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AB208009

Anti-IRAK-1 antibody [EPR19667]

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(2 Publications)

Rabbit Recombinant Monoclonal IRAK-1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody [EPR19667] (AB208009)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody [EPR19667] (AB208009)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling IRAK-1 with ab208009 at 1/100 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :

-ve control 1 : ab208009 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.

-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.

Western blot - Anti-IRAK-1 antibody [EPR19667] (AB208009)
  • WB

Supplier Data

Western blot - Anti-IRAK-1 antibody [EPR19667] (AB208009)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/3/4 : 1 minute; Lane 2 : 30 seconds.

There are two splice variants for IRAK-1, IRAK-1b and IRAK-1c,respectively. The product recognizes full length IRAK-1 (80 kDa) and IRAK-1c (68 kDa) (PMID : 16690127).

All lanes:

Western blot - Anti-IRAK-1 antibody [EPR19667] (ab208009) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 76 kDa

Observed band size: 68 kDa,80 kDa

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Western blot - Anti-IRAK-1 antibody [EPR19667] (AB208009)
  • WB

Lab

Western blot - Anti-IRAK-1 antibody [EPR19667] (AB208009)

Lane 1 : Wild type HAP1 whole cell lysate (40 μg)
Lane 2 : IRAK-1 knockout HAP1 whole cell lysate (40 μg)
Lane 3 : HeLa whole cell lysate (40 μg)
Lane 4 : HEK293 whole cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab208009 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab208009 was shown to specifically react with IRAK-1 when IRAK-1 knockout samples were used. Wild-type and IRAK-1 knockout samples were subjected to SDS-PAGE. ab208009 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 200 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-IRAK-1 antibody [EPR19667] (ab208009)

Predicted band size: 76 kDa

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  • Carrier free

    Anti-IRAK-1 antibody [EPR19667] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19667

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.
Biological function summary

IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.

Pathways

IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.

IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
See full target information IRAK1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of healthcare engineering 2021:2982924 PubMed34931135

2021

MiR-301a-3p Advances IRAK1-Mediated Differentiation of Th17 Cells to Promote the Progression of Systemic Lupus Erythematosus via Targeting PELI1.

Applications

Unspecified application

Species

Unspecified reactive species

Shuaihantian Luo,Ruifang Wu,Qianwen Li,Guiying Zhang

Cell death & disease 9:1123 PubMed30409964

2018

miR-146b promotes cell proliferation and increases chemosensitivity, but attenuates cell migration and invasion via FBXL10 in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Meina Yan,Xinxin Yang,Rong Shen,Chengjiang Wu,Hui Wang,Qing Ye,Peifang Yang,Lubin Zhang,Miao Chen,Bing Wan,Qinqin Zhang,Sheng Xia,Xiaodong Lu,Genbao Shao,Xiaoming Zhou,Jun Yu,Qixiang Shao
View all publications
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