AB62419

Anti-IRAK2 antibody

Name: Anti-IRAK2 antibody (ab62419) | Abcam
Brand: Abcam Limited
SKU: AB62419
Price: 585 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(16 Publications)

Rabbit Polyclonal IRAK2 antibody. Suitable for ICC, IP, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Mouse Irak2.

View Alternative Names

Interleukin-1 receptor-associated kinase-like 2, IRAK-2, IRAK2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB62419)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB62419)

Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.

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Immunocytochemistry - Anti-IRAK2 antibody (AB62419)

Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml.

AbReview38052****

Western blot - Anti-IRAK2 antibody (AB62419)

Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1μg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4°C. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.

All lanes:

Western blot - Anti-IRAK2 antibody (ab62419)

Predicted band size: 69 kDa

Observed band size: 70 kDa

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Exposure time: 15s

This image is courtesy of an anonymous abreview.

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Western blot - Anti-IRAK2 antibody (AB62419)

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Western blot - Anti-IRAK2 antibody (ab62419) at 0.5 µg/mL

Lane 2:

Western blot - Anti-IRAK2 antibody (ab62419) at 1 µg/mL

Lane 3:

Western blot - Anti-IRAK2 antibody (ab62419) at 2 µg/mL

Lane 1:

RAW264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 15 µg

Lanes 2 - 3:

RAW264.7 whole cell lysate at 15 µg

Predicted band size: 69 kDa

Observed band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC, IP, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Irak2. The exact immunogen used to generate this antibody is proprietary information.

Q8CFA1

Specificity

This antibody reacts with IRAK2, it does not react with IRAK

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "(PMID 19224918)", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "(PMID 19224918)", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK2 also known as Interleukin-1 Receptor-Associated Kinase 2 plays a central role in the immune response. This protein has a molecular mass of approximately 68 kDa. It is broadly expressed in various tissues particularly in those related to the immune system including the spleen thymus and peripheral blood leukocytes. IRAK2 is a part of the IRAK family which includes other kinases like IRAK1 IRAK3 and IRAK4 sharing structural similarities.

Biological function summary

The IRAK2 protein is critical for transducing signals from interleukin-1 receptors (IL-1R) and Toll-like receptors (TLR) as part of the innate immune response. It does not work alone but as part of the Myddosome complex. This complex formation is essential for signal propagation leading to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinases (MAPKs). Activation of these pathways initiates inflammatory and immune processes enabling the body to respond to pathogens.

Pathways

IRAK2 integrates into the Toll-like receptor signaling pathway acting as a link between upstream receptor activation and downstream transcription factors. It has strong interactions with other key immune components like MyD88 and TRAF6 within these pathways. Through these links IRAK2 contributes to the orchestration of a coordinated immune defense facilitating the body's capacity to manage microbial infections while maintaining regulatory checks to prevent excessive inflammation.

Defects or dysregulation in IRAK2 function can lead to immune-related diseases. For instance aberrant IRAK2 activity associates with rheumatoid arthritis where it drives inflammatory processes contributing to joint damage. Moreover its involvement in the immune signaling network makes it relevant in sepsis a condition resulting from an overwhelming immune response to infection. In these disorders IRAK2 works in conjunction with other proteins such as IRAK1 and TRAF6 influencing disease progression and symptom manifestation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.

See full target information IRAK2

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 31:753-767 PubMed38605168

2024

OTUD5 promotes the inflammatory immune response by enhancing MyD88 oligomerization and Myddosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxing Liu,Jiahua Yuan,Yuling Zhang,Fei Qin,Xuemei Bai,Wanwei Sun,Tian Chen,Feng Liu,Yi Zheng,Xiaopeng Qi,Wei Zhao,Bingyu Liu,Chengjiang Gao

Cell reports 43:113795 PubMed38367238

2024

The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection.

Applications

Unspecified application

Species

Unspecified reactive species

Milton Pereira,Theresa Ramalho,Warrison A Andrade,Danielle F Durso,Maria C Souza,Katherine A Fitzgerald,Douglas T Golenbock,Neal Silverman,Ricardo T Gazzinelli

The Biochemical journal 479:2419-2431 PubMed36408944

2022

Identification of ester-linked ubiquitylation sites during TLR7 signalling increases the number of inter-ubiquitin linkages from 8 to 12.

Applications

Unspecified application

Species

Unspecified reactive species

Elisha H McCrory,Vyacheslav Akimov,Philip Cohen,Blagoy Blagoev

Cell reports 40:111225 PubMed35977521

2022

The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Milton Pereira,Danielle F Durso,Clare E Bryant,Evelyn A Kurt-Jones,Neal Silverman,Douglas T Golenbock,Ricardo T Gazzinelli

Nature communications 13:2748 PubMed35585086

2022

IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Liu,Hao Zhou,Han Wang,Quanri Zhang,Renliang Zhang,Belinda Willard,Caini Liu,Zizhen Kang,Xiao Li,Xiaoxia Li

Nature immunology 21:1219-1231 PubMed32778760

2020

IL-1 induces mitochondrial translocation of IRAK2 to suppress oxidative metabolism in adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhou,Han Wang,Minjia Yu,Rebecca C Schugar,Wen Qian,Fangqiang Tang,Weiwei Liu,Hui Yang,Ruth E McDowell,Junjie Zhao,Ji Gao,Ashok Dongre,Julie A Carman,Mei Yin,Judith A Drazba,Robert Dent,Christopher Hine,Yeong-Renn Chen,Jonathan D Smith,Paul L Fox,J Mark Brown,Xiaoxia Li

Biology open 9: PubMed32414769

2020

lncRNA NEAT1 mediates sepsis progression by regulating Irak2 via sponging miR-370-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xiao,Chuihua Sun,Ying Xiao,Yunbao Li

eLife 6: PubMed28990926

2017

IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhou,Katarzyna Bulek,Xiao Li,Tomasz Herjan,Minjia Yu,Wen Qian,Han Wang,Gao Zhou,Xing Chen,Hui Yang,Lingzi Hong,Junjie Zhao,Luke Qin,Koichi Fukuda,Annette Flotho,Ji Gao,Ashok Dongre,Julie A Carman,Zizhen Kang,Bing Su,Timothy S Kern,Jonathan D Smith,Thomas A Hamilton,Frauke Melchior,Paul L Fox,Xiaoxia Li

Molecular medicine reports 16:7205-7212 PubMed28944871

2017

Shear stress induces human aortic endothelial cell apoptosis via interleukin‑1 receptor‑associated kinase 2‑induced endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Longfei Pan,Zhou Hong,Lei Yu,Yanxia Gao,Rui Zhang,Hui Feng,Lijuan Su,Gang Wang

Scientific reports 6:31159 PubMed27498693

2016

Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria A McGuire,Tamara Ruiz-Zorrilla Diez,Christoph H Emmerich,Sam Strickson,Maria Stella Ritorto,Ruhcha V Sutavani,Anne Weiβ,Kirsty F Houslay,Axel Knebel,Paul J Meakin,Iain R Phair,Michael L J Ashford,Matthias Trost,J Simon C Arthur

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IRAK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (AB62419)

Immunocytochemistry - Anti-IRAK2 antibody (AB62419)

Western blot - Anti-IRAK2 antibody (AB62419)

Western blot - Anti-IRAK2 antibody (AB62419)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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