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AB88695

Anti-IPO5 antibody

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(2 Publications)

Mouse Polyclonal IPO5 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IPO5.

View Alternative Names

KPNB3, RANBP5, IPO5, Importin-5, Imp5, Importin subunit beta-3, Karyopherin beta-3, Ran-binding protein 5, RanBP5

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IPO5 antibody (AB88695)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IPO5 antibody (AB88695)

ab88695, at 10 μg/ml, staining Karyopherin (importin) beta 3 in HeLa cell by immunofluorescence.

Western blot - Anti-IPO5 antibody (AB88695)
  • WB

Unknown

Western blot - Anti-IPO5 antibody (AB88695)

All lanes:

Western blot - Anti-IPO5 antibody (ab88695) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 124 kDa

Observed band size: 110 kDa

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Western blot - Anti-IPO5 antibody (AB88695)
  • WB

Unknown

Western blot - Anti-IPO5 antibody (AB88695)

All lanes:

Western blot - Anti-IPO5 antibody (ab88695) at 1 µg/mL

All lanes:

rat brain tissue lysate at 50 µg

Predicted band size: 124 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IPO5.

O00410

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target IPO5 also known as Importin-5 functions mechanically as a nuclear transport receptor. Importin-5 has a molecular mass of approximately 120 kDa. It operates within the importin-beta family and facilitates protein transport across the nuclear envelope by recognizing nuclear localization signals. IPO5 expresses in a variety of tissues with higher levels noted in areas such as the brain and testis.
Biological function summary

Understanding its role in cargo transport into the nucleus helps researchers comprehend cellular function and regulation. IPO5 forms part of a complex responsible for binding and importing nucleoplasmin-class cargoes establishing its significance in relaying proteins imperative for cell cycle progression and genome regulation. By shuttling these cargo proteins IPO5 maintains nuclear pore complex functionality and contributes to cellular dynamics.

Pathways

IPO5 serves an integral role in nucleocytoplasmic transport and the cell cycle. It exhibits interaction with the Ran GTPase pathway essential for the import and export cycle of proteins between the cytoplasm and nucleus. IPO5's activity is related to other importin-beta family proteins which are important for transporting proteins involved in transcription and other nuclear processes.

IPO5 shows connections to certain cancer forms and neurodegenerative diseases. Deregulation of IPO5 can influence tumorigenesis due to its involvement in controlling cell proliferation. Its association with proteins like TP53 indicates its relevance in tumor suppression pathways. Additionally alterations in IPO5 functionality have implications in neurodegeneration affecting proteins important for neuronal health and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5 (PubMed : 11682607, PubMed : 9687515). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity). In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
See full target information IPO5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36066346

2022

A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3.

Applications

Unspecified application

Species

Unspecified reactive species

Alonso Javier Pardal,Andrew James Bowman

Medical science monitor : international medical jo 24:1424-1433 PubMed29519997

2018

Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Liu,Bingrong Liu
View all publications
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Product promise

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