AB272088

Anti-IRF1 antibody [BLR039F] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal IRF1 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IRF1 aa 250-300.

View Alternative Names

Interferon regulatory factor 1, IRF-1, IRF1

4 Images

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Immunocytochemistry - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Formalin-fixed, paraffin-embedded OVCAR-8 cells labeling IRF1 using ab243895 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for IRF1 using ab243895 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

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Immunoprecipitation - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

IRF1 was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243895 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using ab243895 at 1/1000 dilution.

Lane 1 : ab243895 IP in Jurkat whole cell lysate.

Lane 2 : Control IgG in Jurkat whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

All lanes:

Immunoprecipitation - Anti-IRF1 antibody [BLR039F] (<a href='/en-us/products/primary-antibodies/irf1-antibody-blr039f-ab243895'>ab243895</a>)

Predicted band size: 36 kDa

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Western blot - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Western blot analysis using ab243895 at 1/1000 dilution.

Lane 1 : Jurkat whole cell lysate (50 μg).

Lane 2 : HepG2 whole cell lysate (50 μg).

Lane 3 : HeLA (+ IFN-gamma) whole cell lysate (50 μg).

Lane 4 : HeLa whole cell lysate (50 μg).

Lane 5 : SR whole cell lysate (50 μg).

Lane 6 : MOLT-4 whole cell lysate (50 μg).

Lane 7 : RPMI 8226 whole cell lysate (50 μg).

Lane 8 : KG-1 whole cell lysate (50 μg).

Lane 9 : A549 whole cell lysate (50 μg).

Lane 10 : MCF7 whole cell lysate (50 μg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243895).

All lanes:

Western blot - Anti-IRF1 antibody [BLR039F] (<a href='/en-us/products/primary-antibodies/irf1-antibody-blr039f-ab243895'>ab243895</a>)

Predicted band size: 36 kDa

false

Unconjugated

Anti-IRF1 antibody [BLR039F]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR039F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC, IP

applications

Immunogen

Synthetic Peptide within Human IRF1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P10914

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab272088 is the BSA-free version of ab243895.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF1 also known as Interferon Regulatory Factor 1 is a transcription factor playing an important role in the regulation of interferon genes. This protein has a molecular weight of approximately 37 kDa. IRF1 is most commonly expressed in the nucleus but can be found elsewhere in the cell depending on the context. It operates by binding specific DNA sequences and influencing the transcription of genes involved in immune response.

Biological function summary

IRF1 influences cell growth arrest apoptosis and immune responses. It acts as an important regulator in the transcription of interferon-alpha and beta genes. IRF1 functions independently and is not generally part of a larger complex although it interacts with other transcription factors to modulate gene expression.

Pathways

IRF1 plays a significant role in both the type I interferon signaling pathway and the NF-kB pathway. These pathways are essential in the body's response to viral infections and inflammation. IRF1 interacts with proteins like STAT1 and NF-kB to initiate and regulate these immune responses effectively coordinating cellular reactions to external stress signals.

IRF1 has associations with cancer and autoimmune diseases. In several cancer types such as gastric cancer IRF1 expression levels can affect tumor suppression and progression. IRF1 also interacts with proteins like P53 in the context of cancer highlighting its role in immune and apoptotic pathways. In autoimmune conditions like multiple sclerosis altered IRF1 activity can impact disease severity and progression often through its interactions with other immune mediators.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses (PubMed : 15226432, PubMed : 15509808, PubMed : 17516545, PubMed : 17942705, PubMed : 18497060, PubMed : 19404407, PubMed : 19851330, PubMed : 22367195, PubMed : 32385160). Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage (PubMed : 15226432, PubMed : 15509808, PubMed : 17516545, PubMed : 17942705, PubMed : 18497060, PubMed : 19404407, PubMed : 19851330, PubMed : 22367195, PubMed : 7687740). Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed : 15226432, PubMed : 15509808, PubMed : 17516545, PubMed : 17942705, PubMed : 18497060, PubMed : 19404407, PubMed : 19851330, PubMed : 21389130, PubMed : 22367195). Has an essentail role in IFNG-dependent immunity to mycobacteria (PubMed : 36736301). Competes with the transcriptional repressor ZBED2 for binding to a common consensus sequence in gene promoters (PubMed : 32385160). Its target genes for transcriptional activation activity include : genes involved in anti-viral response, such as IFN-alpha/beta, RIGI, TNFSF10/TRAIL, ZBP1, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as GBP2, GBP5 and NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA; metabolic enzymes, such as ACOD1/IRG1 (PubMed : 15226432, PubMed : 15509808, PubMed : 17516545, PubMed : 17942705, PubMed : 18497060, PubMed : 19404407, PubMed : 19851330, PubMed : 22367195). Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4 (PubMed : 18641303, PubMed : 22200613). Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53 (PubMed : 15509808, PubMed : 18084608). Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells (PubMed : 11244049, PubMed : 11846971, PubMed : 11846974, PubMed : 16932750). Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development (PubMed : 11244049, PubMed : 11846971, PubMed : 11846974, PubMed : 16932750). Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells (PubMed : 20049431).

See full target information IRF1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRF1 antibody [BLR039F] - BSA free

Immunocytochemistry - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Immunoprecipitation - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Western blot - Anti-IRF1 antibody [BLR039F] - BSA free (AB272088)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise