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AB235372

Anti-IPT antibody

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(1 Publication)

Rabbit Polyclonal IPT antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIT1.

View Alternative Names

IPT, MOD5, TRIT1, tRNA dimethylallyltransferase, Isopentenyl-diphosphate:tRNA isopentenyltransferase, hGRO1, tRNA isopentenyltransferase 1, IPP transferase, IPPT, IPTase

1 Images
Western blot - Anti-IPT antibody (AB235372)
  • WB

Supplier Data

Western blot - Anti-IPT antibody (AB235372)

All lanes:

Western blot - Anti-IPT antibody (ab235372) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRIT1.

Q9H3H1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target 'IPT' also known as isopentenyl transferase is an enzyme involved in the catalysis of certain biochemical reactions. This enzyme has a molecular mass of approximately 32 kDa and is often expressed in various plant tissues including roots shoots and leaves. In the context of its role IPT facilitates the transfer of the isopentenyl group to adenine leading to the biosynthesis of cytokinins which are essential hormones for plant growth and development.
Biological function summary

IPT plays a central role in cytokinin biosynthesis by converting dimethylallyl diphosphate (DMAPP) and adenosine monophosphate (AMP) into isopentenyl adenine a precursor of cytokinin. Cytokinins together with auxins regulate cell division differentiation and apical dominance. IPT is often found as a part of a complex network of enzymatic reactions contributing to these processes. Its activity profoundly affects plant morphology and physiology by influencing the allocation of resources and determining plant architecture.

Pathways

IPT fits into the cytokinin biosynthetic pathway collaborating with other enzymes involved in plant hormone regulation. It plays an important role in modulating the balance between cytokinin and auxin levels which controls many developmental processes. The enzyme is closely associated with the IPT family of proteins and interfaces with the homodomain transcription factors that regulate plant growth responses to environmental stimuli. This places IPT in pivotal intersection points of hormonal and stress response pathways in plants.

Research highlights the importance of IPT's manipulation in plant biotechnology for crop improvement and stress resistance. Its association with increased cytokinin production has shown benefits such as delayed leaf senescence and enhanced resistance to drought and pathogens. There are indirect links between IPT and proteins involved in disease resistance pathways such as the pathogenesis-related proteins. Exploiting IPT's function in agricultural settings can lead to better yield stability and reduced agricultural losses due to environmental stressors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A37) (PubMed : 11111046, PubMed : 24126054, PubMed : 24901367, PubMed : 34774131). Mediates modification of a limited subset of tRNAs : tRNA(Ser)(AGA), tRNA(Ser)(CGA), tRNA(Ser)(UGA), as well as partial modification of the selenocysteine tRNA(Ser)(UCA) (PubMed : 24126054). TRIT1 is therefore required for selenoprotein expression (PubMed : 24126054).
See full target information TRIT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eado3141 PubMed39047111

2024

Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwei Hu,Rui Li,Dongxu Gong,Pei Hu,Jitu Xu,Yingjie Ai,Xiaojie Zhao,Chencheng Hu,Minghuan Xu,Chenxi Liu,Shuyu Chen,Jie Fan,Zhonghua Zhao,Zhigang Zhang,Huijuan Wu,Yanyong Xu
View all publications
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