AB243665

Anti-IRE1 (phospho S724) antibody [EPR23107-16]

Name: Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665) | Abcam
Brand: Abcam Limited
SKU: AB243665
Price: 210 USD
Availability: InStock
Rating: 4 (3 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(3 Reviews)

(13 Publications)

Rabbit Recombinant Monoclonal IRE1 phospho S724 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide - Human samples. Cited in 13 publications.

View Alternative Names

IRE1, ERN1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, hIRE1p, IRE1a

3 Images

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Immunoprecipitation - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (AB243665)

IRE1 (phospho S724) was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10mM DTT for 1h whole cell lysate 10μg with ab243665 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243665 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa treated with 10mM DTT for 1h whole cell lysate 10μg
Lane 2 : ab243665 IP in HeLa treated with 10mM DTT for 1h whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243665 in HeLa treated with 10mM DTT for 1h whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 mins.

Phosphorylation of IRE1 at S724 can be induced by DTT treatment (PMID : 31453810).

All lanes:

Immunoprecipitation - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665)

Predicted band size: 110 kDa,87 kDa

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Lab

Western blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (AB243665)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Phosphorylation of IRE1 at S724 can be induced by DTT treatment (PMID : 31453810).

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HeLa treated with 10mM DTT for 1 hour whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

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Unknown

Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (AB243665)

Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (ab243665 at 1/1,000 dilution).

Lane 1 : IRE1 phospho S724 peptide.

Lane 2 : IRE1 non-phospho peptide.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 mins.

Secondary used is Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100,000 dilution.

Carrier free

Anti-IRE1 (phospho S724) antibody [EPR23107-16] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23107-16

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.

Biological function summary

IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.

Pathways

IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.

IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed : 11175748, PubMed : 11779464, PubMed : 12637535, PubMed : 19328063, PubMed : 21317875, PubMed : 28128204, PubMed : 30118681, PubMed : 36739529, PubMed : 9637683). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed : 21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed : 21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed : 21884936, PubMed : 28067262).

See full target information ERN1 pS724

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:6504-6521 PubMed40950286

2025

NLBK alleviates airway remodeling in COPD by inhibiting RXRA-mediated transcription of PLA2G2A.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Zhang,Fang Fang,Dan Wu,Guodong Wang

Nature communications 16:8155 PubMed40890111

2025

Multiple myeloma associated long non-coding RNA PLUM confers chemoresistance by enhancing PRC2 mediated UPR pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kamalakshi Deka,Jean-Michel Carter,Akash Bahai,Daniel Aron Ang,Nicholas Sim,Hooi Yan Chong,Guan Hwee Bernard Lee,Suet Mien Tan,Wee Joo Chng,Dennis Kappei,Yinghui Li

Lipids in health and disease 24:138 PubMed40221727

2025

High-fat stimulation induces atrial structural remodeling via the TPM1/P53/SHISA5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yansong Cui,Shuting Bai,Zhenlin Liu,Haifeng Ding,Kuan Li,Zhan Li,Yinglong Hou

Open life sciences 20:20220951 PubMed39926475

2025

Preliminary analysis of the role of small hepatitis B surface proteins mutations in the pathogenesis of occult hepatitis B infection via the endoplasmic reticulum stress-induced UPR-ERAD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengrong Huang,Hao Zhang,Jing Wang,Jianfei Li,Qian Liu,Qiyin Zong,Yunyun Zhang,Qin Wang,Qiang Zhou

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400340 PubMed39229920

2024

Zosuquidar Promotes Antitumor Immunity by Inducing Autophagic Degradation of PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Ding,Hongjie Guo,Jie Zhang,Mingming Zheng,Wenjie Zhang,Longsheng Wang,Qianqian Du,Chen Zhou,Yanjun Xu,Honghai Wu,Qiaojun He,Bo Yang

Experimental and therapeutic medicine 28:385 PubMed39161618

2024

Peroxisome proliferator‑activated receptor γ alleviates human umbilical vein endothelial cell injury in deep vein thrombosis by blocking endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxin Zhang,Yongshuai Ge,Liyuan Tao,Mingyuan Liu,Wei Jia,Xuan Tian,Peng Jiang,Zhiyuan Cheng,Jinyong Li,Jianlong Liu

Acta neuropathologica communications 11:154 PubMed37749651

2023

Rapid isolation of intact retinal astrocytes: a novel approach.

Applications

Unspecified application

Species

Unspecified reactive species

Paul F Cullen,Arpan G Mazumder,Daniel Sun,John G Flanagan

Cell death discovery 8:133 PubMed35347123

2022

GRP78 blockade overcomes intrinsic resistance to UBA1 inhibitor TAK-243 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Runqiu Wu,Cong Tian,Wanzhou Wang,Lingni Zhou,Tongxuan Guo,Jiefeng Yu,Changyong Wu,Yang Shen,Xuejiao Liu,Rutong Yu

iScience 25:103973 PubMed35281739

2022

Paracrine signal emanating from stressed cardiomyocytes aggravates inflammatory microenvironment in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Namrita Kaur,Andrea Ruiz-Velasco,Rida Raja,Gareth Howell,Jessica M Miller,Riham R E Abouleisa,Qinghui Ou,Kimberly Mace,Susanne S Hille,Norbert Frey,Pablo Binder,Craig P Smith,Helene Fachim,Handrean Soran,Eileithyia Swanton,Tamer M A Mohamed,Oliver J Müller,Xin Wang,Jonathan Chernoff,Elizabeth J Cartwright,Wei Liu

Cellular and molecular life sciences : CMLS 78:7061-7080 PubMed34636989

2021

Genome-wide mRNA profiling identifies X-box-binding protein 1 (XBP1) as an IRE1 and PUMA repressor.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Gebert,Aleksandra Sobolewska,Sylwia Bartoszewska,Aleksandra Cabaj,David K Crossman,Jarosław Króliczewski,Piotr Madanecki,Michał Dąbrowski,James F Collawn,Rafal Bartoszewski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRE1 (phospho S724) antibody [EPR23107-16]

Immunoprecipitation - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (AB243665)

Western blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (AB243665)

Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR23107-16] (AB243665)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise