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AB223041

Anti-IQCG antibody

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(1 Publication)

Rabbit Polyclonal IQCG antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IQCG aa 1-150.

View Alternative Names

IQCG, Dynein regulatory complex protein 9, IQ domain-containing protein G

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IQCG antibody (AB223041)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IQCG antibody (AB223041)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IQCG (green) using ab223041 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Western blot - Anti-IQCG antibody (AB223041)
  • WB

Supplier Data

Western blot - Anti-IQCG antibody (AB223041)

All lanes:

Western blot - Anti-IQCG antibody (ab223041) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse skeletal muscle tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 52 kDa

Observed band size: 41 kDa,52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human IQCG aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9H095

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IQ Domain-Containing G (IQCG) is a protein known for its role in intracellular signaling. It presents alternative names including IQ motif-containing protein G. Its molecular weight is approximately 75 kDa. IQCG expresses mainly in testicular tissue suggesting a specialized function in reproductive biology. The protein contains conserved IQ motifs which indicate its ability to bind calmodulin and potentially influence calcium signaling related activities.
Biological function summary

The processes involving IQCG impact regulation of spermatogenesis an important reproductive process. IQCG is part of a larger multi-protein complex that contributes to centrosome and cilium formation. By interacting with calmodulin it may modulate cellular responses to calcium fluctuations that are necessary for male germ cell division and differentiation.

Pathways

IQCG integrates into the cilium assembly pathway and the calcium signaling pathway. These integrative roles suggest IQCG may collaborate with proteins such as centrin and calmodulin facilitating the proper assembly and function of cilia. Such interactions are critical for cellular processes like movement and cell cycle regulation highlighting IQCG's function in specialized cellular contexts.

Altered IQCG function associates with male infertility and some ciliopathies. Male infertility might link to disturbed spermatogenesis due to improper centrosome or cilium assembly. Additionally proteins such as centrin along with IQCG suggest possible mechanistic pathways of pathology within the context of ciliopathies where altered cellular structures contribute to disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Binds calmodulin when cellular Ca(2+) levels are low and thereby contributes to the regulation of calcium and calmodulin-dependent protein kinase IV (CAMK4) activity; contributes to the regulation of CAMK4 signaling cascades. Required for normal axoneme assembly in sperm flagella, normal sperm tail formation and for male fertility.
See full target information IQCG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 324:L325-L334 PubMed36719084

2023

Hyperoxia impairs intraflagellar transport and causes dysregulated metabolism with resultant decreased cilia length.

Applications

Unspecified application

Species

Unspecified reactive species

Daniella Teape,Abigail Peterson,Nagib Ahsan,Kimberlyn Ellis,Nicholas Correia,Ryan Luo,Katy Hegarty,Hongwei Yao,Phyllis Dennery
View all publications
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Product promise

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