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Rabbit Recombinant Monoclonal IRAK-1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (AB302555), expandable thumbnail
  • Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (AB302555), expandable thumbnail
  • Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (AB302555), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (AB302555), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (AB302555), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer
Standard buffer
Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Alternative names

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1

Recommended products

Rabbit Recombinant Monoclonal IRAK-1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26375-90
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.

Biological function summary

IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.

Pathways

IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.

Associated diseases and disorders

IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555), expandable thumbnail

    Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555)

    This data was developed using Anti-IRAK-1 antibody [EPR26375-90] ab302554, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-IRAK-1 antibody [EPR26375-90] (Anti-IRAK-1 antibody [EPR26375-90] ab302554) at 1/500 dilution

    All lanes: Human hypothalamus tissue lysate 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

    Exposure time: 59s

  • Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555), expandable thumbnail

    Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555)

    This data was developed using Anti-IRAK-1 antibody [EPR26375-90] ab302554, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-IRAK-1 antibody [EPR26375-90] (Anti-IRAK-1 antibody [EPR26375-90] ab302554) at 1/500 dilution

    All lanes: Human tonsil tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 80 kDa

    Exposure time: 180s

  • Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555), expandable thumbnail

    Western blot - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555)

    This data was developed using Anti-IRAK-1 antibody [EPR26375-90] ab302554, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBST
    The samples were run on a Bis-Tris gel.Lysates/proteins at 20 µg per lane. Performed under reducing conditions.False colour image of Western blot: Anti-IRAK1 antibody (Anti-IRAK-1 antibody [EPR26375-90] ab302554) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.In Western blot, Anti-IRAK-1 antibody [EPR26375-90] ab302554 was shown to bind specifically to IRAK1. A band was observed at 80 kDa in wild-type HAP1 cell lysates with no signal observed at this size in IRAK1 knockout cell line. To generate this image, wild-type and IRAK1 knockout HAP1 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-IRAK-1 antibody [EPR26375-90] (Anti-IRAK-1 antibody [EPR26375-90] ab302554) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate 20 µg

    Lane 2: IRAK1 knockout HAP1 whole cell lysate 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Lanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555)

    This data was developed using Anti-IRAK-1 antibody [EPR26375-90] ab302554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded A Wild-type HAP1 (H tissue labeling IRAK-1 with Anti-IRAK-1 antibody [EPR26375-90] ab302554 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) wild-type HAP1 cell pellet, no staining on (B) IRAK1 knockout HAP1 cell pellet. The section was incubated with Anti-IRAK-1 antibody [EPR26375-90] ab302554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555)

    This data was developed using Anti-IRAK-1 antibody [EPR26375-90] ab302554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human lung cancer an tissue labeling IRAK-1 with Anti-IRAK-1 antibody [EPR26375-90] ab302554 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human lung cancer, no staining in the adjacent tissue. The section was incubated with Anti-IRAK-1 antibody [EPR26375-90] ab302554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody [EPR26375-90] (BSA and Azide free) (ab302555)

    This data was developed using Anti-IRAK-1 antibody [EPR26375-90] ab302554, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling IRAK-1 with Anti-IRAK-1 antibody [EPR26375-90] ab302554 at 1/100 (4.53 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human colon carcinoma. The section was incubated with Anti-IRAK-1 antibody [EPR26375-90] ab302554 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

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