Rabbit Recombinant Monoclonal IREB2/IRP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 13 publications.
Images
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img54809.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)")
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img125990.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)")
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img61617.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)")
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img80540.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)")
![Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--flow-cytometry-intracellular-img413933.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (AB181153)")
Publications
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- Methods in molecular biology (Clifton, N.J.) 2712:211-2222023Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Zhong,Ruochan ChenPubMed 37578709
- Frontiers in molecular biosciences 8:6600722021Cox4i2 Triggers an Increase in Reactive Oxygen Species, Leading to Ferroptosis and Apoptosis in HHV7 Infected Schwann Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBowen Chang,Haochen Guan,Xueyi Wang,Zheng Chen,Wanchun Zhu,Xiangyu Wei,Shiting LiPubMed 34026834
- Translational stroke research 12:615-6302020Deferoxamine Treatment Prevents Post-Stroke Vasoregression and Neurovascular Unit Remodeling Leading to Improved Functional Outcomes in Type 2 Male Diabetic Rats: Role of Endothelial Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasir Abdul,Weiguo Li,Rebecca Ward,Mohammed Abdelsaid,Sherif Hafez,Guangkuo Dong,Sarah Jamil,Victoria Wolf,Maribeth H Johnson,Susan C Fagan,Adviye ErgulPubMed 32875455
- Redox biology 32:1014832020Identification of Frataxin as a regulator of ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Du et. al.PubMed 32169822
- Cell reports 30:1417-1433.e72020Systematic Identification of Regulators of Oxidative Stress Reveals Non-canonical Roles for Peroxisomal Import and the Pentose Phosphate Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael M Dubreuil,David W Morgens,Kanji Okumoto,Masanori Honsho,Kévin Contrepois,Brittany Lee-McMullen,Gavin McAllister Traber,Ria S Sood,Scott J Dixon,Michael P Snyder,Yukio Fujiki,Michael C BassikPubMed 32023459
- Toxicology and applied pharmacology 344:742018Corrigendum to "Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBα/β activation in aryl hydrocarbon receptor-elicited hepatotoxicity" [Toxicol. Appl. Pharmacol. 321 (2017) 1-17].Applications:Unspecified applicationReactive species:Unspecified reactive speciesK A Fader et. al.PubMed 29501526
- Journal of Cancer 8:3933-39382017as a Novel Hypoxia-Induced Gene in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIwona Ziółkowska-Suchanek et. al.PubMed 29187867
- Nutrients 9:2017Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats.
- Journal of lipid research 58:1548-15602017Effect of iron on cholesterol 7α-hydroxylase expression in alcohol-induced hepatic steatosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Liang et. al.PubMed 28536109
- The European respiratory journal 49:2017IRP2 as a potential modulator of cell proliferation, apoptosis and prognosis in nonsmall cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeena Khiroya et. al.PubMed 28404645
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Tested | Expected | Not recommended |
| Rat | Tested | Expected | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/2000 | Notes - |
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
Additional Targets
ACO1
Alternative names
Iron-responsive element-binding protein 2, IRE-BP 2, Iron regulatory protein 2, IRP2, IREB2
Recommended products
Rabbit Recombinant Monoclonal IREB2/IRP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13854
- Purification technique
- Affinity purification Protein A
- Specificity
The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IREB2 also known as IRP2 and ACO1 also known as Aconitase 1 serve vital functions in iron metabolism. Mechanically IREB2/IRP2 acts as an iron-responsive element-binding protein and is involved in the post-transcriptional regulation of key genes including those encoding transferrin receptor and ferritin. ACO1 functions as a bifunctional protein that switches between being an enzyme and a regulatory protein based on iron levels known to be around 98 kDa in mass. Both of these proteins express in various tissues highlighting their importance across different biological systems.
- Biological function summary
The IREB2/IRP2 and ACO1 maintain cellular iron homeostasis by responding to iron levels and regulating the iron-responsive element system. They do not form part of a larger protein complex but operate independently to modulate iron metabolism. When iron is ample ACO1 behaves as an enzyme catalyzing the isomerization of citrate to isocitrate in the citric acid cycle. Conversely under iron-depleted conditions ACO1 joins IREB2/IRP2 in an RNA-binding form to control iron uptake and storage.
- Pathways
These proteins play significant roles in the iron regulatory network and the citric acid cycle. IREB2/IRP2 associates with pathways involving iron uptake and release influencing genes related to ferritin and transferrin receptor. ACO1's dual function ties it to both the citric acid cycle and iron regulatory pathways showing its involvement in cellular energy production and iron balance. The interaction with other important proteins such as transferrin and ferritin is important for their regulatory functions within these pathways.
- Associated diseases and disorders
IREB2 and ACO1 connections are apparent in conditions like neurodegenerative diseases and anemia. Abnormal regulation or mutations in these proteins show associations with disorders such as Parkinson’s disease and sideroblastic anemia. Interactions between IREB2/IRP2 and proteins like PARK7/DJ-1 and oxidative stress proteins underline their significance in the pathogenesis of neurodegenerative diseases and issues of iron mismanagement.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img54809.jpg "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)")
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
Blocking and diluting buffer: 5% NFDM /TBST
All lanes: Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution
Lane 1: Control HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: HEK293 (Human epithelial cell line from embryonic kidney) transfected with Myc-DDK tagged IREB2 expression vector, whole cell lysate at 20 µg
Lane 3: Full-length Recombinant Human Aconitase 1 protein, at 0.02 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 105 kDa
Observed band size: 106 kDa
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img125990.jpg "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)")
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
All lanes: Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution
Lane 1: A549 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: Human fetal kidney tissue lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 90 kDa
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img61617.jpg "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)")
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/1000 dilution
Lane 1: Human fetal liver tissue lysate at 20 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse heart tissue lysate at 20 µg
Lane 5: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 105 kDa
Observed band size: 90 kDa
![Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--western-blot-img80540.jpg "Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)")
Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
All lanes: Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153) at 1/5000 dilution
All lanes: Human fetal liver cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Predicted band size: 105 kDa
Observed band size: 90 kDa
![Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153), expandable thumbnail](https://content.abcam.com/products/images/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153--flow-cytometry-intracellular-img413933.jpg "Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)")
Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (ab181153)
Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling IREB2 (red) with ab181153 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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