Anti-IRE1 antibody [EPR29162-31] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-IRE1 antibody [EPR29162-31] - BSA and Azide free (AB322062)

This data was developed using ab322061, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IRE1 antibody [EPR29162-31] (<a href='/en-us/products/primary-antibodies/ire1-antibody-epr29162-31-ab322061'>ab322061</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting IRE1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-IRE1 antibody [EPR29162-31] - BSA and Azide free (AB322062)

This data was developed using ab322061, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 130 kDa in lanes 1-3 are unknown.

Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 and lanes 3-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

This blot of lanes 1-2 was developed using a high sensitivity ECL substrate.

Exposure time : Lanes1-2 : 92 seconds; Lanes 3-5 : 180 seconds

All lanes:

Western blot - Anti-IRE1 antibody [EPR29162-31] (<a href='/en-us/products/primary-antibodies/ire1-antibody-epr29162-31-ab322061'>ab322061</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse testis tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa

false

• WB

Supplier Data

Western blot - Anti-IRE1 antibody [EPR29162-31] - BSA and Azide free (AB322062)

This data was developed using ab322061, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands lower than 130 kDa are unknown.

All lanes:

Western blot - Anti-IRE1 antibody [EPR29162-31] (<a href='/en-us/products/primary-antibodies/ire1-antibody-epr29162-31-ab322061'>ab322061</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 4:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 6:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa

false

Exposure time: 81s