AB181153

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854]

RabMAb
Recombinant
What is this?

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(19 Publications)

Rabbit Recombinant Monoclonal IREB2/IRP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 19 publications.

View Alternative Names

Iron-responsive element-binding protein 2, IRE-BP 2, Iron regulatory protein 2, IRP2, IREB2, IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1

5 Images

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling IREB2 (red) with ab181153 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

Lab

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 105 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

All lanes:

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/5000 dilution

All lanes:

Human fetal liver cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 105 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

All lanes:

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 105 kDa

Observed band size: 90 kDa

false

Lab

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Blocking and diluting buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution

Lane 1:

Control HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

HEK293 (Human epithelial cell line from embryonic kidney) transfected with Myc-DDK tagged IREB2 expression vector, whole cell lysate at 20 µg

Lane 3:

Full-length Recombinant Human Aconitase 1 protein, at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 106 kDa

false

Carrier free

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13854

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IREB2 also known as IRP2 and ACO1 also known as Aconitase 1 serve vital functions in iron metabolism. Mechanically IREB2/IRP2 acts as an iron-responsive element-binding protein and is involved in the post-transcriptional regulation of key genes including those encoding transferrin receptor and ferritin. ACO1 functions as a bifunctional protein that switches between being an enzyme and a regulatory protein based on iron levels known to be around 98 kDa in mass. Both of these proteins express in various tissues highlighting their importance across different biological systems.

Biological function summary

The IREB2/IRP2 and ACO1 maintain cellular iron homeostasis by responding to iron levels and regulating the iron-responsive element system. They do not form part of a larger protein complex but operate independently to modulate iron metabolism. When iron is ample ACO1 behaves as an enzyme catalyzing the isomerization of citrate to isocitrate in the citric acid cycle. Conversely under iron-depleted conditions ACO1 joins IREB2/IRP2 in an RNA-binding form to control iron uptake and storage.

Pathways

These proteins play significant roles in the iron regulatory network and the citric acid cycle. IREB2/IRP2 associates with pathways involving iron uptake and release influencing genes related to ferritin and transferrin receptor. ACO1's dual function ties it to both the citric acid cycle and iron regulatory pathways showing its involvement in cellular energy production and iron balance. The interaction with other important proteins such as transferrin and ferritin is important for their regulatory functions within these pathways.

IREB2 and ACO1 connections are apparent in conditions like neurodegenerative diseases and anemia. Abnormal regulation or mutations in these proteins show associations with disorders such as Parkinson’s disease and sideroblastic anemia. Interactions between IREB2/IRP2 and proteins like PARK7/DJ-1 and oxidative stress proteins underline their significance in the pathogenesis of neurodegenerative diseases and issues of iron mismanagement.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

See full target information Iron-responsive element-binding protein 2

Additional targets

ACO1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4195 PubMed38760351

2024

Ferritinophagy mediates adaptive resistance to EGFR tyrosine kinase inhibitors in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Qianfan Hu,Yuzhong Chen,Xing Huang,Yipeng Feng,Yuanjian Shi,Rutao Li,Xuewen Yin,Xuming Song,Yingkuan Liang,Te Zhang,Lin Xu,Gaochao Dong,Feng Jiang

Journal of biochemical and molecular toxicology 38:e23683 PubMed38483099

2024

Excess iron accumulation mediated senescence in diabetic kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xueer Cheng,Yanyan Li,Li Chen,Chunjie Jiang,Shufen Peng,Ping Yao,Yuhan Tang

Acta pharmacologica Sinica 45:268-281 PubMed37674042

2023

Cell senescence induced by toxic interaction between α-synuclein and iron precedes nigral dopaminergic neuron loss in a mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Qing Shen,Xian-Hui Jv,Xi-Zhen Ma,Chong Li,Lin Liu,Wen-Ting Jia,Le Qu,Lei-Lei Chen,Jun-Xia Xie

Methods in molecular biology (Clifton, N.J.) 2712:211-222 PubMed37578709

2023

Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhong,Ruochan Chen

American journal of physiology. Heart and circulatory physiology 324:H212-H225 PubMed36563009

2022

Deferoxamine prevents poststroke memory impairment in female diabetic rats: potential links to hemorrhagic transformation and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiguo Li,Yasir Abdul,Raghavendar Chandran,Sarah Jamil,Rebecca A Ward,Mohammed Abdelsaid,Guangkuo Dong,Susan C Fagan,Adviye Ergul

Scientific reports 12:17484 PubMed36261501

2022

Mesenchymal stem cell bioenergetics and apoptosis are associated with risk for bronchopulmonary dysplasia in extremely low birth weight infants.

Applications

Unspecified application

Species

Unspecified reactive species

Snehashis Hazra,Rui Li,Bianca M Vamesu,Tamas Jilling,Scott W Ballinger,Namasivayam Ambalavanan,Jegen Kandasamy

Frontiers in molecular biosciences 8:660072 PubMed34026834

2021

Cox4i2 Triggers an Increase in Reactive Oxygen Species, Leading to Ferroptosis and Apoptosis in HHV7 Infected Schwann Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Chang,Haochen Guan,Xueyi Wang,Zheng Chen,Wanchun Zhu,Xiangyu Wei,Shiting Li

Blood 137:2090-2102 PubMed33529321

2021

Defective palmitoylation of transferrin receptor triggers iron overload in Friedreich ataxia fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Floriane Petit,Anthony Drecourt,Michaël Dussiot,Coralie Zangarelli,Olivier Hermine,Arnold Munnich,Agnès Rötig

Translational stroke research 12:615-630 PubMed32875455

2020

Deferoxamine Treatment Prevents Post-Stroke Vasoregression and Neurovascular Unit Remodeling Leading to Improved Functional Outcomes in Type 2 Male Diabetic Rats: Role of Endothelial Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yasir Abdul,Weiguo Li,Rebecca Ward,Mohammed Abdelsaid,Sherif Hafez,Guangkuo Dong,Sarah Jamil,Victoria Wolf,Maribeth H Johnson,Susan C Fagan,Adviye Ergul

Redox biology 32:101483 PubMed32169822

2020

Identification of Frataxin as a regulator of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Du,Yi Zhou,Yanchun Li,Jun Xia,Yongjian Chen,Sufeng Chen,Xin Wang,Weidong Sun,Tongtong Wang,Xueying Ren,Xu Wang,Yihan An,Kang Lu,Wanye Hu,Siyuan Huang,Jianghui Li,Xiangmin Tong,Ying Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854]

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (19)

Product promise