Rabbit Monoclonal IQGAP1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390), expandable thumbnail](https://content.abcam.com/products/images/iqgap1-antibody-epr5220-low-endotoxin-azide-free-ab222390--immunocytochemistry-immunofluorescence-img119362.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390), expandable thumbnail](https://content.abcam.com/products/images/iqgap1-antibody-epr5220-low-endotoxin-azide-free-ab222390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90334.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390)")
![Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390), expandable thumbnail](https://content.abcam.com/products/images/iqgap1-antibody-epr5220-low-endotoxin-azide-free-ab222390--flow-cytometry-intracellular-img37265.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390)")
Publications
Filter by
- PloS one 10:e01337702015The Overexpression of IQGAP1 and β-Catenin Is Associated with Tumor Progression in Hepatocellular Carcinoma In Vitro and In Vivo.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Expected | Not recommended | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
8 products for Alternative Version
Target data
Function
Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth (PubMed:15695813). May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed:20883816).
Alternative names
KIAA0051, IQGAP1, Ras GTPase-activating-like protein IQGAP1, p195
Recommended products
Rabbit Monoclonal IQGAP1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5220
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab222390 is the carrier-free version of Anti-IQGAP1 antibody [EPR5220] ab133490.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IQGAP1 also known as IQ motif containing GTPase activating protein 1 acts as a scaffold protein that interacts with several signaling molecules. It weighs approximately 189 kDa. This protein is expressed in various tissues but shows high expression in the brain liver and kidney. IQGAP1 contains multiple domains including four IQ motifs that bind to calmodulin and a Rho GTPase-activating protein-related domain that mediates interactions with GTPases.
- Biological function summary
IQGAP1 plays a critical role in cytoskeletal organization cell adhesion and intracellular signaling. It participates in the formation of multiprotein complexes facilitating communication and structural integrity within the cell. IQGAP1 stabilizes the actin cytoskeleton and affects cell motility and polarity. It interacts with proteins such as E-cadherin and beta-catenin affecting cell-cell adhesion and signal transduction.
- Pathways
IQGAP1 integrates signals from different pathways including the MAPK/ERK and Wnt pathways. It modulates the MAPK pathway by interacting with Raf and MEK kinases impacting cell proliferation and differentiation. In the Wnt signaling pathway IQGAP1 binds to APC and beta-catenin regulating gene transcription related to cell growth and division. These interactions highlight its role in coordinating cellular responses to various external signals.
- Associated diseases and disorders
IQGAP1 has associations with cancer and neurological disorders. In cancer alterations in IQGAP1 expression or function can lead to abnormal cell growth and metastasis due to disrupted signaling pathways like MAPK. Mutations and dysregulation of proteins such as Ras and beta-catenin commonly interact with IQGAP1 in oncogenic contexts. In neurological disorders its interaction with the actin cytoskeleton and signaling proteins can affect synapse formation and function with links to conditions such as schizophrenia and Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390), expandable thumbnail](https://content.abcam.com/products/images/iqgap1-antibody-epr5220-low-endotoxin-azide-free-ab222390--immunocytochemistry-immunofluorescence-img119362.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390)")
Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling IQGAP1 with Purified Anti-IQGAP1 antibody [EPR5220] ab133490 at 1/500 dilution (5 µg/ml). Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IQGAP1 antibody [EPR5220] ab133490).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390), expandable thumbnail](https://content.abcam.com/products/images/iqgap1-antibody-epr5220-low-endotoxin-azide-free-ab222390--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90334.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390)
Immunohistochemical analysis of IQGAP1 in paraffin embedded Human kidney tissue, using Anti-IQGAP1 antibody [EPR5220] ab133490 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IQGAP1 antibody [EPR5220] ab133490).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390), expandable thumbnail](https://content.abcam.com/products/images/iqgap1-antibody-epr5220-low-endotoxin-azide-free-ab222390--flow-cytometry-intracellular-img37265.jpg "Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390)")
Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (ab222390)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IQGAP1 with unpurified Anti-IQGAP1 antibody [EPR5220] ab133490 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IQGAP1 antibody [EPR5220] ab133490).
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