Rabbit Polyclonal IRAKM antibody. Suitable for ICC, WB, IHC-P and reacts with Rat, Mouse samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK3 aa 550 to C-terminus.
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- Translational andrology and urology 11:268-2762022Loss of NLRP3 increases bacterial cystitis via IRAKM.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Sun,Lei Xia,Yubing PengPubMed 35280657
- Bioengineered 12:10147-101592021polysaccharides (PSP) improve the palmitic acid (PA)-induced inhibition of survival, inflammation, and glucose uptake in skeletal muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Luo Cai,Xiao-Ping Li,Yi-Lin Zhu,Gang-Qiang Yi,Wei Wang,Xin-Yu Chen,Gui-Ming Deng,Lei Yang,Hu-Zhi Cai,Qiao-Zhen Tong,Li Zhou,Mengying Tian,Xin-Hua Xia,Ping-An LiuPubMed 34872451
- Molecular biology reports 49:121-1302021MiR-539-5p inhibits the inflammatory injury in septic H9c2 cells by regulating IRAK3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochen Hu,Hongjun MiaoPubMed 34757596
- Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 18:1891-19042021α-Acetylcholine Receptor Signaling Reduces Neuroinflammation After Subarachnoid Hemorrhage in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAri Dienel,Remya A Veettil,Kanako Matsumura,Jude P J Savarraj,H Alex Choi,Peeyush Kumar T,Jaroslaw Aronowski,Pramod Dash,Spiros L Blackburn,Devin W McBridePubMed 33970466
- Cancer management and research 12:7043-70592020Hypermethylation of the -Activated MAPK Signaling Pathway to Promote the Development of Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinghai Wu et. al.PubMed 32848462
- Oncotarget 8:9053-90662017Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPARγ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed A Al-Qahtani,Konstantina Lyroni,Marina Aznaourova,Melpomeni Tseliou,Mashael R Al-Anazi,Mohammed N Al-Ahdal,Saad Alkahtani,George Sourvinos,Christos TsatsanisPubMed 28118607
- Journal of immunology (Baltimore, Md. : 1950) 197:1776-872016Transduction of Functionally Contrasting Signals by Two Mycobacterial PPE Proteins Downstream of TLR2 Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtul Udgata et. al.PubMed 27481848
- PloS one 10:e01298672015Induction of Tolerogenic Dendritic Cells by a PEGylated TLR7 Ligand for Treatment of Type 1 Diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoko Hayashi et. al.PubMed 26076454
- Journal of immunology (Baltimore, Md. : 1950) 189:356-642012TLR signaling prevents hyperoxia-induced lung injury by protecting the alveolar epithelium from oxidant-mediated death.Applications:WBReactive species:Unspecified reactive speciesMegan N Ballinger et. al.PubMed 22661086
- Journal of immunology (Baltimore, Md. : 1950) 186:403-102010Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M.Applications:WBReactive species:HumanHiroshi Kobayashi,Anna Nolan,Bushra Naveed,Yoshihiko Hoshino,Leopoldo N Segal,Yoko Fujita,William N Rom,Michael D WeidenPubMed 21098228
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human IRAK3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y616
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Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Mouse | Expected | Tested | Tested |
| Rat | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-1 µg/mL | Notes - |
Species Rat | Dilution info 0.5-1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2 µg/mL | Notes - |
Species Rat | Dilution info 2 µg/mL | Notes - |
Associated Products
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Target data
Function
Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed:10383454, PubMed:29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed:29686383).
Alternative names
Interleukin-1 receptor-associated kinase 3, IRAK-3, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, IRAK-M, IRAK3
Recommended products
Rabbit Polyclonal IRAKM antibody. Suitable for ICC, WB, IHC-P and reacts with Rat, Mouse samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK3 aa 550 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human IRAK3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y616
- Purification technique
- Affinity purification
- Specificity
Anti-IRAK-M has no cross response to IRAK or IRAK2.
- Concentration
- Purification notes
Purified IgG prepared by ion exchange chromatography.IRAK-M Antibody is affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IRAK-M protein also known as IRAKM or interleukin-1 receptor-associated kinase M is a member of the IRAK family and has a molecular mass of approximately 65 kDa. It plays an important role in the immune response regulation. This protein is expressed in monocytes and macrophages but it is also found in other immune cells. Unlike other IRAKs IRAK-M acts as a negative regulator helping to ensure the immune system does not overreact.
- Biological function summary
IRAK-M inhibits the signaling pathways that lead to the activation of inflammatory responses. It predominantly affects signaling initiated by toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) preventing excessive inflammatory cytokine production. IRAK-M lacks kinase activity unlike other IRAK family members so it functions as a regulatory molecule rather than participating in kinase-mediated catalysis. It does not form part of a larger complex but interacts directly with other signaling components in these pathways.
- Pathways
IRAK-M impacts TLR and IL-1 signaling pathways. These pathways are important in the innate immune system's response to pathogens and inflammation. IRAK-M interacts with proteins such as MyD88 and TNF receptor-associated factor 6 (TRAF6) reducing the downstream production of pro-inflammatory cytokines. This modulation helps to maintain immune system balance and prevent chronic inflammation.
- Associated diseases and disorders
IRAK-M is involved in conditions such as sepsis and autoimmune diseases. In sepsis IRAK-M's alteration can lead to an inadequate immune response influencing the body's ability to control infection. In autoimmune diseases abnormal IRAK-M expression may result in regulatory failures leading to persistent inflammation and tissue damage. IRAK-M's interaction with other proteins like MyD88 suggests its pivotal role in these disorders where misregulated pathways can contribute to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.Immunocytochemistry - Anti-IRAKM antibody (ab8116)
ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.
Western blot - Anti-IRAKM antibody (ab8116)
Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1 μg /ml.
All lanes: Western blot - Anti-IRAKM antibody (ab8116) at 1 µg/mL
Lane 1: Mouse spleen tissue lysates
Lane 2: Rat liver tissue lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of A20 cells staining IRAKM with ab8116 at 20 µg/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)
Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 μg/ml.
Immunocytochemistry - Anti-IRAKM antibody (ab8116)
Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)
ab8116 at 2μg/ml staining IRAKM in rat liver cells by IHC
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