AB308387

Anti-IRE1 alpha (phospho Ser724) antibody [HL1885]

Recombinant
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Rabbit Recombinant Monoclonal IRE1 phospho S724 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ERN1 phospho S724.

View Alternative Names

IRE1, ERN1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, hIRE1p, IRE1a

1 Images

Supplier Data

Western blot - Anti-IRE1 alpha (phospho Ser724) antibody [HL1885] (AB308387)

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody [HL1885] (ab308387) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-IRE1 alpha (phospho Ser724) antibody [HL1885] (ab308387) at 1/1000 dilution

Lane 1:

Untreated (–) HepG2 whole cell extracts at 30 µg

Lane 2:

Treated (+) HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1885

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human ERN1 phospho S724. The exact immunogen used to generate this antibody is proprietary information.

O75460

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Reactivity data

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.

Biological function summary

IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.

Pathways

IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.

IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed : 11175748, PubMed : 11779464, PubMed : 12637535, PubMed : 21317875, PubMed : 28128204, PubMed : 30118681, PubMed : 9637683). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed : 21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed : 21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed : 21884936, PubMed : 28067262).

See full target information ERN1 phospho S724

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRE1 alpha (phospho Ser724) antibody [HL1885]

Western blot - Anti-IRE1 alpha (phospho Ser724) antibody [HL1885] (AB308387)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise