Rabbit Recombinant Monoclonal IPPK/IPK1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959), expandable thumbnail](https://content.abcam.com/products/images/ippk-ipk1-antibody-epr20893-54-bsa-and-azide-free-ab269959--immunoprecipitation-img4401.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)")
![Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959), expandable thumbnail](https://content.abcam.com/products/images/ippk-ipk1-antibody-epr20893-54-bsa-and-azide-free-ab269959--flow-cytometry-intracellular-img26390.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)")
![Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959), expandable thumbnail](https://content.abcam.com/products/images/ippk-ipk1-antibody-epr20893-54-bsa-and-azide-free-ab269959--flow-cytometry-intracellular-img2775.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | ICC | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-alpha-induced apoptosis.
Alternative names
Inositol-pentakisphosphate 2-kinase, IPK1 homolog, InsP5 2-kinase, IPPK, C9orf12
Recommended products
Rabbit Recombinant Monoclonal IPPK/IPK1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Inositol-pentakisphosphate 2-kinase, IPK1 homolog, InsP5 2-kinase, IPPK, C9orf12
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20893-54
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab269959 is the carrier-free version of Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Inositol (13456)-pentakisphosphate 2-kinase commonly known as IPPK or IPK1 plays an important role in the phosphorylation of inositol phosphates. IPPK catalyzes the conversion of inositol pentakisphosphate (IP5) to inositol hexakisphosphate (IP6). This protein has a molecular mass of around 44 kDa. It is prominently expressed in the brain kidney and pancreas suggesting a broad importance across different tissues.
- Biological function summary
IPPK contributes to the regulation of inositol phosphate metabolism. It does not act in isolation but as part of a network involving inositol polyphosphate kinases and phosphatases that help maintain cellular processes such as signal transduction and ion homeostasis. Although IPPK doesn't form part of a larger complex its activity impacts various cell signaling pathways by altering the levels of its substrate and product.
- Pathways
IPPK participates significantly in the phosphoinositide signaling pathway impacting processes such as cellular growth survival and apoptosis. This pathway also involves PI3Ks and PTEN which are critical in signal transduction. Moreover IPPK indirectly influences the synthesis of IP7 regulated by inositol hexakisphosphate kinases integrating its function into broader metabolic pathways.
- Associated diseases and disorders
IPPK has associations with pathologies like cancer and metabolic disorders. Abnormal activity or expression levels of IPPK can alter cellular signaling pathways which might lead to tumorigenesis or metabolic syndrome. It closely interacts with proteins such as mTOR highlighting its role in cancer pathways and PI3K/AKT pathway dysregulation common in metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959), expandable thumbnail](https://content.abcam.com/products/images/ippk-ipk1-antibody-epr20893-54-bsa-and-azide-free-ab269959--immunoprecipitation-img4401.jpg "Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959)")
Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959)
IPPK/IPK1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug with Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807 IP in MCF7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807).
All lanes: Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807)
Predicted band size: 56 kDa
![Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959), expandable thumbnail](https://content.abcam.com/products/images/ippk-ipk1-antibody-epr20893-54-bsa-and-azide-free-ab269959--flow-cytometry-intracellular-img26390.jpg "Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959)")
Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labelling IPPK/IPK1 with Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807).
![Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959), expandable thumbnail](https://content.abcam.com/products/images/ippk-ipk1-antibody-epr20893-54-bsa-and-azide-free-ab269959--flow-cytometry-intracellular-img2775.jpg "Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959)")
Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (ab269959)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling IPPK/IPK1 with Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IPPK/IPK1 antibody [EPR20893-54] ab230807).
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