AB88353

Anti-IQGAP3 antibody

Name: Anti-IQGAP3 antibody (ab88353) | Abcam
Brand: Abcam Limited
SKU: AB88353
Price: 535 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(2 Publications)

Mouse Polyclonal IQGAP3 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ras GTPase-activating-like protein IQGAP3.

View Alternative Names

Ras GTPase-activating-like protein IQGAP3, IQGAP3

3 Images

AbReview44135****

Western blot - Anti-IQGAP3 antibody (AB88353)

All lanes:

Western blot - Anti-IQGAP3 antibody (ab88353) at 1/500 dilution

Lanes 1 - 2:

Mouse lung whole tissue lysate at 20 µg

Lanes 3 - 4:

Mouse lung adenocarcinoma whole tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated rabbit anti-mouse IgG monoclonal at 1/5000 dilution

Predicted band size: 185 kDa

Observed band size: 180 kDa

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Exposure time: 15min

This image is courtesy of an anonymous Abreview

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Western blot - Anti-IQGAP3 antibody (AB88353)

All lanes:

Western blot - Anti-IQGAP3 antibody (ab88353) at 1 µg/mL

All lanes:

Human placenta lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG at 1/5000 dilution

Predicted band size: 185 kDa

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Western blot - Anti-IQGAP3 antibody (AB88353)

All lanes:

Western blot - Anti-IQGAP3 antibody (ab88353) at 1 µg/mL

Lane 1:

IQGAP3-transfected 293T cell lysate at 25 µg

Lane 2:

Untransfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG at 1/5000 dilution

Predicted band size: 185 kDa

Observed band size: 179 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Ras GTPase-activating-like protein IQGAP3.

Q86VI3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target IQGAP3 also known as IQ motif containing GTPase activating protein 3 is a protein with a mass of approximately 189 kDa. Mechanically IQGAP3 functions as a scaffolding protein and interacts with cytoskeletal elements and signaling molecules. It plays important roles in the regulation of cell morphology and motility. Practically this protein is expressed in various tissues including brain lung and liver with notable roles in the nervous and respiratory systems.

Biological function summary

IQGAP3 contributes to the regulation of cell adhesion migration and proliferation. This protein operates as part of a multi-protein complex where it interacts with actin filaments and signaling molecules like Cdc42 and Rac1. These interactions facilitate the remodeling of the cytoskeleton thereby influencing cellular dynamics and functions necessary for tissue development and repair.

Pathways

IQGAP3 is involved in the MAPK/ERK signaling and PI3K/AKT pathways. This protein cooperates closely with MAPK1 (ERK2) and AKT1 both of which are important for transducing signals related to cell survival and proliferation. IQGAP3’s interactions with these pathways suggest its role in regulating processes such as cell cycle progression and response to extrinsic signals.

Researchers have linked IQGAP3 to cancer and neurological disorders. IQGAP3 affects tumorigenesis especially in colorectal cancer partly due to its interaction with beta-catenin. Furthermore its expression and activity correlate with neural development alterations hinting at a connection to neurodevelopmental disorders. Given its interactions with proteins like APC and Rac1 studying IQGAP3 can offer insights into its roles in disease pathogenesis and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ras GTPase-activating-like protein IQGAP3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical jo 25:7666-7674 PubMed31605603

2019

IQ Motif Containing GTPase-Activating Protein 3 (IQGAP3) Inhibits Kaempferol-Induced Apoptosis in Breast Cancer Cells by Extracellular Signal-Regulated Kinases 1/2 (ERK1/2) Signaling Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gaowu Hu,Huajiang Liu,Ming Wang,Wei Peng

Cancer cell international 19:163 PubMed31223291

2019

Clinical value of detecting IQGAP3, B7-H4 and cyclooxygenase-2 in the diagnosis and prognostic evaluation of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Huihua Cao,Qing Wang,Zhenyan Gao,Xiang Xu,Qicheng Lu,Yugang Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IQGAP3 antibody

Western blot - Anti-IQGAP3 antibody (AB88353)

Western blot - Anti-IQGAP3 antibody (AB88353)

Western blot - Anti-IQGAP3 antibody (AB88353)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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