1's involvement in cancer and neurodegenerative diseases. It connects to cancer through its regulation of Arf6 a protein often implicated in tumor progression and metastasis. In neurodegenerative contexts IPCEF1 could influence pathological processes through its interaction with Rac1 which affects neuronal actin dynamics and synapse functionality.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-IPCEF1 antibody [EPR19419-146] - BSA and Azide free (Capture) (ab242602), expandable thumbnail](https://content.abcam.com/products/images/ipcef1-antibody-epr19419-146-bsa-and-azide-free-capture-ab242602--sandwich-elisa-img174141.jpg "Sandwich ELISA - Anti-IPCEF1 antibody [EPR19419-146] - BSA and Azide free (Capture) (ab242602)")
Sandwich ELISA - Anti-IPCEF1 antibody [EPR19419-146] - BSA and Azide free (Capture) (ab242602)
Representative standard curve from corresponding SimpleStep ELISA® Kit (ab214573).
Downloads
Product protocols
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- Visit troubleshooting
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