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AB6148

Anti-IRAK2 antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal IRAK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IRAK2 aa 500-600.

View Alternative Names

Interleukin-1 receptor-associated kinase-like 2, IRAK-2, IRAK2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB6148)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB6148)

ab6148 at 2μg/ml staining IRAK2 in Hela cells by ICC/IF

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB6148)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB6148)

Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB6148)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB6148)

Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.

Western blot - Anti-IRAK2 antibody (AB6148)
  • WB

Unknown

Western blot - Anti-IRAK2 antibody (AB6148)

All lanes:

Western blot - Anti-IRAK2 antibody (ab6148) at 2 µg/mL

Lane 1:

HeLa whole cell lysate

Lane 2:

K562 whole cell lysate

Predicted band size: 69 kDa

Observed band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human IRAK2 aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

O43187

Specificity

Anti-IRAK2 has no cross response to IRAK.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified IgG was prepared by affinity chromatography.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK2 also known as Interleukin-1 Receptor-Associated Kinase 2 plays a central role in the immune response. This protein has a molecular mass of approximately 68 kDa. It is broadly expressed in various tissues particularly in those related to the immune system including the spleen thymus and peripheral blood leukocytes. IRAK2 is a part of the IRAK family which includes other kinases like IRAK1 IRAK3 and IRAK4 sharing structural similarities.
Biological function summary

The IRAK2 protein is critical for transducing signals from interleukin-1 receptors (IL-1R) and Toll-like receptors (TLR) as part of the innate immune response. It does not work alone but as part of the Myddosome complex. This complex formation is essential for signal propagation leading to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinases (MAPKs). Activation of these pathways initiates inflammatory and immune processes enabling the body to respond to pathogens.

Pathways

IRAK2 integrates into the Toll-like receptor signaling pathway acting as a link between upstream receptor activation and downstream transcription factors. It has strong interactions with other key immune components like MyD88 and TRAF6 within these pathways. Through these links IRAK2 contributes to the orchestration of a coordinated immune defense facilitating the body's capacity to manage microbial infections while maintaining regulatory checks to prevent excessive inflammation.

Defects or dysregulation in IRAK2 function can lead to immune-related diseases. For instance aberrant IRAK2 activity associates with rheumatoid arthritis where it drives inflammatory processes contributing to joint damage. Moreover its involvement in the immune signaling network makes it relevant in sepsis a condition resulting from an overwhelming immune response to infection. In these disorders IRAK2 works in conjunction with other proteins such as IRAK1 and TRAF6 influencing disease progression and symptom manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
See full target information IRAK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of chronic obstructive pulmonary disease 20:1543-1553 PubMed40417409

2025

A Peptide Encoded by lncRNA HOXB-AS3 Promotes Cigarette Smoke-Induced Inflammation in Bronchial Epithelial Cells via EZH2-Mediated H3K27me3 Modification.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Lin,Xiaoman Zhou,Yixiu Yang,Pingdong Xie,Quanni Li,Chanyi He,Qi Lin,Xingwei Wei,Yipeng Ding
View all publications
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Product promise

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