AB269959

Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IPPK/IPK1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

C9orf12, IPPK, Inositol-pentakisphosphate 2-kinase, IPK1 homolog, InsP5 2-kinase

3 Images

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling IPPK/IPK1 with ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230807).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labelling IPPK/IPK1 with ab230807 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230807).

Unknown

Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

IPPK/IPK1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug with ab230807 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab230807 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2 : ab230807 IP in MCF7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230807 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230807).

All lanes:

Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] (<a href='/en-us/products/primary-antibodies/ippk-ipk1-antibody-epr20893-54-ab230807'>ab230807</a>)

Predicted band size: 56 kDa

false

Unconjugated

Anti-IPPK/IPK1 antibody [EPR20893-54]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20893-54

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab269959 is the carrier-free version of ab230807.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Inositol (13456)-pentakisphosphate 2-kinase commonly known as IPPK or IPK1 plays an important role in the phosphorylation of inositol phosphates. IPPK catalyzes the conversion of inositol pentakisphosphate (IP5) to inositol hexakisphosphate (IP6). This protein has a molecular mass of around 44 kDa. It is prominently expressed in the brain kidney and pancreas suggesting a broad importance across different tissues.

Biological function summary

IPPK contributes to the regulation of inositol phosphate metabolism. It does not act in isolation but as part of a network involving inositol polyphosphate kinases and phosphatases that help maintain cellular processes such as signal transduction and ion homeostasis. Although IPPK doesn't form part of a larger complex its activity impacts various cell signaling pathways by altering the levels of its substrate and product.

Pathways

IPPK participates significantly in the phosphoinositide signaling pathway impacting processes such as cellular growth survival and apoptosis. This pathway also involves PI3Ks and PTEN which are critical in signal transduction. Moreover IPPK indirectly influences the synthesis of IP7 regulated by inositol hexakisphosphate kinases integrating its function into broader metabolic pathways.

IPPK has associations with pathologies like cancer and metabolic disorders. Abnormal activity or expression levels of IPPK can alter cellular signaling pathways which might lead to tumorigenesis or metabolic syndrome. It closely interacts with proteins such as mTOR highlighting its role in cancer pathways and PI3K/AKT pathway dysregulation common in metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion channel regulation. It also protects cells from TNF-induced apoptosis.

See full target information IPPK

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

Flow Cytometry (Intracellular) - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

Immunoprecipitation - Anti-IPPK/IPK1 antibody [EPR20893-54] - BSA and Azide free (AB269959)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise