AB232994

Anti-IREB2/IRP2 antibody

Name: Anti-IREB2/IRP2 antibody (ab232994) | Abcam
Brand: Abcam Limited
SKU: AB232994

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Polyclonal IREB2/IRP2 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Iron-responsive element-binding protein 2 aa 1-300.

View Alternative Names

Iron-responsive element-binding protein 2, IRE-BP 2, Iron regulatory protein 2, IRP2, IREB2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for IREB2/IRP2 using ab232994 at 10μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

Formalin-fixed, paraffin-embedded human skin cancer tissue stained for IREB2/IRP2 using ab232994 at 10μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

Formalin-fixed, paraffin-embedded human kidney tissue stained for IREB2/IRP2 using ab232994 at 10μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-IREB2/IRP2 antibody (AB232994)

All lanes:

Western blot - Anti-IREB2/IRP2 antibody (ab232994) at 3 µg/mL

Lane 1:

Pig liver tissue lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

Human lung tissue lysate

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 105 kDa

true

Supplier Data

Western blot - Anti-IREB2/IRP2 antibody (AB232994)

All lanes:

Western blot - Anti-IREB2/IRP2 antibody (ab232994) at 3 µg/mL

All lanes:

Recombinant human IREB2/IRP2

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 105 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Iron-responsive element-binding protein 2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P48200

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Iron Regulatory Protein 2 (IREB2) also known as Iron Responsive Element Binding Protein 2 (IRP2) is an essential regulator of cellular iron homeostasis. IREB2 mechanically binds to iron-responsive elements (IREs) on mRNA controlling the post-transcriptional expression of target genes involved in iron metabolism. The protein has a molecular weight of approximately 105 kDa and is expressed in various tissues including the liver brain and pancreas. Its regulation is strongly controlled by iron availability with high iron levels leading to its degradation.

Biological function summary

IREB2 regulates the stability and translation of mRNAs encoding proteins linked to iron uptake storage and utilization. It plays an important role outside of a complex acting primarily on its own to bind transcripts that include ferritin and transferrin receptor mRNAs. Its activity increases under conditions of low iron availability promoting iron uptake and reducing iron sequestration to meet metabolic needs. Through these mechanisms IREB2 helps maintain iron supply for essential enzymes and cellular processes.

Pathways

IREB2 acts as a central player in the iron metabolism pathway influencing both iron import and storage. The pathways it participates in are essential for maintaining cellular and systemic iron balance. It works in coordination with IRP1 forming a regulatory network that adjusts iron homeostasis. Under varying iron concentrations IREB2 modulates expression of its target genes involved in iron transport and storage within the framework of the iron-sulfur cluster biosynthesis pathway.

Aberrant IREB2 activity links to neurodegenerative diseases such as Alzheimer's disease where iron dysregulation is often observed. Additionally IREB2 is associated with hemochromatosis a condition characterized by iron overload. In the context of these diseases IREB2 interacts with proteins such as APP in Alzheimer's and HFE in hemochromatosis influencing their role in disease progression and shedding light on potential therapeutic targets for maintaining iron balance.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

See full target information Iron-responsive element-binding protein 2

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 29:498 PubMed39415292

2024

The deubiquitinase OTUD3 stabilizes IRP2 expression to reduce hippocampal neuron ferroptosis via the p53/PTGS2 pathway to ameliorate cerebral ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Hou,Yujie Hu,Tian Yun,Hongxin Li,Guoshuai Yang,Dan Yu

Discover oncology 15:401 PubMed39225900

2024

Hsa_circYARS interacts with miR-29a-3p to up-regulate IREB2 and promote laryngeal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhao Guo,Yuxia Zhao,Naicai Guo,Meng Xu,Xiaolei Wang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23876 PubMed39120539

2024

Hypoxia-inducible factor-1α can reverse the Adriamycin resistance of breast cancer adjuvant chemotherapy by upregulating transferrin receptor and activating ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Yu,Qingqun Guo,Haojie Zhang,Xiaohong Wang,Yong Han,Zhenlin Yang

Bioactive materials 25:615-628 PubMed37056264

2023

Hydrogel platform with tunable stiffness based on magnetic nanoparticles cross-linked GelMA for cartilage regeneration and its intrinsic biomechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Zhou,Chunli Wang,Kang Xu,Zhixing Niu,Shujuan Zou,Demao Zhang,Zhiyong Qian,Jinfeng Liao,Jing Xie

Oncology letters 23:188 PubMed35527785

2022

Intratumoral expression analysis reveals that OX40 and TIM-3 are prominently expressed and have variable associations with clinical outcomes in high grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole E James,Ashley D Valenzuela,Jenna B Emerson,Morgan Woodman,Katherine Miller,Virginia Hovanesian,Joyce Ou,Jennifer R Ribeiro

Cell death & disease 12:839 PubMed34497268

2021

CISD3 inhibition drives cystine-deprivation induced ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Li,Xin Wang,Zhihui Huang,Yi Zhou,Jun Xia,Wanye Hu,Xu Wang,Jing Du,Xiangmin Tong,Ying Wang

Oncology letters 22:657 PubMed34386079

2021

Iron regulatory protein 1 promotes ferroptosis by sustaining cellular iron homeostasis in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fengping Yao,Xiaohong Cui,Ying Zhang,Zhuchun Bei,Hongquan Wang,Dongxu Zhao,Hong Wang,Yongfei Yang

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IREB2/IRP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IREB2/IRP2 antibody (AB232994)

Western blot - Anti-IREB2/IRP2 antibody (AB232994)

Western blot - Anti-IREB2/IRP2 antibody (AB232994)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise