AB288371

Anti-IRE1 (phospho S724) antibody [1F12]

Name: Anti-IRE1 (phospho S724) antibody [1F12] (ab288371) | Abcam
Brand: Abcam Limited
SKU: AB288371
Rating: 5 (2 reviews)

Recombinant
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Rabbit Recombinant Monoclonal IRE1 phospho S724 antibody. Suitable for ICC, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ERN1 phospho S724.

View Alternative Names

IRE1, ERN1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, hIRE1p, IRE1a

2 Images

Supplier Data

Immunocytochemistry - Anti-IRE1 (phospho S724) antibody [1F12] (AB288371)

Immunofluorescence staining of HeLa cells with ab288371 at 1 : 100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488^®-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supplier Data

Western blot - Anti-IRE1 (phospho S724) antibody [1F12] (AB288371)

All lanes:

Western blot - Anti-IRE1 (phospho S724) antibody [1F12] (ab288371) at 0.75 µg/mL

Lane 1:

HEK293 whole cell lysate (treated with Calyculin A 100nM/60 min)

Lane 2:

HEK293 whole cell lysate

Lane 3:

A549 whole cell lysate (treated with Calyculin A 100nM/30 min)

Lane 4:

A549 whole cell lysate

Lane 5:

HeLa whole cell lysate (treated with EGF 100ng/mL/20 min)

Lane 6:

HeLa whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

1F12

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human ERN1 phospho S724. The exact immunogen used to generate this antibody is proprietary information.

O75460

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/200", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.

Biological function summary

IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.

Pathways

IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.

IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed : 11175748, PubMed : 11779464, PubMed : 12637535, PubMed : 21317875, PubMed : 28128204, PubMed : 30118681, PubMed : 9637683). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed : 21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed : 21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed : 21884936, PubMed : 28067262).

See full target information ERN1 phospho S724

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRE1 (phospho S724) antibody [1F12]

Immunocytochemistry - Anti-IRE1 (phospho S724) antibody [1F12] (AB288371)

Western blot - Anti-IRE1 (phospho S724) antibody [1F12] (AB288371)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise