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AB249810

Anti-IPT antibody [EPR12517] - BSA and Azide free

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Rabbit Recombinant Monoclonal IPT antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

IPT, MOD5, TRIT1, tRNA dimethylallyltransferase, Isopentenyl-diphosphate:tRNA isopentenyltransferase, hGRO1, tRNA isopentenyltransferase 1, IPP transferase, IPPT, IPTase

2 Images
Flow Cytometry (Intracellular) - Anti-IPT antibody [EPR12517] - BSA and Azide free (AB249810)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IPT antibody [EPR12517] - BSA and Azide free (AB249810)

This data was developed using ab174308, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Raji cells labeling IPT with ab174308 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

Western blot - Anti-IPT antibody [EPR12517] - BSA and Azide free (AB249810)
  • WB

Supplier Data

Western blot - Anti-IPT antibody [EPR12517] - BSA and Azide free (AB249810)

This data was developed using ab174308, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-IPT antibody [EPR12517] (<a href='/en-us/products/primary-antibodies/ipt-antibody-epr12517-ab174308'>ab174308</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Fetal lung lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Predicted band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12517

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249810 is the carrier-free version of ab174308.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target 'IPT' also known as isopentenyl transferase is an enzyme involved in the catalysis of certain biochemical reactions. This enzyme has a molecular mass of approximately 32 kDa and is often expressed in various plant tissues including roots shoots and leaves. In the context of its role IPT facilitates the transfer of the isopentenyl group to adenine leading to the biosynthesis of cytokinins which are essential hormones for plant growth and development.
Biological function summary

IPT plays a central role in cytokinin biosynthesis by converting dimethylallyl diphosphate (DMAPP) and adenosine monophosphate (AMP) into isopentenyl adenine a precursor of cytokinin. Cytokinins together with auxins regulate cell division differentiation and apical dominance. IPT is often found as a part of a complex network of enzymatic reactions contributing to these processes. Its activity profoundly affects plant morphology and physiology by influencing the allocation of resources and determining plant architecture.

Pathways

IPT fits into the cytokinin biosynthetic pathway collaborating with other enzymes involved in plant hormone regulation. It plays an important role in modulating the balance between cytokinin and auxin levels which controls many developmental processes. The enzyme is closely associated with the IPT family of proteins and interfaces with the homodomain transcription factors that regulate plant growth responses to environmental stimuli. This places IPT in pivotal intersection points of hormonal and stress response pathways in plants.

Research highlights the importance of IPT's manipulation in plant biotechnology for crop improvement and stress resistance. Its association with increased cytokinin production has shown benefits such as delayed leaf senescence and enhanced resistance to drought and pathogens. There are indirect links between IPT and proteins involved in disease resistance pathways such as the pathogenesis-related proteins. Exploiting IPT's function in agricultural settings can lead to better yield stability and reduced agricultural losses due to environmental stressors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A37) (PubMed : 11111046, PubMed : 24126054, PubMed : 24901367, PubMed : 34774131). Mediates modification of a limited subset of tRNAs : tRNA(Ser)(AGA), tRNA(Ser)(CGA), tRNA(Ser)(UGA), as well as partial modification of the selenocysteine tRNA(Ser)(UCA) (PubMed : 24126054). TRIT1 is therefore required for selenoprotein expression (PubMed : 24126054).
See full target information TRIT1
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Product promise

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For full details, please see our Terms & Conditions

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