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Rabbit Polyclonal IQCG antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IQCG aa 1-150.

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Images

Western blot - Anti-IQCG antibody (AB223041), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IQCG antibody (AB223041), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human IQCG aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H095

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/200
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes. Binds calmodulin when cellular Ca(2+) levels are low and thereby contributes to the regulation of calcium and calmodulin-dependent protein kinase IV (CAMK4) activity; contributes to the regulation of CAMK4 signaling cascades. Required for normal axoneme assembly in sperm flagella, normal sperm tail formation and for male fertility.

Alternative names

DRC9, IQCG, Dynein regulatory complex protein 9, IQ domain-containing protein G

Recommended products

Rabbit Polyclonal IQCG antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IQCG aa 1-150.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human IQCG aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H095
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purity >95%

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IQ Domain-Containing G (IQCG) is a protein known for its role in intracellular signaling. It presents alternative names including IQ motif-containing protein G. Its molecular weight is approximately 75 kDa. IQCG expresses mainly in testicular tissue suggesting a specialized function in reproductive biology. The protein contains conserved IQ motifs which indicate its ability to bind calmodulin and potentially influence calcium signaling related activities.

Biological function summary

The processes involving IQCG impact regulation of spermatogenesis an important reproductive process. IQCG is part of a larger multi-protein complex that contributes to centrosome and cilium formation. By interacting with calmodulin it may modulate cellular responses to calcium fluctuations that are necessary for male germ cell division and differentiation.

Pathways

IQCG integrates into the cilium assembly pathway and the calcium signaling pathway. These integrative roles suggest IQCG may collaborate with proteins such as centrin and calmodulin facilitating the proper assembly and function of cilia. Such interactions are critical for cellular processes like movement and cell cycle regulation highlighting IQCG's function in specialized cellular contexts.

Associated diseases and disorders

Altered IQCG function associates with male infertility and some ciliopathies. Male infertility might link to disturbed spermatogenesis due to improper centrosome or cilium assembly. Additionally proteins such as centrin along with IQCG suggest possible mechanistic pathways of pathology within the context of ciliopathies where altered cellular structures contribute to disease development.

Product promise

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2 product images

  • Western blot - Anti-IQCG antibody (ab223041), expandable thumbnail

    Western blot - Anti-IQCG antibody (ab223041)

    All lanes: Western blot - Anti-IQCG antibody (ab223041) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate

    Lane 2: Mouse skeletal muscle tissue lysate

    Secondary

    All lanes: Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa

    Observed band size: 41 kDa, 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IQCG antibody (ab223041), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IQCG antibody (ab223041)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IQCG (green) using ab223041 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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