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AB251120

Anti-IRAK2 antibody [EPR16530] - BSA and Azide free

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Rabbit Recombinant Monoclonal IRAK2 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Interleukin-1 receptor-associated kinase-like 2, IRAK-2, IRAK2

4 Images
Flow Cytometry (Intracellular) - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)

This data was developed using ab192249, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MOLT4 cells labeling IRAK2 with ab192249 at 1/220 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)
  • IP

Supplier Data

Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)

This data was developed using ab192249, the same antibody clone in a different buffer formulation.

Western blot analysis of IRAK2 in MOLT4 cell lysates immunoprecipitated using ab192249 at 1/60 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

Blocking/Dilution buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-IRAK2 antibody [EPR16530] (<a href='/en-us/products/primary-antibodies/irak2-antibody-epr16530-ab192249'>ab192249</a>)

Predicted band size: 69 kDa

false

Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)
  • WB

Supplier Data

Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)

This data was developed using ab192249, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IRAK2 antibody [EPR16530] (<a href='/en-us/products/primary-antibodies/irak2-antibody-epr16530-ab192249'>ab192249</a>) at 1/1000 dilution

Lane 1:

THP1 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 69 kDa

false

Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)
  • WB

Supplier Data

Western blot - Anti-IRAK2 antibody [EPR16530] - BSA and Azide free (AB251120)

This data was developed using ab192249, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IRAK2 antibody [EPR16530] (<a href='/en-us/products/primary-antibodies/irak2-antibody-epr16530-ab192249'>ab192249</a>) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16530

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB220136

Human IRAK-1 Matched Antibody Pair Kit

Recombinant

ELISA - Human IRAK-1 Matched Antibody Pair Kit (AB220136)

  • 0
  • 0
Primary Antibodies

AB208009

Anti-IRAK-1 antibody [EPR19667]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody [EPR19667] (AB208009)

  • 0
  • 0
Primary Antibodies

AB32394

Anti-IRAKM antibody [Y278]

RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab251120 is the carrier-free version of ab192249.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK2 also known as Interleukin-1 Receptor-Associated Kinase 2 plays a central role in the immune response. This protein has a molecular mass of approximately 68 kDa. It is broadly expressed in various tissues particularly in those related to the immune system including the spleen thymus and peripheral blood leukocytes. IRAK2 is a part of the IRAK family which includes other kinases like IRAK1 IRAK3 and IRAK4 sharing structural similarities.
Biological function summary

The IRAK2 protein is critical for transducing signals from interleukin-1 receptors (IL-1R) and Toll-like receptors (TLR) as part of the innate immune response. It does not work alone but as part of the Myddosome complex. This complex formation is essential for signal propagation leading to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinases (MAPKs). Activation of these pathways initiates inflammatory and immune processes enabling the body to respond to pathogens.

Pathways

IRAK2 integrates into the Toll-like receptor signaling pathway acting as a link between upstream receptor activation and downstream transcription factors. It has strong interactions with other key immune components like MyD88 and TRAF6 within these pathways. Through these links IRAK2 contributes to the orchestration of a coordinated immune defense facilitating the body's capacity to manage microbial infections while maintaining regulatory checks to prevent excessive inflammation.

Defects or dysregulation in IRAK2 function can lead to immune-related diseases. For instance aberrant IRAK2 activity associates with rheumatoid arthritis where it drives inflammatory processes contributing to joint damage. Moreover its involvement in the immune signaling network makes it relevant in sepsis a condition resulting from an overwhelming immune response to infection. In these disorders IRAK2 works in conjunction with other proteins such as IRAK1 and TRAF6 influencing disease progression and symptom manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
See full target information IRAK2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com