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AB218130

Anti-IRAK-1 (phospho T209) antibody

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(13 Publications)

Rabbit Polyclonal IRAK-1 phospho T209 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Mouse Irak1 phospho T209 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Il1rak, Irak1, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK-1, Pelle-like protein kinase, mPLK

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 (phospho T209) antibody (AB218130)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 (phospho T209) antibody (AB218130)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse placenta tissue, labeling IRAK-1 using ab218130 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Irak1 phospho T209 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q62406

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.
Biological function summary

IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.

Pathways

IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.

IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3 (By similarity).
See full target information Irak1 phospho T209
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1366319 PubMed38799464

2024

MiR-146a alleviates inflammatory bowel disease in mice through systematic regulation of multiple genetic networks.

Applications

Unspecified application

Species

Unspecified reactive species

Fengting Zhu,Taotan Yang,Mengmeng Ning,Yang Liu,Wei Xia,Yan Fu,Ting Wen,Mei Zheng,Ruilong Xia,Ran Qian,Yang Li,Minxuan Sun,Jianping Liu,Li Tian,Qian Zhou,Xin Yu,Changgeng Peng

Blood cancer journal 13:125 PubMed37591861

2023

The oncogenic human B-cell lymphoma MYD88 L265P mutation genocopies activation by phosphorylation at the Toll/interleukin-1 receptor (TIR) domain.

Applications

Unspecified application

Species

Unspecified reactive species

Marthe Minderman,Hildo Lantermans,Carmen van der Zwaan,Arie J Hoogendijk,Maartje van den Biggelaar,Marie José Kersten,Marcel Spaargaren,Steven T Pals

Signal transduction and targeted therapy 8:236 PubMed37332010

2023

Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Yuan Zhang,Qiu-Ping Xu,Li-Na Shi,Shih-Wen Li,Wei-Hong Wang,Qing-Qing Wang,Liao-Xun Lu,Hui Xiao,Jun-Hong Wang,Feng-Ying Li,Yin-Ming Liang,Si-Tang Gong,Hao-Ran Peng,Zheng Zhang,Hong Tang

Evidence-based complementary and alternative medicine : eCAM 2022:5161890 PubMed36467552

2022

Paeoniflorin Inhibits LPS-Induced Activation of Splenic CD4 T Lymphocytes and Relieves Pathological Symptoms in MRL/lpr Mice by Suppressing IRAK1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Ji,Shenglong Wang,Shan Wu,Jie Bao,Guanqun Xie,Yan Zhang,Liping Xu,Na Lin,Jian Wang,Yongsheng Fan,Danqing Fu,Qiaoding Dai

Frontiers in pharmacology 13:952775 PubMed36210830

2022

Triptolide in the treatment of systemic lupus erythematosus - regulatory effects on miR-146a in B cell TLR7 signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,FengQi Zhang,YiNi Gao,MeiJiao Wang,Yan Gao,HaiChang Li,Jing Sun,ChengPing Wen,ZhiJun Xie

British journal of pharmacology 179:5089-5108 PubMed35760458

2022

6-Shogaol (enexasogoal) treatment improves experimental knee osteoarthritis exerting a pleiotropic effect over immune innate signalling responses in chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Gratal,Aránzazu Mediero,Ana Lamuedra,Alejandra Matamoros-Recio,Carmen Herencia,Gabriel Herrero-Beaumont,Sonsoles Martín-Santamaría,Raquel Largo

Bioengineered 13:5005-5020 PubMed35164650

2022

Somatostatin attenuates intestinal epithelial barrier injury during acute intestinal ischemia-reperfusion through Tollip/Myeloiddifferentiationfactor 88/Nuclear factor kappa-B signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Tian,Ruo Shu,Yi Lei,Yu Xu,Xinfeng Zhang,Huayou Luo

British journal of pharmacology 178:4048-4068 PubMed34076894

2021

Neutrophil elastase promotes neointimal hyperplasia by targeting toll-like receptor 4 (TLR4)-NF-κB signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Yang,Qishan Chen,Li Mei,Guanmei Wen,Weiwei An,Xinmiao Zhou,Kaiyuan Niu,Chenxin Liu,Meixia Ren,Kun Sun,Qingzhong Xiao,Li Zhang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21519 PubMed34137477

2021

Suppression of cigarette smoke induced MMP1 expression by selective serotonin re-uptake inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Gerber Adam,Takayuki Shiomi,Goldklang Monica,Sonett Jarrod,Anguiano Vincent,Mercer Becky,Zelonina Tina,D'Armiento Jeanine

Frontiers in endocrinology 11:522340 PubMed33329376

2020

Overexpression of microRNA-216a-3p Accelerates the Inflammatory Response in Cardiomyocytes in Type 2 Diabetes Mellitus by Targeting IFN-α2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Liu,Yusong Zhang,Hongwei Liang,Yanchao Xu
View all publications
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