AB221615

Anti-IPO11 antibody

Name: Anti-IPO11 antibody (ab221615) | Abcam
Brand: Abcam Limited
SKU: AB221615

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(3 Publications)

Rabbit Polyclonal IPO11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IPO11 aa 800 to C-terminus.

View Alternative Names

RANBP11, IPO11, Importin-11, Imp11, Ran-binding protein 11, RanBP11

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO11 antibody (AB221615)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO11 antibody (AB221615)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling IPO11 with ab221615 at 1/2500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-IPO11 antibody (AB221615)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IPO11 antibody (AB221615)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling IPO11 with ab221615 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IPO11 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UI26

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IPO11 also known as Importin-11 is a protein involved in nuclear transport. It weighs approximately 112 kDa and is expressed in multiple tissues including the brain lung and liver. It functions mechanically by interacting with nuclear localization signals on target proteins facilitating their transport across the nuclear envelope through the nuclear pore complex. This transport is dependent on the Ran GTPase cycle which provides directional movement by altering affinity for cargo proteins.

Biological function summary

IPO11 plays a role in the regulation of protein localization and gene expression. It is part of various nuclear transport complexes functioning to import key regulatory proteins like transcription factors and enzymes into the nucleus. This role supports cell cycle progression and cellular responses to external stimuli by ensuring that necessary proteins reach the nucleus for specific gene regulation activities.

Pathways

IPO11 is involved in the nuclear import pathway and the Ran GTPase cycle. These pathways are essential for the selective transport of proteins and RNAs between the nucleus and cytoplasm. IPO11 works closely with proteins like Ran and other importins to execute these processes. It ensures the transport is timely and consistently provided for the correct nuclear protein composition.

IPO11 has been associated with cancer and neurodegenerative diseases. Aberrant function or expression levels of IPO11 may lead to mislocalization of tumor suppressor proteins or oncogenes contributing to tumorigenesis. It also interacts with proteins involved in neurodegenerative pathways such as tau in Alzheimer's disease affecting its transport and function within neurons. This connection indicates potential involvement of IPO11 in disease progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBE2E3, and of RPL12 (By similarity).

See full target information IPO11

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational andrology and urology 12:176-186 PubMed36915888

2023

Identification and validation of immunohistochemical marker panels to predict the prognosis of muscle invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yidie Ying,Ziwei Wang,Yuxin Tan,Haotian Cao,Hongliang Gao,Zhensheng Zhang,Shuxiong Zeng,Chuanliang Xu

iScience 25:105115 PubMed36185355

2022

RPRM negatively regulates ATM levels through its nuclear translocation on irradiation mediated by CDK4/6 and IPO11.

Applications

Unspecified application

Species

Unspecified reactive species

Yarui Zhang,Guomin Ou,Zhujing Ye,Zhou Zhou,Qianlin Cao,Mengting Li,Jingdong Wang,Jianping Cao,Hongying Yang

Neoplasia (New York, N.Y.) 19:346-353 PubMed28319808

2017

Identifying Regulatory Posttranslational Modifications of PD-L1: A Focus on Monoubiquitinaton.

Applications

Unspecified application

Species

Unspecified reactive species

Henrick Horita,Andy Law,Soonjin Hong,Kim Middleton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IPO11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO11 antibody (AB221615)

Immunocytochemistry/ Immunofluorescence - Anti-IPO11 antibody (AB221615)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise