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AB196781

Anti-IPPK/IPK1 antibody

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(1 Publication)

Rabbit Polyclonal IPPK/IPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IPPK.

View Alternative Names

C9orf12, IPPK, Inositol-pentakisphosphate 2-kinase, IPK1 homolog, InsP5 2-kinase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IPPK/IPK1 antibody (AB196781)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IPPK/IPK1 antibody (AB196781)

Immunofluorescent analysis of HepG2 cells labeling IPPK/IPK1 with ab196781 at 1/100 dilution. The image on the right is pre-incubated with synthesized peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPPK/IPK1 antibody (AB196781)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IPPK/IPK1 antibody (AB196781)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling IPPK/IPK1 with ab196781 at 1/50 dilution. The image on the right is pre-incubated with synthesized peptide.

Western blot - Anti-IPPK/IPK1 antibody (AB196781)
  • WB

Supplier Data

Western blot - Anti-IPPK/IPK1 antibody (AB196781)

All lanes:

Western blot - Anti-IPPK/IPK1 antibody (ab196781) at 1/500 dilution

Lane 1:

RAW cell extracts

Lane 2:

K562 cell extracts

Lane 3:

LOVO cell extracts

Lane 4:

3T3 cell extract

Lane 5:

Cell extracts with synthesized peptide

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human IPPK. The exact immunogen used to generate this antibody is proprietary information.

Q9H8X2

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Alternative Products

Primary Antibodies

AB269959

Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

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Primary Antibodies

AB230807

Anti-IPPK/IPK1 antibody [EPR20893-54]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB194800

Anti-ZAP70 (phospho Y493) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 (phospho Y493) antibody (AB194800)

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Cell Lines & Lysates

AB267287

Human IPPK (IPK1) knockout HEK-293T cell line

Sanger Sequencing - Human IPPK (IPK1) knockout HEK-293T cell line (AB267287)

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Cell Lines & Lysates

AB258925

Human IPPK (IPK1) knockout HEK-293T cell lysate

Sanger Sequencing - Human IPPK (IPK1) knockout HEK-293T cell lysate (AB258925)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Inositol (13456)-pentakisphosphate 2-kinase commonly known as IPPK or IPK1 plays an important role in the phosphorylation of inositol phosphates. IPPK catalyzes the conversion of inositol pentakisphosphate (IP5) to inositol hexakisphosphate (IP6). This protein has a molecular mass of around 44 kDa. It is prominently expressed in the brain kidney and pancreas suggesting a broad importance across different tissues.
Biological function summary

IPPK contributes to the regulation of inositol phosphate metabolism. It does not act in isolation but as part of a network involving inositol polyphosphate kinases and phosphatases that help maintain cellular processes such as signal transduction and ion homeostasis. Although IPPK doesn't form part of a larger complex its activity impacts various cell signaling pathways by altering the levels of its substrate and product.

Pathways

IPPK participates significantly in the phosphoinositide signaling pathway impacting processes such as cellular growth survival and apoptosis. This pathway also involves PI3Ks and PTEN which are critical in signal transduction. Moreover IPPK indirectly influences the synthesis of IP7 regulated by inositol hexakisphosphate kinases integrating its function into broader metabolic pathways.

IPPK has associations with pathologies like cancer and metabolic disorders. Abnormal activity or expression levels of IPPK can alter cellular signaling pathways which might lead to tumorigenesis or metabolic syndrome. It closely interacts with proteins such as mTOR highlighting its role in cancer pathways and PI3K/AKT pathway dysregulation common in metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-induced apoptosis.
See full target information IPPK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 7:1285-306 PubMed26286618

2015

Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Frances-Rose Schumacher,Keith Siew,Jinwei Zhang,Clare Johnson,Nicola Wood,Sarah E Cleary,Raya S Al Maskari,James T Ferryman,Iris Hardege, Yasmin,Nichola L Figg,Radoslav Enchev,Axel Knebel,Kevin M O'Shaughnessy,Thimo Kurz
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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