AB32394

Anti-IRAKM antibody [Y278]

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal IRAKM antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Interleukin-1 receptor-associated kinase 3, IRAK-3, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, IRAK-M, IRAK3

3 Images

Lab

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

False colour image of Western blot : Anti-IRAKM antibody [Y278] staining at 1/20000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32394 was shown to bind specifically to IRAKM. A band was observed at 68 kDa in wild-type THP-1 cell lysates with no signal observed at this size in IRAK3 knockout cell line ab281629 (knockout cell lysate ab282979). To generate this image, wild-type and IRAK3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

IRAK3 knockout THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human IRAK3 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-irak3-knockout-thp-1-cell-line-ab281629'>ab281629</a>)

Predicted band size: 68 kDa

Observed band size: 68 kDa

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Lab

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : IRAKM knockout HAP1 cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-IRAKM antibody [Y278] (ab32394)

Predicted band size: 68 kDa

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Unknown

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

All lanes:

Western blot - Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution

All lanes:

A431 cell lysate

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Carrier free

Anti-IRAKM antibody [Y278] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y278

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IRAK-M protein also known as IRAKM or interleukin-1 receptor-associated kinase M is a member of the IRAK family and has a molecular mass of approximately 65 kDa. It plays an important role in the immune response regulation. This protein is expressed in monocytes and macrophages but it is also found in other immune cells. Unlike other IRAKs IRAK-M acts as a negative regulator helping to ensure the immune system does not overreact.

Biological function summary

IRAK-M inhibits the signaling pathways that lead to the activation of inflammatory responses. It predominantly affects signaling initiated by toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs) preventing excessive inflammatory cytokine production. IRAK-M lacks kinase activity unlike other IRAK family members so it functions as a regulatory molecule rather than participating in kinase-mediated catalysis. It does not form part of a larger complex but interacts directly with other signaling components in these pathways.

Pathways

IRAK-M impacts TLR and IL-1 signaling pathways. These pathways are important in the innate immune system's response to pathogens and inflammation. IRAK-M interacts with proteins such as MyD88 and TNF receptor-associated factor 6 (TRAF6) reducing the downstream production of pro-inflammatory cytokines. This modulation helps to maintain immune system balance and prevent chronic inflammation.

IRAK-M is involved in conditions such as sepsis and autoimmune diseases. In sepsis IRAK-M's alteration can lead to an inadequate immune response influencing the body's ability to control infection. In autoimmune diseases abnormal IRAK-M expression may result in regulatory failures leading to persistent inflammation and tissue damage. IRAK-M's interaction with other proteins like MyD88 suggests its pivotal role in these disorders where misregulated pathways can contribute to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors (PubMed : 10383454, PubMed : 29686383). Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex (By similarity). Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells (PubMed : 29686383).

See full target information IRAK3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6752 PubMed36347862

2022

GZMK CD8 T effector memory cells are associated with CD15 neutrophil abundance in non-metastatic colorectal tumors and predict poor clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Tiberti,Carlotta Catozzi,Ottavio Croci,Mattia Ballerini,Danilo Cagnina,Chiara Soriani,Caterina Scirgolea,Zheng Gong,Jiatai He,Angeli D Macandog,Amir Nabinejad,Carina B Nava Lauson,Arianna Quinte',Giovanni Bertalot,Wanda L Petz,Simona P Ravenda,Valerio Licursi,Paola Paci,Marco Rasponi,Luca Rotta,Nicola Fazio,Guangwen Ren,Uberto Fumagalli-Romario,Martin H Schaefer,Stefano Campaner,Enrico Lugli,Luigi Nezi,Teresa Manzo

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 32:305-313 PubMed33025692

2020

IL-1 signaling pathway molecules as key markers in childhood asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenping Wei,Jiaqi Huang,Yu Ma,Xingjie Ma,Ling Fang,Wei Fang,Chuangli Hao

Oncogene 36:1080-1089 PubMed27841867

2016

Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

J M Thompson,Q H Nguyen,M Singh,M W Pavesic,I Nesterenko,L J Nelson,A C Liao,O V Razorenova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRAKM antibody [Y278]

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

Western blot - Anti-IRAKM antibody [Y278] (AB32394)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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