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Rabbit Monoclonal IQGAP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 12 publications.

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Images

Western blot - Anti-IQGAP1 antibody [EPR5220] (AB133490), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] (AB133490), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] (AB133490), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] (AB133490), expandable thumbnail
  • Western blot - Anti-IQGAP1 antibody [EPR5220] (AB133490), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Expected
Not recommended
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth (PubMed:15695813). May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed:20883816).

Alternative names

KIAA0051, IQGAP1, Ras GTPase-activating-like protein IQGAP1, p195

Recommended products

Rabbit Monoclonal IQGAP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5220
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IQGAP1 also known as IQ motif containing GTPase activating protein 1 acts as a scaffold protein that interacts with several signaling molecules. It weighs approximately 189 kDa. This protein is expressed in various tissues but shows high expression in the brain liver and kidney. IQGAP1 contains multiple domains including four IQ motifs that bind to calmodulin and a Rho GTPase-activating protein-related domain that mediates interactions with GTPases.

Biological function summary

IQGAP1 plays a critical role in cytoskeletal organization cell adhesion and intracellular signaling. It participates in the formation of multiprotein complexes facilitating communication and structural integrity within the cell. IQGAP1 stabilizes the actin cytoskeleton and affects cell motility and polarity. It interacts with proteins such as E-cadherin and beta-catenin affecting cell-cell adhesion and signal transduction.

Pathways

IQGAP1 integrates signals from different pathways including the MAPK/ERK and Wnt pathways. It modulates the MAPK pathway by interacting with Raf and MEK kinases impacting cell proliferation and differentiation. In the Wnt signaling pathway IQGAP1 binds to APC and beta-catenin regulating gene transcription related to cell growth and division. These interactions highlight its role in coordinating cellular responses to various external signals.

Associated diseases and disorders

IQGAP1 has associations with cancer and neurological disorders. In cancer alterations in IQGAP1 expression or function can lead to abnormal cell growth and metastasis due to disrupted signaling pathways like MAPK. Mutations and dysregulation of proteins such as Ras and beta-catenin commonly interact with IQGAP1 in oncogenic contexts. In neurological disorders its interaction with the actin cytoskeleton and signaling proteins can affect synapse formation and function with links to conditions such as schizophrenia and Alzheimer's disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490), expandable thumbnail

    Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: IQGAP knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: HEK293 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133490 observed at 190 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, observed at 52 kDa.
    ab133490 was shown to specifically react with IQGAP1 when IQGAP1 knockout samples were used. Wild-type and IQGAP1 knockout samples were subjected to SDS-PAGE. ab133490 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (loading control to alpha tubulin) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)

    Predicted band size: 189 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] (ab133490), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] (ab133490)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling IQGAP1 with Purified ab133490 at 1/500 dilution (5 μg/ml). Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] (ab133490), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] (ab133490)

    Immunohistochemical analysis of IQGAP1 in paraffin embedded Human kidney tissue, using ab133490 at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] (ab133490), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] (ab133490)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IQGAP1 with unpurified ab133490 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490), expandable thumbnail

    Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)

    All lanes: Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: Human placenta lysate at 10 µg

    Lane 4: Mouse thymus lysate at 10 µg

    Lane 5: F9 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 189 kDa

    Observed band size: 195 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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