Rabbit Recombinant Monoclonal IQGAP2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030), expandable thumbnail](https://content.abcam.com/products/images/iqgap2-antibody-epr13382-9-bsa-and-azide-free-ab233030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29932.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030), expandable thumbnail](https://content.abcam.com/products/images/iqgap2-antibody-epr13382-9-bsa-and-azide-free-ab233030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111182.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030), expandable thumbnail](https://content.abcam.com/products/images/iqgap2-antibody-epr13382-9-bsa-and-azide-free-ab233030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112975.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (AB233030)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Predicted |
| Rat | Expected | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
Alternative names
Ras GTPase-activating-like protein IQGAP2, IQGAP2
Recommended products
Rabbit Recombinant Monoclonal IQGAP2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13382-9
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab233030 is the carrier-free version of Anti-IQGAP2 antibody [EPR13382-9] ab187153.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IQGAP2 also known as Ras GTPase-activating-like protein IQGAP2 is a protein involved in intracellular signaling and the dynamics of the cytoskeleton. The molecular mass of IQGAP2 is approximately 180 kDa. It is expressed in various tissues with significant expression levels observed in the liver and stomach. IQGAP2 functions as a scaffold protein interacting with numerous cellular components to modulate their activities.
- Biological function summary
IQGAP2 influences several cellular processes including cell adhesion migration and signaling pathways. It interacts with actin filaments and participates in the Rac1 signaling complex. By binding with calmodulin and other partners IQGAP2 regulates the cytoskeletal architecture contributing to cellular morphology and movement. Through its involvement in cellular interactions IQGAP2 plays roles in both normal physiological functions and pathological conditions.
- Pathways
IQGAP2 participates in the MAPK and Wnt signaling pathways. In these pathways it interacts with proteins like beta-catenin and ERK influencing signal transduction that affects cell growth and differentiation. IQGAP2's role in these pathways demonstrates its significance in regulating cellular responses to environmental cues and its impact on developmental processes and tissue homeostasis.
- Associated diseases and disorders
IQGAP2's dysfunction associates with cancer particularly hepatocellular carcinoma and gastric cancer. Its interaction with beta-catenin and E-cadherin links it to the pathways that influence tumorigenesis and cancer cell adhesion. Variations in IQGAP2 expression or activity can disrupt cellular architecture and signaling contributing to disease progression and metastasis in these malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030), expandable thumbnail](https://content.abcam.com/products/images/iqgap2-antibody-epr13382-9-bsa-and-azide-free-ab233030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29932.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling IQGAP2 with purified Anti-IQGAP2 antibody [EPR13382-9] ab187153 at 1:100 dilution (1.04 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IQGAP2 antibody [EPR13382-9] ab187153).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030), expandable thumbnail](https://content.abcam.com/products/images/iqgap2-antibody-epr13382-9-bsa-and-azide-free-ab233030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111182.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)
Immunohistochemical analysis of paraffin-embedded Mouse testis labeling IQGAP2 with unpurified Anti-IQGAP2 antibody [EPR13382-9] ab187153 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IQGAP2 antibody [EPR13382-9] ab187153).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030), expandable thumbnail](https://content.abcam.com/products/images/iqgap2-antibody-epr13382-9-bsa-and-azide-free-ab233030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112975.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)
Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma labeling IQGAP2 with Anti-IQGAP2 antibody [EPR13382-9] ab187153 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IQGAP2 antibody [EPR13382-9] ab187153).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com