AB235171

Anti-IRE1 antibody

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(3 Publications)

Rabbit Polyclonal IRE1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications.

View Alternative Names

Ire1, Ern1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a

2 Images

Supplier Data

Western blot - Anti-IRE1 antibody (AB235171)

7.5% gel.

All lanes:

Western blot - Anti-IRE1 antibody (ab235171) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 35 µg

Lane 2:

C2C12 (Mouse myoblast cell line) cell lysate at 35 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/70000 dilution

Predicted band size: 110 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-IRE1 antibody (AB235171)

All lanes:

Western blot - Anti-IRE1 antibody (ab235171) at 1/1000 dilution

Lane 1:

IRE1 overexpressing HEK293T CMYC/DDK tagged lysate at 10 µg

Lane 2:

HEK293T empty vector lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/70000 dilution

Predicted band size: 110 kDa

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.

Biological function summary

IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.

Pathways

IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.

IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed : 11850408, PubMed : 25164867). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed : 25164867). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed : 11850408, PubMed : 25164867). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed : 11850408, PubMed : 25164867). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (By similarity).

See full target information Ern1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:328 PubMed41044542

2025

Tetrahydropalmatine has analgesic role in mouse model of bone cancer pain by inactivating the TNF-α/uPA/PAR2/TRPV1 pathway in dorsal root ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Guangda Zheng,Linghan Meng,Lu Shang,Juanxia Ren,Dongtao Li,Yanju Bao

Cellular and molecular biology (Noisy-le-Grand, France) 70:226-232 PubMed38372090

2024

Inhibition of RXRA-mediated PLA2G2A improves delirium in COPD mice by regulating endoplasmic reticulum stress pathway and inhibiting cell apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Zhang,Guodong Wang

iScience 26:107043 PubMed37360693

2023

Targeting extracellular CIRP with an X-aptamer shows therapeutic potential in acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wuming Liu,Jianbin Bi,Yifan Ren,Huan Chen,Jia Zhang,Tao Wang,Mengzhou Wang,Lin Zhang,Junzhou Zhao,Zheng Wu,Yi Lv,Bing Liu,Rongqian Wu

View all publications

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Product promise

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Anti-IRE1 antibody

Western blot - Anti-IRE1 antibody (AB235171)

Western blot - Anti-IRE1 antibody (AB235171)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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