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AB230807

Anti-IPPK/IPK1 antibody [EPR20893-54]

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(1 Publication)

Rabbit Recombinant Monoclonal IPPK/IPK1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C9orf12, IPPK, Inositol-pentakisphosphate 2-kinase, IPK1 homolog, InsP5 2-kinase

5 Images
Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling IPPK/IPK1 with ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (Human embryonic kidney epithelial cell) cells labelling IPPK/IPK1 with ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)
  • IP

Unknown

Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)

IPPK/IPK1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab230807 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab230807 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2 : ab230807 IP in MCF7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230807 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807)

Predicted band size: 56 kDa

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Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)
  • WB

Lab

Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 26 seconds

All lanes:

Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)
  • WB

Lab

Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (AB230807)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were kindly provided by collaborator.

ab230807 was shown to specifically react with IPPK/IPK1 in wild-type HEK-293 cells as signal was lost in IPPK/IPK1 knockout cells. Wild-type and IPPK/IPK1 knockout samples were subjected to SDS-PAGE. ab230807 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

Exposure time : 37 seconds

All lanes:

Western blot - Anti-IPPK/IPK1 antibody [EPR20893-54] (ab230807) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

IPPK/IPK1 knock out HEK-293 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

  • Carrier free

    Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20893-54

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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AB196781

Anti-IPPK/IPK1 antibody

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Inositol (13456)-pentakisphosphate 2-kinase commonly known as IPPK or IPK1 plays an important role in the phosphorylation of inositol phosphates. IPPK catalyzes the conversion of inositol pentakisphosphate (IP5) to inositol hexakisphosphate (IP6). This protein has a molecular mass of around 44 kDa. It is prominently expressed in the brain kidney and pancreas suggesting a broad importance across different tissues.
Biological function summary

IPPK contributes to the regulation of inositol phosphate metabolism. It does not act in isolation but as part of a network involving inositol polyphosphate kinases and phosphatases that help maintain cellular processes such as signal transduction and ion homeostasis. Although IPPK doesn't form part of a larger complex its activity impacts various cell signaling pathways by altering the levels of its substrate and product.

Pathways

IPPK participates significantly in the phosphoinositide signaling pathway impacting processes such as cellular growth survival and apoptosis. This pathway also involves PI3Ks and PTEN which are critical in signal transduction. Moreover IPPK indirectly influences the synthesis of IP7 regulated by inositol hexakisphosphate kinases integrating its function into broader metabolic pathways.

IPPK has associations with pathologies like cancer and metabolic disorders. Abnormal activity or expression levels of IPPK can alter cellular signaling pathways which might lead to tumorigenesis or metabolic syndrome. It closely interacts with proteins such as mTOR highlighting its role in cancer pathways and PI3K/AKT pathway dysregulation common in metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-induced apoptosis.
See full target information IPPK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2416982121 PubMed39602251

2024

inositol hexaphosphate pathways couple to RNA interference and pathogen defense.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Xu,Yifan Sun,Peter Breen,Gary Ruvkun,Kai Mao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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