AB187175

Anti-IPO5 antibody

Name: Anti-IPO5 antibody (ab187175) | Abcam
Brand: Abcam Limited
SKU: AB187175

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(5 Publications)

Rabbit Polyclonal IPO5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IPO5 aa 1-300.

View Alternative Names

KPNB3, RANBP5, IPO5, Importin-5, Imp5, Importin subunit beta-3, Karyopherin beta-3, Ran-binding protein 5, RanBP5

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-IPO5 antibody (AB187175)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IPO5 antibody (AB187175)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187175. Blue DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling Karyopherin beta 3 with ab187175 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling Karyopherin beta 3 with ab187175 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling Karyopherin beta 3 with ab187175 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-IPO5 antibody (AB187175)

All lanes:

Western blot - Anti-IPO5 antibody (ab187175) at 1/500 dilution

Lane 1:

SW620 cell extract

Lane 2:

Hela cell extract

Lane 3:

293T cell extract

Lane 4:

HepG2 cell extract

Lane 5:

Mouse skeletal muscle extract

Lane 6:

Mouse testis extract

Lane 7:

Mouse heart extract

Lane 8:

Mouse brain extract

Lane 9:

Mouse ovary extract

Predicted band size: 124 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IPO5 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O00410

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.02% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target IPO5 also known as Importin-5 functions mechanically as a nuclear transport receptor. Importin-5 has a molecular mass of approximately 120 kDa. It operates within the importin-beta family and facilitates protein transport across the nuclear envelope by recognizing nuclear localization signals. IPO5 expresses in a variety of tissues with higher levels noted in areas such as the brain and testis.

Biological function summary

Understanding its role in cargo transport into the nucleus helps researchers comprehend cellular function and regulation. IPO5 forms part of a complex responsible for binding and importing nucleoplasmin-class cargoes establishing its significance in relaying proteins imperative for cell cycle progression and genome regulation. By shuttling these cargo proteins IPO5 maintains nuclear pore complex functionality and contributes to cellular dynamics.

Pathways

IPO5 serves an integral role in nucleocytoplasmic transport and the cell cycle. It exhibits interaction with the Ran GTPase pathway essential for the import and export cycle of proteins between the cytoplasm and nucleus. IPO5's activity is related to other importin-beta family proteins which are important for transporting proteins involved in transcription and other nuclear processes.

IPO5 shows connections to certain cancer forms and neurodegenerative diseases. Deregulation of IPO5 can influence tumorigenesis due to its involvement in controlling cell proliferation. Its association with proteins like TP53 indicates its relevance in tumor suppression pathways. Additionally alterations in IPO5 functionality have implications in neurodegeneration affecting proteins important for neuronal health and survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607, PubMed : 9687515). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity). In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.

See full target information IPO5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of immunopathology and pharmacology 39:3946320251318147 PubMed39936565

2025

Melatonin alleviates oral epithelial cell inflammation via Keap1/Nrf2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Zhang,Wenjing Wang,Rongxia Zhang,Yaxuan Liu,Yamei Wang,Yang Bai,Chencong Li

Current issues in molecular biology 46:3839-3865 PubMed38785507

2024

Prognostic Value of Histone Acetyl Transferase 1 (HAT-1) and Inflammatory Signatures in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Laura Jiménez-Álvarez,Oscar Fraile-Martinez,Cielo Garcia-Montero,Luis G Guijarro,Leonel Pekarek,Silvestra Barrena-Blázquez,Ángel Asúnsolo,Laura López-González,María Del Val Toledo-Lobo,Melchor Álvarez-Mon,Miguel A Saez,Alberto Gutiérrez-Calvo,Raúl Díaz-Pedrero

Journal of translational medicine 22:6 PubMed38167440

2024

TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Lin Hao,Yang-Fan Lv,De-Feng Li,Fu-Hai Bai,Ji Gong,Guang-Qiang Pan,Lin-Xi Su,Ya-Li Wang,Wan-Lei Fu,Bo Liu,Lu Huang,Dong Yan,Qiu-Lin Tan,Jin-Yi Liu,Qiao-Nan Guo

Journal of virology 96:e0152222 PubMed36409110

2022

Porcine Circovirus Type 2 Hijacks Host IPO5 to Sustain the Intracytoplasmic Stability of Its Capsid Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Cui Lin,Jingyu Hu,Yadong Dai,Huandong Zhang,Kang Xu,Weiren Dong,Yan Yan,Xiran Peng,Jiyong Zhou,Jinyan Gu

Journal of cell science 133: PubMed31340999

2019

DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Shaozu Fu,Zhengqian Wu,Wenping Yang,Yi Ru,Hongbing Shu,Xiangtao Liu,Haixue Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IPO5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-IPO5 antibody (AB187175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPO5 antibody (AB187175)

Western blot - Anti-IPO5 antibody (AB187175)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise