AB5985

Anti-IRAK4 antibody

Name: Anti-IRAK4 antibody (ab5985) | Abcam
Brand: Abcam Limited
SKU: AB5985
Rating: 5 (2 reviews)

(2 Reviews)

(4 Publications)

Rabbit Polyclonal IRAK4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK4.

View Alternative Names

Interleukin-1 receptor-associated kinase 4, IRAK-4, Renal carcinoma antigen NY-REN-64, IRAK4

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

ICC/IF

AbReview17009****

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.

Left image : DAPI staining nuclei (blue)
Middle image : IRAK4 (red)
Right image : Overlay

This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview

Supplier Data

Western blot - Anti-IRAK4 antibody (AB5985)

Primary incbation : 1 hour at room temperature in 5% NFDM/TBST.

Lane 1:

Western blot - Anti-IRAK4 antibody (ab5985) at 1 µg/mL

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Western blot - Anti-IRAK4 antibody (ab5985) at 2 µg/mL

Lane 3:

Western blot - Anti-IRAK4 antibody (ab5985) at 4 µg/mL

All lanes:

HeLa cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-IRAK4 antibody (AB5985)

All lanes:

Western blot - Anti-IRAK4 antibody (ab5985) at 1 µg/mL

Lane 1:

Daudi cell lysate at 15 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

HepG2 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human IRAK4. The exact immunogen used to generate this antibody is proprietary information.

Q9NWZ3

Specificity

This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

The product can be stored for up to 12 months

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.

Biological function summary

IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.

Pathways

IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.

Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed : 17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

See full target information IRAK4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36040792

2022

Activation of targetable inflammatory immune signaling is seen in myelodysplastic syndromes with SF3B1 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Gaurav S Choudhary,Andrea Pellagatti,Bogos Agianian,Molly A Smith,Tushar D Bhagat,Shanisha Gordon-Mitchell,Srabani Sahu,Sanjay Pandey,Nishi Shah,Srinivas Aluri,Ritesh Aggarwal,Sarah Aminov,Leya Schwartz,Violetta Steeples,Robert N Booher,Murali Ramachandra,Maria Samson,Milagros Carbajal,Kith Pradhan,Teresa V Bowman,Manoj M Pillai,Britta Will,Amittha Wickrema,Aditi Shastri,Robert K Bradley,Robert E Martell,Ulrich G Steidl,Evripidis Gavathiotis,Jacqueline Boultwood,Daniel T Starczynowski,Amit Verma

Mediators of inflammation 2021:5318369 PubMed33505213

2021

lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanchuan Shi,Yuqian Zhao,Qi Li,Jianguo Li

Nature cell biology 21:640-650 PubMed31011167

2019

U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Molly A Smith,Gaurav S Choudhary,Andrea Pellagatti,Kwangmin Choi,Lyndsey C Bolanos,Tushar D Bhagat,Shanisha Gordon-Mitchell,Dagny Von Ahrens,Kith Pradhan,Violetta Steeples,Sanghyun Kim,Ulrich Steidl,Matthew Walter,Iain D C Fraser,Aishwarya Kulkarni,Nathan Salomonis,Kakajan Komurov,Jacqueline Boultwood,Amit Verma,Daniel T Starczynowski

Journal of immunology (Baltimore, Md. : 1950) 179:8491-9 PubMed18056396

2007

Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis.

Applications

Species

Sheep

Suhas G Kallapur,Alan H Jobe,Molly K Ball,Ilias Nitsos,Timothy J M Moss,Noah H Hillman,John P Newnham,Boris W Kramer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRAK4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (AB5985)

Western blot - Anti-IRAK4 antibody (AB5985)

Western blot - Anti-IRAK4 antibody (AB5985)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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