Rabbit Recombinant Monoclonal IRF2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples.
Images
![Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--western-blot-img141618.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109423.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443)")
![Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunocytochemistry-immunofluorescence-img15543.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127205.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60250.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (AB229443)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Alternative names
Interferon regulatory factor 2, IRF-2, IRF2
Recommended products
Rabbit Recombinant Monoclonal IRF2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples.
Alternative names
Interferon regulatory factor 2, IRF-2, IRF2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR4644(2)
- Purification technique
Affinity purification Protein A
- Dissociation constant
4.04 x 10-10 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab229443 is the carrier-free version of Anti-IRF2 antibody [EPR4644(2)] ab124744.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Interferon Regulatory Factor 2 (IRF2) also known as IRF-2 plays an important role in cellular processes like modulation of immune responses and regulation of gene expression. It has a molecular mass of approximately 39 kDa. IRF2 is expressed across various tissues including the immune system cells fibroblasts and epithelial cells. It can function as both a transcriptional activator and repressor depending on the context and specific gene targets.
- Biological function summary
IRF2 regulates immune responses by controlling the expression of interferon (IFN) responsive genes. It acts independently and as part of transcriptional complexes binding to specific DNA sequences within target genes. Its action prevents excessive activation of immune responses maintaining a balanced immune system. IRF2 also influences cell cycle regulation contributing to its role in cell growth and differentiation.
- Pathways
And several cellular communication networks IRF2 is a central figure in the interferon signaling pathway interacting with other members of the IRF family like IRF1 and IRF3. These interactions help propagate signals necessary for effective immune responses. In conjunction with the JAK-STAT pathway IRF2 modulates expressions that control antiviral responses and cellular proliferation showing its critical placement in immune response regulation.
- Associated diseases and disorders
IRF2's dysregulation has associations with cancer and autoimmune diseases. Overexpression of IRF2 is linked to certain cancers providing cells with growth advantages and resistance to apoptosis commonly in concert with proteins like STAT1. In autoimmune diseases IRF2 impacts inflammatory responses with aberrant regulation contributing to conditions like systemic lupus erythematosus. Its involvement highlights the complex nature of immune regulation and potential for targeted therapy interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--western-blot-img141618.jpg "Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
Western blot - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
This WB data was generated using the same anti-IRF2 antibody clone, EPR4644(2), in a different buffer formulation (cat# ab12474).
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: IRF2 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: CACO2 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-IRF2 antibody [EPR4644(2)] ab124744 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.
Anti-IRF2 antibody [EPR4644(2)] ab124744 was shown to recognize IRF2 when IRF2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IRF2 knockout samples were subjected to SDS-PAGE. Anti-IRF2 antibody [EPR4644(2)] ab124744 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-IRF2 antibody [EPR4644(2)] (Anti-IRF2 antibody [EPR4644(2)] ab124744)
Predicted band size: 39 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109423.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
This IHC data was generated using the same anti-IRF2 antibody clone, EPR4644(2), in a different buffer formulation (cat# Anti-IRF2 antibody [EPR4644(2)] ab124744).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labeling IRF2 with purified Anti-IRF2 antibody [EPR4644(2)] ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution was used as the secondary antibody. Nucleus staining on epithelium of human colon was observed. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunocytochemistry-immunofluorescence-img15543.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
Immunocytochemistry/ Immunofluorescence - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IRF2 with Anti-IRF2 antibody [EPR4644(2)] ab124744 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-IRF2 antibody [EPR4644(2)] ab124744 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF2 antibody [EPR4644(2)] ab124744).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127205.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical cancer tissue labeling IRF2 with purified Anti-IRF2 antibody [EPR4644(2)] ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution was used as the secondary antibody. Nucleus staining on tumor cells of human cervix cancer was observed. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF2 antibody [EPR4644(2)] ab124744).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img60250.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labeling IRF2 with purified Anti-IRF2 antibody [EPR4644(2)] ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution was used as the secondary antibody. Nucleus staining on epithelial cells of mouse colon was observed. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF2 antibody [EPR4644(2)] ab124744).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9657.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic carcinoma tissue labelling IRF2 with purified Anti-IRF2 antibody [EPR4644(2)] ab124744 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IRF2 antibody [EPR4644(2)] ab124744).
![OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--oi-rd-scanning-img84202.jpg "OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9657.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
![OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443), expandable thumbnail](https://content.abcam.com/products/images/irf2-antibody-epr46442-bsa-and-azide-free-ab229443--oi-rd-scanning-img84202.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-IRF2 antibody [EPR4644(2)] - BSA and Azide free (ab229443)")
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