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AB178693

Anti-IRBIT antibody [EPR13409]

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(3 Publications )

Rabbit Recombinant Monoclonal IRBIT antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

DCAL, IRBIT, XPVKONA, AHCYL1, S-adenosylhomocysteine hydrolase-like protein 1, DC-expressed AHCY-like molecule, IP(3)Rs binding protein released with IP(3), Putative adenosylhomocysteinase 2, S-adenosyl-L-homocysteine hydrolase 2, AdoHcyase 2

3 Images
Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (AB178693)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (AB178693)

Intracellular flow cytometrical analysis of permeabilized 293T cells labeling IRBIT with ab178693 at 1/10 (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-IRBIT antibody [EPR13409] (AB178693)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRBIT antibody [EPR13409] (AB178693)

Immunofluorescent analysis of HeLa cells labeling IRBIT with ab178693 at 1/250 (red) and DAPI staining (blue).

Western blot - Anti-IRBIT antibody [EPR13409] (AB178693)
  • WB

Supplier Data

Western blot - Anti-IRBIT antibody [EPR13409] (AB178693)

All lanes:

Western blot - Anti-IRBIT antibody [EPR13409] (ab178693) at 1/10000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Ramos lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

false

  • Carrier free

    Anti-IRBIT antibody [EPR13409] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13409

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRBIT also known as Inositol 145-Trisphosphate Receptor Binding Protein plays significant role in intracellular signaling. It interacts with the inositol 145-trisphosphate receptor (IP3R) to modulate calcium release from the endoplasmic reticulum. The protein has a molecular mass of approximately 54 kDa. IRBIT is expressed in a variety of tissues including the brain pancreas and heart highlighting its broad functional relevance in different physiological systems.
Biological function summary

IRBIT regulates diverse cellular processes influencing calcium signaling pathways and cell cycle progression. It acts as a regulatory subunit and forms a complex with IP3R modulating calcium influx based on cellular demands. Additionally IRBIT associates with other proteins such as the sodium bicarbonate cotransporter NBCe1-B facilitating ion transport and pH regulation which are essential for maintaining cellular homeostasis.

Pathways

IRBIT's role is essential in maintaining calcium balance and signal transduction. It participates in the calcium signaling pathway where its interaction with IP3R proves important for accurate calcium release and uptake. Moreover it is implicated in the bicarbonate transport pathway through its association with NBCe1-B linking to the regulation of ion transport and pH balance in cells processes critical for maintaining cellular function and communication.

An imbalance in IRBIT function associates with certain pathological conditions such as cystic fibrosis and cancer. In cystic fibrosis improper regulation of bicarbonate transport partly due to IRBIT affects mucus function. Additionally in cancer disruptions in calcium signaling pathways involving IRBIT can promote tumorigenesis. Pathway interactions with proteins like IP3R and NBCe1-B illustrate its significant contributions to disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate, competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. Promotes the formation of contact points between the endoplasmic reticulum (ER) and mitochondria, facilitating transfer of Ca(2+) from the ER to mitochondria (PubMed : 27995898). Under normal cellular conditions, functions cooperatively with BCL2L10 to limit ITPR1-mediated Ca(2+) release but, under apoptotic stress conditions, dephosphorylated which promotes dissociation of both AHCYL1 and BCL2L10 from mitochondria-associated endoplasmic reticulum membranes, inhibits BCL2L10 interaction with ITPR1 and leads to increased Ca(2+) transfer to mitochondria which promotes apoptosis (PubMed : 27995898). In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (PubMed : 16793548). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates from ITPR1 to interact with CFTR and SLC26A6, mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (By similarity). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (PubMed : 16769890). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (By similarity). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2 (PubMed : 20584908). Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition (PubMed : 18829453). May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state (PubMed : 19224921). Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (PubMed : 25237103). In vitro does not exhibit any S-adenosyl-L-homocysteine hydrolase activity (By similarity).
See full target information AHCYL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:5054324 PubMed35578598

2022

AHCYL1 Is a Novel Biomarker for Predicting Prognosis and Immunotherapy Response in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xubin Li,Mengqiao Zhang,Xue Yu,Mei Xue,Xiaowei Li,Chao Ma,Wei Jia,Qiang Gao,Chunbo Kang

Molecular & cellular proteomics : MCP 17:175-189 PubMed29133510

2017

Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa García-Berrocoso,Víctor Llombart,Laura Colàs-Campàs,Alexandre Hainard,Virginie Licker,Anna Penalba,Laura Ramiro,Alba Simats,Alejandro Bustamante,Elena Martínez-Saez,Francesc Canals,Jean-Charles Sanchez,Joan Montaner

The Journal of neuroscience : the official journal of the Society for Neuroscience 36:1386-400 PubMed26818524

2016

Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Kruse,Oscar Vivas,Alexis Traynor-Kaplan,Bertil Hille
View all publications
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Product promise

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