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AB69927

Anti-IQ motif containing B1 antibody

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(2 Publications)

Mouse Polyclonal IQ motif containing B1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IQCB1.

View Alternative Names

KIAA0036, NPHP5, OK/SW-cl.85, IQCB1, IQ calmodulin-binding motif-containing protein 1, Nephrocystin-5, p53 and DNA damage-regulated IQ motif protein, PIQ

1 Images
Western blot - Anti-IQ motif containing B1 antibody (AB69927)
  • WB

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Western blot - Anti-IQ motif containing B1 antibody (AB69927)

All lanes:

Western blot - Anti-IQ motif containing B1 antibody (ab69927) at 1/500 dilution

Lane 1:

IQ motif containing B1 transfected 293T cell lysate at 25 µg

Lane 2:

non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IQCB1.

Q15051

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p>Predicted molecular weight for isoform 2: 54 kDa.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IQ motif containing B1 also known as IQCB1 or NPHP5 is a protein that functions mechanically as a part of cellular pathways involving microtubule dynamics. This protein has an approximate mass of 69 kDa and is widely expressed in various tissues with higher levels noted in the retina and kidney. The function of IQCB1 is closely related to its role in intracellular transport and sensory cilia signaling.
Biological function summary

IQ motif containing B1 plays an important role in the ciliary transition zone which is an important component in cilia formation and function. This protein is part of a protein complex known as the nephronophthisis module where it collaborates with other proteins to maintain ciliary structural integrity. Defects or mutations in IQCB1 may disrupt this module highlighting its significance in normal cellular operations.

Pathways

The IQ motif containing B1 is involved in the ciliogenesis and cilia function pathways. In these pathways it interacts with proteins such as NPHP6 (also known as CEP290) to regulate ciliary assembly and ensure proper signal transduction. IQCB1's function is essential for the proper maintenance of these pathways which are critical for sensory perception and cellular communication.

Mutations in IQ motif containing B1 are linked to conditions such as nephronophthisis and retinitis pigmentosa. The protein's interaction with other proteins like RPGR which is associated with retinitis pigmentosa emphasizes its role in multi-systemic diseases affecting the kidneys and eyes. Understanding the function and interaction of IQCB1 with these proteins provides insights into the mechanisms underlying these disorders and may guide future therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in ciliogenesis. The function in an early step in cilia formation depends on its association with CEP290/NPHP6 (PubMed : 21565611, PubMed : 23446637). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2 and BBS5 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating CEP290/NPHP6 (PubMed : 25552655).
See full target information IQCB1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 77:195-212 PubMed31177295

2019

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Delowar Hossain,Marine Barbelanne,William Y Tsang

Human molecular genetics 23:40-51 PubMed23943788

2013

BBS mutations modify phenotypic expression of CEP290-related ciliopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Seongjin Seo,Sajag Bhattarai,Kevin Bugge,Charles C Searby,Qihong Zhang,Arlene V Drack,Edwin M Stone,Val C Sheffield
View all publications
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