AB250381

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IREB2/IRP2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

View Alternative Names

Iron-responsive element-binding protein 2, IRE-BP 2, Iron regulatory protein 2, IRP2, IREB2, IREB1, ACO1, Cytoplasmic aconitate hydratase, Aconitase, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, Iron-responsive element-binding protein 1, IRP1, IRE-BP 1

5 Images

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

This data was developed using ab181153, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling IREB2 (red) with ab181153 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

Lab

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

This data was developed using ab181153, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] (<a href='/en-us/products/primary-antibodies/ireb2-irp2-aconitase-1-aco1-antibody-epr13854-ab181153'>ab181153</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 105 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

This data was developed using ab181153, the same antibody clone in a different buffer formulation.

All lanes:

Human fetal liver cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 105 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

This data was developed using ab181153, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 105 kDa

Observed band size: 90 kDa

false

Lab

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

All lanes:

Lane 1:

Control HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

HEK293 (Human epithelial cell line from embryonic kidney) transfected with Myc-DDK tagged IREB2 expression vector, whole cell lysate at 20 µg

Lane 3:

Full-length Recombinant Human Aconitase 1 protein, at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 106 kDa

false

Unconjugated

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13854

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody immunogen share 80% homology with the IREB1 protein, therefore it is likely that the antibody cross-react with IREB1. We received some customers feedback where they observed this cross-reactivity, however no experiments were performed in our lab in order to confirm this.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab250381 is the carrier-free version of ab181153.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IREB2 also known as IRP2 and ACO1 also known as Aconitase 1 serve vital functions in iron metabolism. Mechanically IREB2/IRP2 acts as an iron-responsive element-binding protein and is involved in the post-transcriptional regulation of key genes including those encoding transferrin receptor and ferritin. ACO1 functions as a bifunctional protein that switches between being an enzyme and a regulatory protein based on iron levels known to be around 98 kDa in mass. Both of these proteins express in various tissues highlighting their importance across different biological systems.

Biological function summary

The IREB2/IRP2 and ACO1 maintain cellular iron homeostasis by responding to iron levels and regulating the iron-responsive element system. They do not form part of a larger protein complex but operate independently to modulate iron metabolism. When iron is ample ACO1 behaves as an enzyme catalyzing the isomerization of citrate to isocitrate in the citric acid cycle. Conversely under iron-depleted conditions ACO1 joins IREB2/IRP2 in an RNA-binding form to control iron uptake and storage.

Pathways

These proteins play significant roles in the iron regulatory network and the citric acid cycle. IREB2/IRP2 associates with pathways involving iron uptake and release influencing genes related to ferritin and transferrin receptor. ACO1's dual function ties it to both the citric acid cycle and iron regulatory pathways showing its involvement in cellular energy production and iron balance. The interaction with other important proteins such as transferrin and ferritin is important for their regulatory functions within these pathways.

IREB2 and ACO1 connections are apparent in conditions like neurodegenerative diseases and anemia. Abnormal regulation or mutations in these proteins show associations with disorders such as Parkinson’s disease and sideroblastic anemia. Interactions between IREB2/IRP2 and proteins like PARK7/DJ-1 and oxidative stress proteins underline their significance in the pathogenesis of neurodegenerative diseases and issues of iron mismanagement.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

See full target information Iron-responsive element-binding protein 2

Additional targets

ACO1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

Western blot - Anti-IREB2/IRP2 + Aconitase 1/ACO1 antibody [EPR13854] - BSA and Azide free (AB250381)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise