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AB236772

Anti-IPP antibody

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(1 Publication)

Rabbit Polyclonal IPP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IPP aa 400-550.

View Alternative Names

KLHL27, IPP, Actin-binding protein IPP, Intracisternal A particle-promoted polypeptide, Kelch-like protein 27

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody (AB236772)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPP antibody (AB236772)

Paraffin-embedded human kidney tissue stained for IPP with ab236772 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-IPP antibody (AB236772)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IPP antibody (AB236772)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for IPP using ab236772 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-IPP antibody (AB236772)
  • WB

Supplier Data

Western blot - Anti-IPP antibody (AB236772)

All lanes:

Western blot - Anti-IPP antibody (ab236772) at 1/500 dilution

All lanes:

Rat kidney tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human IPP aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

Q9Y573

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The molecule isopentenyl pyrophosphate (IPP) sometimes referred to as isopentenyl diphosphate serves as an important intermediate in the biosynthesis of terpenes and isoprenoids. IPP a small molecule with a molecular mass of 246.1 g/mol participates in various enzymatic reactions within the mevalonate pathway. This pathway occurs in the cytosol of eukaryotic cells and the peroxisomes in plants. In terms of its expression while IPP is not a protein or gene with specific expression levels the enzymes related to its synthesis and utilization show varying activity across different tissues.
Biological function summary

IPP functions as a precursor for various essential isoprenoid compounds encompassing sterols dolichols and ubiquinones. It acts within a network of enzymes converting basic building blocks into complex molecules that maintain cell membranes and facilitate electron transport. These reactions often involve enzyme complexes like synthases that regulate the flow of intermediates. Moreover IPP plays a substantial role in the synthesis of other molecules like cholesterol and steroid hormones reflecting its broad biological influence.

Pathways

IPP engages prominently in the mevalonate and the methylerythritol phosphate (MEP) pathways. The mevalonate pathway produces isoprenoids which are vital for cell growth and development. Within these pathways IPP can interconvert with dimethylallyl pyrophosphate (DMAPP) another critical molecule. Enzymes like HMG-CoA reductase and isopentenyl-diphosphate isomerase control these reactions linking IPP to key biological pathways involved in metabolic regulation and cellular differentiation.

IPP's significance links to disorders related to cholesterol metabolism and immune system dysregulation. Defects in the mevalonate pathway associate with diseases like mevalonate kinase deficiency influencing levels of inflammatory proteins. Moreover IPP accumulation can activate immune cells through the recognition by Vγ9/VΔ2 T-cells implicating it in conditions such as autoimmunity or inflammation. This connection highlights the consequence of IPP in understanding metabolic and immune-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in organizing the actin cytoskeleton.
See full target information IPP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:991-1000 PubMed37994395

2023

ASIC1a contributes to the epithelial-mesenchymal transformation of breast cancer by activating the Ca /β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Wang,Chao Yang,Ruihua Yu,Ming Zhuang,Feng Jiang
View all publications
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