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AB216513

Anti-IRAK4 (phospho T345) antibody

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(4 Publications)

Rabbit Polyclonal IRAK4 phospho T345 antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK4 pT345 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Interleukin-1 receptor-associated kinase 4, IRAK-4, Renal carcinoma antigen NY-REN-64, IRAK4

1 Images
Western blot - Anti-IRAK4 (phospho T345) antibody (AB216513)
  • WB

Lab

Western blot - Anti-IRAK4 (phospho T345) antibody (AB216513)

All lanes:

Western blot - Anti-IRAK4 (phospho T345) antibody (ab216513) at 1/500 dilution

All lanes:

NIH/3T3 lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human IRAK4 pT345 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NWZ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.
Biological function summary

IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.

Pathways

IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.

Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed : 17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
See full target information IRAK4 pT345
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 31:746-755 PubMed39704655

2024

Smoking-Associated Carcinogen-Induced Inflammation Promotes Lung Carcinogenesis via IRAK4 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ritesh K Aggarwal,Simone Sidoli,Jingli Wang,Srabani Sahu,Rahul Sanawar,Varun Gupta,Srinivas Aluri,Vineeth Sukrithan,Charan T R Vegivinti,Phaedon D Zavras,Divij Verma,Shanisha Gordon-Mitchell,Beamon Agarwal,Tanya Verma,Daniel T Starczynowski,Ulrich G Steidl,Aditi Shastri,Balazs Halmos,Lindsay M LaFave,Haiying Cheng,Amit Verma,Yiyu Zou

Science advances 10:eadl0030 PubMed38924398

2024

KIBRA anchoring the action of PKMζ maintains the persistence of memory.

Applications

Unspecified application

Species

Unspecified reactive species

Panayiotis Tsokas,Changchi Hsieh,Rafael E Flores-Obando,Matteo Bernabo,Andrew Tcherepanov,A Iván Hernández,Christian Thomas,Peter J Bergold,James E Cottrell,Joachim Kremerskothen,Harel Z Shouval,Karim Nader,André A Fenton,Todd C Sacktor

Molecular systems biology 18:e10855 PubMed35959629

2022

Drug-microenvironment perturbations reveal resistance mechanisms and prognostic subgroups in CLL.

Applications

Unspecified application

Species

Unspecified reactive species

Peter-Martin Bruch,Holly Ar Giles,Carolin Kolb,Sophie A Herbst,Tina Becirovic,Tobias Roider,Junyan Lu,Sebastian Scheinost,Lena Wagner,Jennifer Huellein,Ivan Berest,Mark Kriegsmann,Katharina Kriegsmann,Christiane Zgorzelski,Peter Dreger,Judith B Zaugg,Carsten Müller-Tidow,Thorsten Zenz,Wolfgang Huber,Sascha Dietrich

Mediators of inflammation 2021:5318369 PubMed33505213

2021

lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanchuan Shi,Yuqian Zhao,Qi Li,Jianguo Li
View all publications
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Product promise

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