AB221099

Anti-IRF2BPL antibody

Name: Anti-IRF2BPL antibody (ab221099) | Abcam
Brand: Abcam Limited
SKU: AB221099
Price: 645 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal IRF2BPL antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IRF2BPL aa 500-600.

View Alternative Names

C14orf4, EAP1, KIAA1865, My039, IRF2BPL, Probable E3 ubiquitin-protein ligase IRF2BPL, Enhanced at puberty protein 1, Interferon regulatory factor 2-binding protein-like

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-IRF2BPL antibody (AB221099)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IRF2BPL antibody (AB221099)

Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100 permeabilized MCF-7 cells labeling IRF2BPL with ab221099 at 4 μg/mL dilution (green).

Supplier Data

Western blot - Anti-IRF2BPL antibody (AB221099)

Western blot analysis labeling IRF2BPL with ab221099 at 0.04 ug/ml.

All lanes:

Western blot - Anti-IRF2BPL antibody (ab221099) at 0.04 µg/mL

Lane 1:

PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lane 2:

Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate

Predicted band size: 83 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IRF2BPL aa 500-600. The exact immunogen used to generate this antibody is proprietary information.

Q9H1B7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRF2BPL also known as Interferon Regulatory Factor 2 Binding Protein Like is a protein with a mass of roughly 86 kDa. This protein is expressed widely in various tissues with notable expression in the central nervous system. Mechanically IRF2BPL functions as a transcriptional regulator. It interacts with DNA influencing the transcription of genes that play roles in cellular processes including cell growth and immune responses.

Biological function summary

IRF2BPL's regulatory activities influence neurodevelopment and neuroprotection. It does not typically form part of larger protein complexes. Instead it modulates gene expression linked to neuronal survival and maturation. The protein involvement in the transcription of genes that affect neuronal function suggests its critical roles in maintaining nervous system health and function.

Pathways

IRF2BPL is intricately involved in the neuronal signaling pathway. It acts alongside proteins like IRF2 where they collectively impact the transcriptional regulation of genes that participate in neuronal differentiation and development. Links with the mTOR signaling pathway also exist with implications for axonal growth and neuroprotection indicating that IRF2BPL bridges cellular growth pathways with specific neuronal processes.

Malfunctions in IRF2BPL are linked to neurodevelopmental disorders such as NEDAMSS (Neurodevelopmental Disorder with Regression Abnormal Movements and Seizures). Mutations in the IRF2BPL gene can lead to severe clinical symptoms including intellectual disability and epilepsy. Connections with proteins like MECP2 further support its role in neurological conditions highlighting the essential nature of IRF2BPL in maintaining normal neural functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable E3 ubiquitin protein ligase involved in the proteasome-mediated ubiquitin-dependent degradation of target proteins (PubMed : 29374064). Through the degradation of CTNNB1, functions downstream of FOXF2 to negatively regulate the Wnt signaling pathway (PubMed : 29374064). Probably plays a role in the development of the central nervous system and in neuronal maintenance (Probable). Also acts as a transcriptional regulator of genes controlling female reproductive function. May play a role in gene transcription by transactivating GNRH1 promoter and repressing PENK promoter (By similarity).

See full target information IRF2BPL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0328698 PubMed40720499

2025

Response Gene to Complement 32 promotes cell proliferation and tamoxifen resistance in breast cancer via elevated FoxM1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlei Li,Yan Liu,Zhiqian Wang,Xiaocui Bu,Yu Wang,Wei Zhang,Peng Zhao

Science advances 8:eabl5613 PubMed35044823

2022

Loss of IRF2BPL impairs neuronal maintenance through excess Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Paul C Marcogliese,Debdeep Dutta,Shrestha Sinha Ray,Nghi D P Dang,Zhongyuan Zuo,Yuchun Wang,Di Lu,Fatima Fazal,Thomas A Ravenscroft,Hyunglok Chung,Oguz Kanca,JiJun Wan,Emilie D Douine,Undiagnosed Diseases Network,Loren D M Pena,Shinya Yamamoto,Stanley F Nelson,Matthew Might,Kathrin C Meyer,Nan Cher Yeo,Hugo J Bellen

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IRF2BPL antibody

Immunocytochemistry/ Immunofluorescence - Anti-IRF2BPL antibody (AB221099)

Western blot - Anti-IRF2BPL antibody (AB221099)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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