Rabbit Polyclonal IRF1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Irf1 aa 1 to C-terminus.
Images
Publications
Filter by
- International journal of biological sciences 18:995-10072022Ascorbic acid induced TET2 enzyme activation enhances cancer immunotherapy efficacy in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDing Peng,Anbang He,Shiming He,Guangzhe Ge,Shuo Wang,Weimin Ci,Xuesong Li,Dan Xia,Liqun ZhouPubMed 35173532
- International journal of molecular medicine 47:2021IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Xu,Yong Wu,Ke Yi,Yan Hu,Weiqun Ding,Chungen XingPubMed 33907823
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Irf1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P15314
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses (PubMed:15226432, PubMed:15509808, PubMed:17516545, PubMed:17942705, PubMed:18497060, PubMed:19404407, PubMed:19851330, PubMed:22367195, PubMed:32385160). Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage (PubMed:15226432, PubMed:15509808, PubMed:17516545, PubMed:17942705, PubMed:18497060, PubMed:19404407, PubMed:19851330, PubMed:22367195). Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:15226432, PubMed:15509808, PubMed:17516545, PubMed:17942705, PubMed:18497060, PubMed:19404407, PubMed:19851330, PubMed:21389130, PubMed:22367195). Has an essentail role in IFNG-dependent immunity to mycobacteria (PubMed:36736301). Competes with the transcriptional repressor ZBED2 for binding to a common consensus sequence in gene promoters (PubMed:32385160). Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha/beta, RIGI, TNFSF10/TRAIL, ZBP1, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as GBP2, GBP5 and NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA; metabolic enzymes, such as ACOD1/IRG1 (PubMed:15226432, PubMed:15509808, PubMed:17516545, PubMed:17942705, PubMed:18497060, PubMed:19404407, PubMed:19851330, PubMed:22367195). Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4 (PubMed:18641303, PubMed:22200613). Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53 (PubMed:15509808, PubMed:18084608). Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750). Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development (PubMed:11244049, PubMed:11846971, PubMed:11846974, PubMed:16932750). Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells (PubMed:20049431).
Alternative names
Interferon regulatory factor 1, IRF-1, IRF1
Recommended products
Rabbit Polyclonal IRF1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Irf1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Irf1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P15314
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab232861 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IRF1 also known as Interferon Regulatory Factor 1 is a transcription factor playing an important role in the regulation of interferon genes. This protein has a molecular weight of approximately 37 kDa. IRF1 is most commonly expressed in the nucleus but can be found elsewhere in the cell depending on the context. It operates by binding specific DNA sequences and influencing the transcription of genes involved in immune response.
- Biological function summary
IRF1 influences cell growth arrest apoptosis and immune responses. It acts as an important regulator in the transcription of interferon-alpha and beta genes. IRF1 functions independently and is not generally part of a larger complex although it interacts with other transcription factors to modulate gene expression.
- Pathways
IRF1 plays a significant role in both the type I interferon signaling pathway and the NF-kB pathway. These pathways are essential in the body's response to viral infections and inflammation. IRF1 interacts with proteins like STAT1 and NF-kB to initiate and regulate these immune responses effectively coordinating cellular reactions to external stress signals.
- Associated diseases and disorders
IRF1 has associations with cancer and autoimmune diseases. In several cancer types such as gastric cancer IRF1 expression levels can affect tumor suppression and progression. IRF1 also interacts with proteins like P53 in the context of cancer highlighting its role in immune and apoptotic pathways. In autoimmune conditions like multiple sclerosis altered IRF1 activity can impact disease severity and progression often through its interactions with other immune mediators.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody (ab232861)
Formalin-fixed, paraffin-embedded mouse lung tissue stained for IRF1 with ab232861 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-IRF1 antibody (ab232861)
All lanes: Western blot - Anti-IRF1 antibody (ab232861) at 2 µg/mL
All lanes: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 36 kDa
Western blot - Anti-IRF1 antibody (ab232861)
All lanes: Western blot - Anti-IRF1 antibody (ab232861) at 2 µg/mL
All lanes: Mouse cerebrum lysate
Predicted band size: 36 kDa
Western blot - Anti-IRF1 antibody (ab232861)
All lanes: Western blot - Anti-IRF1 antibody (ab232861) at 2 µg/mL
All lanes: Mouse placenta lysate
Predicted band size: 36 kDa
Western blot - Anti-IRF1 antibody (ab232861)
All lanes: Western blot - Anti-IRF1 antibody (ab232861) at 2 µg/mL
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody (ab232861)
Paraffin-embedded mouse cerebrum tissue stained for IRF1 with ab232861 at 10 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-IRF1 antibody (ab232861)
All lanes: Western blot - Anti-IRF1 antibody (ab232861) at 2 µg/mL
All lanes: Recombinant mouse IRF1 protein
Predicted band size: 36 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com