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AB233030

Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal IQGAP2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Ras GTPase-activating-like protein IQGAP2, IQGAP2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling IQGAP2 with purified ab187153 at 1 : 100 dilution (1.04 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

Negative control : PBS instead of the primary antibody.

Hematoxylin was used as a counterstain

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187153).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma labeling IQGAP2 with ab187153 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187153).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)

Immunohistochemical analysis of paraffin-embedded Mouse testis labeling IQGAP2 with unpurified ab187153 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187153).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13382-9

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab233030 is the carrier-free version of ab187153.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IQGAP2 also known as Ras GTPase-activating-like protein IQGAP2 is a protein involved in intracellular signaling and the dynamics of the cytoskeleton. The molecular mass of IQGAP2 is approximately 180 kDa. It is expressed in various tissues with significant expression levels observed in the liver and stomach. IQGAP2 functions as a scaffold protein interacting with numerous cellular components to modulate their activities.
Biological function summary

IQGAP2 influences several cellular processes including cell adhesion migration and signaling pathways. It interacts with actin filaments and participates in the Rac1 signaling complex. By binding with calmodulin and other partners IQGAP2 regulates the cytoskeletal architecture contributing to cellular morphology and movement. Through its involvement in cellular interactions IQGAP2 plays roles in both normal physiological functions and pathological conditions.

Pathways

IQGAP2 participates in the MAPK and Wnt signaling pathways. In these pathways it interacts with proteins like beta-catenin and ERK influencing signal transduction that affects cell growth and differentiation. IQGAP2's role in these pathways demonstrates its significance in regulating cellular responses to environmental cues and its impact on developmental processes and tissue homeostasis.

IQGAP2's dysfunction associates with cancer particularly hepatocellular carcinoma and gastric cancer. Its interaction with beta-catenin and E-cadherin links it to the pathways that influence tumorigenesis and cancer cell adhesion. Variations in IQGAP2 expression or activity can disrupt cellular architecture and signaling contributing to disease progression and metastasis in these malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
See full target information Ras GTPase-activating-like protein IQGAP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1213-1227 PubMed40259083

2025

Multi-omic and spatial analysis of mouse kidneys highlights sex-specific differences in gene regulation across the lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Chen,Ruiyang Liu,Chia-Kuei Mo,Michael C Wendl,Andrew Houston,Preet Lal,Yanyan Zhao,Wagma Caravan,Andrew T Shinkle,Atieh Abedin-Do,Nataly Naser Al Deen,Kazuhito Sato,Xiang Li,André Luiz N Targino da Costa,Yize Li,Alla Karpova,John M Herndon,Maxim N Artyomov,Joshua B Rubin,Sanjay Jain,Xue Li,Sheila A Stewart,Li Ding,Feng Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com