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AB241197

Anti-IQ motif containing B1 antibody

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(1 Publication)

Rabbit Polyclonal IQ motif containing B1 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IQCB1 aa 500 to C-terminus.

View Alternative Names

KIAA0036, NPHP5, OK/SW-cl.85, IQCB1, IQ calmodulin-binding motif-containing protein 1, Nephrocystin-5, p53 and DNA damage-regulated IQ motif protein, PIQ

1 Images
Immunoprecipitation - Anti-IQ motif containing B1 antibody (AB241197)
  • IP

Supplier Data

Immunoprecipitation - Anti-IQ motif containing B1 antibody (AB241197)

IQ motif containing B1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241197 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241197 at 1 μg/ml.

Lane 1 : ab241197 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-IQ motif containing B1 antibody (ab241197)

Predicted band size: 69 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human IQCB1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15051

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

ab241197 has not performed satisfactorily when used for WB of IQ motif containing B1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of IQ motif containing B1.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to IQ motif containing B1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IQ motif containing B1 also known as IQCB1 or NPHP5 is a protein that functions mechanically as a part of cellular pathways involving microtubule dynamics. This protein has an approximate mass of 69 kDa and is widely expressed in various tissues with higher levels noted in the retina and kidney. The function of IQCB1 is closely related to its role in intracellular transport and sensory cilia signaling.
Biological function summary

IQ motif containing B1 plays an important role in the ciliary transition zone which is an important component in cilia formation and function. This protein is part of a protein complex known as the nephronophthisis module where it collaborates with other proteins to maintain ciliary structural integrity. Defects or mutations in IQCB1 may disrupt this module highlighting its significance in normal cellular operations.

Pathways

The IQ motif containing B1 is involved in the ciliogenesis and cilia function pathways. In these pathways it interacts with proteins such as NPHP6 (also known as CEP290) to regulate ciliary assembly and ensure proper signal transduction. IQCB1's function is essential for the proper maintenance of these pathways which are critical for sensory perception and cellular communication.

Mutations in IQ motif containing B1 are linked to conditions such as nephronophthisis and retinitis pigmentosa. The protein's interaction with other proteins like RPGR which is associated with retinitis pigmentosa emphasizes its role in multi-systemic diseases affecting the kidneys and eyes. Understanding the function and interaction of IQCB1 with these proteins provides insights into the mechanisms underlying these disorders and may guide future therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in ciliogenesis. The function in an early step in cilia formation depends on its association with CEP290/NPHP6 (PubMed : 21565611, PubMed : 23446637). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2 and BBS5 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating CEP290/NPHP6 (PubMed : 25552655).
See full target information IQCB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:8825784 PubMed35281474

2022

BMSC-EV-derived lncRNA NORAD Facilitates Migration, Invasion, and Angiogenesis in Osteosarcoma Cells by Regulating CREBBP Delivery of miR-877-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Feng,Zhengwei Li,Liang Yang,Haidong Liang,Hongtao He,Lin Liu,Wei Zhang
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Product promise

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