AB8116

Anti-IRAKM antibody

Name: Anti-IRAKM antibody (ab8116) | Abcam
Brand: Abcam Limited
SKU: AB8116
Price: 670 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(16 Publications)

Rabbit Polyclonal IRAKM antibody. Suitable for ICC, WB, IHC-P and reacts with Rat, Mouse samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human IRAK3 aa 550 to C-terminus.

View Alternative Names

Interleukin-1 receptor-associated kinase 3, IRAK-3, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, IRAK-M, IRAK3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (AB8116)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (AB8116)

Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 μg/ml.

Supplier Data

Immunocytochemistry - Anti-IRAKM antibody (AB8116)

Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (AB8116)

ab8116 at 2μg/ml staining IRAKM in rat liver cells by IHC

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (AB8116)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of A20 cells staining IRAKM with ab8116 at 20 µg/ml

PubMed

Immunocytochemistry - Anti-IRAKM antibody (AB8116)

ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
The cells were permeablized with a 1 : 1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.

Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.

Unknown

Western blot - Anti-IRAKM antibody (AB8116)

Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1 μg /ml.

All lanes:

Western blot - Anti-IRAKM antibody (ab8116) at 1 µg/mL

Lane 1:

Mouse spleen tissue lysates

Lane 2:

Rat liver tissue lysate

Predicted band size: 68 kDa

Observed band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human IRAK3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y616

Specificity

Anti-IRAK-M has no cross response to IRAK or IRAK2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "" } } }

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Product details

Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified IgG prepared by ion exchange chromatography.IRAK-M Antibody is affinity chromatography purified via peptide column.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IRAK-M protein also known as IRAKM or interleukin-1 receptor-associated kinase M is a member of the IRAK family and has a molecular mass of approximately 65 kDa. It plays an important role in the immune response regulation. This protein is expressed in monocytes and macrophages but it is also found in other immune cells. Unlike other IRAKs IRAK-M acts as a negative regulator helping to ensure the immune system does not overreact.

Biological function summary

IRAK-M inhibits the signaling pathways that lead to the activation of inflammatory responses. It predominantly affects signaling initiated by toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) preventing excessive inflammatory cytokine production. IRAK-M lacks kinase activity unlike other IRAK family members so it functions as a regulatory molecule rather than participating in kinase-mediated catalysis. It does not form part of a larger complex but interacts directly with other signaling components in these pathways.

Pathways

IRAK-M impacts TLR and IL-1 signaling pathways. These pathways are important in the innate immune system's response to pathogens and inflammation. IRAK-M interacts with proteins such as MyD88 and TNF receptor-associated factor 6 (TRAF6) reducing the downstream production of pro-inflammatory cytokines. This modulation helps to maintain immune system balance and prevent chronic inflammation.

IRAK-M is involved in conditions such as sepsis and autoimmune diseases. In sepsis IRAK-M's alteration can lead to an inadequate immune response influencing the body's ability to control infection. In autoimmune diseases abnormal IRAK-M expression may result in regulatory failures leading to persistent inflammation and tissue damage. IRAK-M's interaction with other proteins like MyD88 suggests its pivotal role in these disorders where misregulated pathways can contribute to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed : 10383454, PubMed : 29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed : 29686383).

See full target information IRAK3

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35743025

2022

Serial Change of Endotoxin Tolerance in a Polymicrobial Sepsis Model.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ji Lee,Jinkun Bae,Jung Ho Lee,Ye Jin Park,Han A Reum Lee,Sehwan Mun,Yun-Seok Kim,Chang June Yune,Tae Nyoung Chung,Kyuseok Kim

Translational andrology and urology 11:268-276 PubMed35280657

2022

Loss of NLRP3 increases bacterial cystitis via IRAKM.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Sun,Lei Xia,Yubing Peng

Bioengineered 12:10147-10159 PubMed34872451

2021

polysaccharides (PSP) improve the palmitic acid (PA)-induced inhibition of survival, inflammation, and glucose uptake in skeletal muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Luo Cai,Xiao-Ping Li,Yi-Lin Zhu,Gang-Qiang Yi,Wei Wang,Xin-Yu Chen,Gui-Ming Deng,Lei Yang,Hu-Zhi Cai,Qiao-Zhen Tong,Li Zhou,Mengying Tian,Xin-Hua Xia,Ping-An Liu

Molecular biology reports 49:121-130 PubMed34757596

2021

MiR-539-5p inhibits the inflammatory injury in septic H9c2 cells by regulating IRAK3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Hu,Hongjun Miao

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 18:1891-1904 PubMed33970466

2021

α-Acetylcholine Receptor Signaling Reduces Neuroinflammation After Subarachnoid Hemorrhage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ari Dienel,Remya A Veettil,Kanako Matsumura,Jude P J Savarraj,H Alex Choi,Peeyush Kumar T,Jaroslaw Aronowski,Pramod Dash,Spiros L Blackburn,Devin W McBride

Cancer management and research 12:7043-7059 PubMed32848462

2020

Hypermethylation of the -Activated MAPK Signaling Pathway to Promote the Development of Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinghai Wu,Yian Ouyang,Bin Wang,Jian Lin,Yun Bai

Oncotarget 8:9053-9066 PubMed28118607

2017

Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A Al-Qahtani,Konstantina Lyroni,Marina Aznaourova,Melpomeni Tseliou,Mashael R Al-Anazi,Mohammed N Al-Ahdal,Saad Alkahtani,George Sourvinos,Christos Tsatsanis

Journal of immunology (Baltimore, Md. : 1950) 197:1776-87 PubMed27481848

2016

Transduction of Functionally Contrasting Signals by Two Mycobacterial PPE Proteins Downstream of TLR2 Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Atul Udgata,Rahila Qureshi,Sangita Mukhopadhyay

PloS one 10:e0129867 PubMed26076454

2015

Induction of Tolerogenic Dendritic Cells by a PEGylated TLR7 Ligand for Treatment of Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoko Hayashi,Shiyin Yao,Brian Crain,Victor J Promessi,Luke Shyu,Caroline Sheng,McNancy Kang,Howard B Cottam,Dennis A Carson,Maripat Corr

Journal of immunology (Baltimore, Md. : 1950) 189:356-64 PubMed22661086

2012

TLR signaling prevents hyperoxia-induced lung injury by protecting the alveolar epithelium from oxidant-mediated death.

Applications

Species

Unspecified reactive species

Megan N Ballinger,Michael W Newstead,Xianying Zeng,Urvashi Bhan,Jeffrey C Horowitz,Bethany B Moore,David J Pinsky,Richard A Flavell,Theodore J Standiford

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